Bioseparation of phycocyanin from Phormidium tergestinum using an aqueous two-phase system.

abstract：:Cyanophycin is a bacterial storage polymer for carbon, nitrogen and energy with emerging industrial applications. As efficient cyanophycin production is enhanced by peptone, but commercial peptones are very expensive, thereby increasing the overall production cost, an enzymatically produced feather hydrolysate (FH) is...

journal_title：Preparative biochemistry & biotechnology

authors： Altun M,Wiefel L,Steinbüchel A

更新日期：2018-01-01 00:00:00

abstract：:Conventional completely mixed anaerobic treatment systems limit the chances of the different species of bacteria to spatially group together according to their mutual cooperation and as a result, show a lower efficiency and vulnerability towards shock situations. It is interesting to know about the stratification of t...

journal_title：Preparative biochemistry & biotechnology

authors： Pandey S,Sarkar S

更新日期：2019-01-01 00:00:00

abstract：:The physicochemical properties of tarhana soup produced with different dough treatments, fermentation times, and preservation methods were examined. Tarhana doughs were prepared with yogurt (control) or baker's yeast (Saccharomyces cerevisiae) and fermented for 3 days. Samples were taken at 24, 48, and 72 hr. Samples ...

journal_title：Preparative biochemistry & biotechnology

authors： Gurbuz O,Gocmen D,Ozmen N,Dagdelen F

更新日期：2010-01-01 00:00:00

abstract：:It appears that the reason for the lack of information and data about corn coleoptile lectin is due to the instability of preparation with a rapid loss of hemagglutinating activity and abundant precipitate. In this paper, we assayed phosphate, borate, tris, and ascorbate-sucrose extraction buffers to compare lectin ac...

journal_title：Preparative biochemistry & biotechnology

authors： Martínez M,Córdoba F

更新日期：2000-08-01 00:00:00

abstract：:Our previous studies showed that sulfanilamide is a new competitive inhibitor of and can be used in the purification of lactoperoxidase (LPO, EC1.11.1.7) from milk. However, this method has some disadvantages like a lower purification factor. The aim of the present study is to improve the purification process of milk ...

journal_title：Preparative biochemistry & biotechnology

authors： Köksal Z,Usanmaz H,Bayrak S,Ozdemir H

更新日期：2017-02-07 00:00:00

abstract：:Bioconversion of biodiesel-derived crude glycerol into carotenoids and lipids was investigated by a microbial conversion of an oleaginous red yeast Sporidiobolus pararoseus KM281507. The methanol content in crude glycerol (0.5%, w/v) did not show a significant effect on biomass production by strain KM281507. However, ...

journal_title：Preparative biochemistry & biotechnology

authors： Chaiyaso T,Manowattana A

更新日期：2018-01-02 00:00:00

abstract：:The emergence of drug resistance in Streptococcus pneumoniae (Spn) is a global health threat and necessitates discovery of novel therapeutics. The serine acetyltransferase (also known as CysE) is an enzyme of cysteine biosynthesis pathway and is reported to be essential for the survival of several pathogenic bacteria....

journal_title：Preparative biochemistry & biotechnology

authors： Verma D,Antil M,Gupta V

更新日期：2019-01-01 00:00:00

abstract：:Immunoassay has been widely used in the screening of mycotoxins, which may be hazardous to the operator or the environment. This study was to develop a green way to measure zearalenone (ZEN) with a monoclonal β-type anti-idiotype antibody (Ab2β) against ZEN in place of ZEN standard. Six monoclonal β-type anti-idiotype...

journal_title：Preparative biochemistry & biotechnology

authors： Shi L,Yu T,Luo M,Wang H

更新日期：2020-01-01 00:00:00

abstract：:The effect of glycerol, glucose, and starch as carbon sources on the antioxidant defense system such as superoxide dismutase (SOD) and catalase (CAT) activities, pyruvate levels, and membrane lipid peroxidation (LPO) levels of Streptomyces sp. M4018, after isolation from the rhizosphere samples of Colutea arborescens ...

journal_title：Preparative biochemistry & biotechnology

authors： Kayalı HA,Sazak A,Sahin N,Tarhan L

更新日期：2012-01-01 00:00:00

abstract：:The sequence cato encoding catechol 1,2-dioxygenase from Candida tropicalis JH8 was cloned, sequenced, and expressed in Escherichia coli. The sequence cato contained an ORF of 858 bp encoding a polypeptide of 285 amino acid residues. The recombinant catechol 1,2-dioxygenase exists as a homodimer structure with a subun...

journal_title：Preparative biochemistry & biotechnology

authors： Long Y,Yang S,Xie Z,Cheng L

更新日期：2016-10-02 00:00:00

abstract：:Strain DRP2-19 was detected to produce high yield of glucansucrase in MRS broth, which was identified to be Leuconostoc mesenteroides. In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discov...

journal_title：Preparative biochemistry & biotechnology

authors： Du R,Zhao F,Pan L,Han Y,Xiao H,Zhou Z

更新日期：2018-01-01 00:00:00

abstract：:Psidium guajava leaves are rich in health-promoting flavonoids compounds. For better utilization of the resource, the ultrasound-assisted aqueous extraction was investigated using Box-Behnken design under response surface methodology. A high coefficient of determination (R2 = 97.8%) indicated good agreement between th...

journal_title：Preparative biochemistry & biotechnology

authors： Li J,Wu C,Li F,Yu R,Wu X,Shen L,Liu Y,Zeng W

更新日期：2019-01-01 00:00:00

abstract：:The present investigation describes the comparative properties, particularly the substrate specificity of three kallikrein-like serine proteinases (I, II and III) purified from rat submandibular gland extract (Bedi, G.S., Prep. Biochem. 22,67-81, 1992). The physico-chemical and immunological properties of three protei...

journal_title：Preparative biochemistry & biotechnology

authors： Bedi GS

更新日期：1996-05-01 00:00:00

abstract：:To isolate high-quality total RNA from Fallopia multiflora tuberous roots is difficult because of the presence of high levels of carbohydrates, phenolics, and other secondary metabolites. Since several procedures specialized for RNA isolation from polysaccharides and phenols rich tissues have resulted in poor yields, ...

journal_title：Preparative biochemistry & biotechnology

authors： Chen L,Sheng SJ,Tan XM,Shen YJ,Li HQ,Zhao SJ

更新日期：2012-01-01 00:00:00

abstract：:Microalgae are a promising feedstock for biofuel production. Lipid content in microalgae could be enhanced under nutrient depletion. This work investigated the effect of the nutrient on lipid accumulation in Ankistrodesmus sp. culture. Batch cultures were carried out using fresh BG11 medium, and after the harvest, the...

journal_title：Preparative biochemistry & biotechnology

authors： Sukkrom K,Bunnag B,Powtongsook S,Siangdung W,Pavasant P

更新日期：2016-07-03 00:00:00

abstract：:The microbial polysaccharides secreted and produced from various microbes into their extracellular environment is known as exopolysaccharide. These polysaccharides can be secreted from the microbes either in a soluble or insoluble form.Lactobacillus sp. is one of the organisms that have been found to produce exopolysa...

journal_title：Preparative biochemistry & biotechnology

authors： Suryawanshi N,Naik S,Eswari JS

更新日期：2019-01-01 00:00:00

abstract：:A microwave-assisted extraction (MAE) technology developed to extract polyphenols from the strawberry leaves was optimized by response surface methodology (RSM). Box-Behnken design (BBD) with four-factor and three-level was used to estimate the effects of extraction time, microwave power, ethanol concentration, and li...

journal_title：Preparative biochemistry & biotechnology

authors： Lin D,Ma Q,Zhang Y,Peng Z

更新日期：2020-01-01 00:00:00

abstract：:Proteases were identified and characterized from the culture supernatant of the C. diphtheriae and B. pertussis bacteria. The proteases were secreted in the media and detected at the end of the exponential growth phase. Activity was detected in some fluorescent substrates, based on selected protein sequences such as i...

journal_title：Preparative biochemistry & biotechnology

authors： Perpetuo EA,Lebrun I,Juliano L,Juliano MA,Sakauchi MA,Prado SM

更新日期：2007-01-01 00:00:00

abstract：:Protein fusion technology has emerged as one of the important strategies to increase the level of expression and half-life of therapeutic proteins in heterologous expression systems. Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor and is clinically used against neutropenia. Enhanced expr...

journal_title：Preparative biochemistry & biotechnology

authors： Sigar M,Maity N,Mishra S

更新日期：2017-04-21 00:00:00

abstract：:Members of the bacterial genus Acinetobacter have attracted great attention over the past few decades, on account of their various biotechnological applications and clinical implications. In this study, we are reporting the first experimental penicillin V acylase (PVA) activity from this genus. Penicillin acylases are...

journal_title：Preparative biochemistry & biotechnology

authors： Philem PD,Sonalkar VV,Dharne MS,Prabhune AA

更新日期：2016-07-03 00:00:00

abstract：:Enteropeptidase (EC 3.4.21.9) is the glycoprotein enzyme in the small intestine that triggers the activation of the zymogens in pancreatic juice by converting trypsinogen into trypsin. Because of its physiological significance, there have been many studies on the expression, purification, and characterization of enter...

journal_title：Preparative biochemistry & biotechnology

authors： Azhar M,Somashekhar R

更新日期：2015-01-01 00:00:00

abstract：:Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work, it was isolated into two forms of enzyme from yeast S. cerevisiae ce...

journal_title：Preparative biochemistry & biotechnology

authors： Margetić A,Vujčić Z

更新日期：2017-03-16 00:00:00

abstract：:Beta-glucuronidase (beta-Glu) and alkaline phosphatase (ALP) were evaluated in serum and urine in 50 subjects classified into five equal groups. Group I was control healthy subjects, while groups II, III, IV, and V were patients with nephritic syndrome, pyelonephritis, kidney rejection, and end-stage renal disease, re...

journal_title：Preparative biochemistry & biotechnology

authors： Refaie MO,Abo-Zaid H,Gomma NA,Aboul-Enein HY

更新日期：2000-05-01 00:00:00

abstract：:Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc. Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and c...

journal_title：Preparative biochemistry & biotechnology

authors： Jacob Kizhakedathil MP,Suvarna S,Belur PD,Wongsagonsup R,Agoo EMG,Janairo JIB

更新日期：2020-07-28 00:00:00

abstract：:Propionic acid production from glucose was studied using Propionibacterium freudenreichii shermanii. Conditions were optimized for high yields of propionic acid and total organic acids by sequential optimization of parameters like pH, inoculum age, inoculum volume and substrate concentration. Near-theoretical yield (0...

journal_title：Preparative biochemistry & biotechnology

authors： Pillai VV,Prakash G,Lali AM

更新日期：2018-01-02 00:00:00

abstract：:Fructose-1,6-bisphosphatase (FBPase), which is mainly used to supply NADPH, has an important role in increasing L-lysine production by Corynebacterium glutamicum. However, C. glutamicum FBPase is negatively regulated at the metabolic level. Strains that overexpressed Escherichia coli fructose-1,6-bisphosphatase in C. ...

journal_title：Preparative biochemistry & biotechnology

authors： Xu JZ,Zhang JL,Guo YF,Jia QD,Zhang WG

更新日期：2014-01-01 00:00:00

abstract：:The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg, protease type XVI, and trypsin, respectively. The pH dependence and incubation time were deter...

journal_title：Preparative biochemistry & biotechnology

authors： Doğru M,Baysal Z,Aytekin C,Aydin H

更新日期：2006-01-01 00:00:00

abstract：:The aims of the current study are to assess the influence of polyethylene glycol (PEG) concentration, molar mass, pH, and citrate concentrations on aqueous biphasic systems based on 24 factorial designs, as well as to check their capacity to purify tannase secreted by Aspergillus tamarii URM 7115. Tannase was produced...

journal_title：Preparative biochemistry & biotechnology

authors： de Sena AR,Barros Oliveira FM,Campos Leite TC,Evaristo da Silva Nascimento TC,Moreira KA,de Assis SA

更新日期：2017-10-21 00:00:00

abstract：:L-glutaminase from bacterial sources has been proven to be effective and economical agents in cancer therapy, food industry and high-value chemicals like threonine. In the present study, a newly isolated bacterial strain was potentially producing extracellular L-glutaminase, it identified as Bacillus subtilis OHEM11 (...

journal_title：Preparative biochemistry & biotechnology

authors： Orabi H,El-Fakharany E,Abdelkhalek E,Sidkey N

更新日期：2020-01-01 00:00:00

abstract：:Solid-state fermentation (SSF) is a bioprocess that doesn't need an excess of free water, and it offers potential benefits for microbial cultivation for bioprocesses and product development. In comparing the antibiotic production, few detailed reports could be found with lipolytic enzyme production by Streptomycetes i...

journal_title：Preparative biochemistry & biotechnology

authors： Cadirci BH,Yasa I,Kocyigit A

更新日期：2016-01-01 00:00:00