Abstract:
:Tissue acidosis is a common feature in many pathological conditions. Activation of acid-sensing ion channel 1a (ASIC1a) plays a key role in acidosis-mediated neurotoxicity. Protein kinase C (PKC) activity has been proved to be associated with many physiological processes and pathological conditions; however, whether PKC activation regulates ASIC1a protein expression and channel function remains ill defined. In this study, we demonstrated that treatment with phorbol 12-myristate 13-acetate (PMA, a PKC activator) for 6 h significantly increased ASIC1a protein expression and ASIC currents in NS20Y cells, a neuronal cell line, and in primary cultured mouse cortical neurons. In contrast, treatment with Calphostin C (a nonselective PKC inhibitor) for 6 h or longer decreased ASIC1a protein expression and ASIC currents. Similar to Calphostin C, PKC α and βI inhibitor Go6976 exposure also reduced ASIC1a protein expression. The reduction in ASIC1a protein expression by PKC inhibition involves a change in ASIC1a protein degradation, which is mediated by ubiquitin-proteasome system (UPS)-dependent degradation pathway. In addition, we showed that PKC regulation of ASIC1a protein expression involves NF-κB signaling pathway. Consistent with their effects on ASIC1a protein expression and channel function, PKC inhibition protected NS20Y cells against acidosis-induced cytotoxicity, while PKC activation potentiated acidosis-induced cells injury. Together, these results indicate that ASIC1a protein expression and channel function are closely regulated by the activity of protein kinase C and its downstream signaling pathway(s).
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
Zhang L,Leng TD,Yang T,Li J,Xiong ZGdoi
10.1007/s12035-020-02056-4subject
Has Abstractpub_date
2020-11-01 00:00:00pages
4754-4766issue
11eissn
0893-7648issn
1559-1182pii
10.1007/s12035-020-02056-4journal_volume
57pub_type
杂志文章abstract::It has been proposed that after nerve injury or tissue inflammation, fractalkine (CX3CL1) released from dorsal root ganglion neurons acts on satellite glial cells (SGCs) through CX3C receptor 1 (CX3CR1) to induce neuroplastic changes. The existence and importance of fractalkine/CX3CR1 signaling in the trigeminal gangl...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9935-x
更新日期:2017-07-01 00:00:00
abstract::Motilin is a 22-amino-acid gastrointestinal polypeptide that was first isolated from the porcine intestine. We identified that motilin receptor is highly expressed in GABAergic interneurons in the basolateral nucleus (BLA) of the amygdala, the structure of which is closely involved in assigning stress disorder and anx...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-012-8383-5
更新日期:2013-06-01 00:00:00
abstract::This study examined the association between elevated plasma homocysteine (Hcy) levels and the risk of acute cerebral infarction in patients with carotid artery lesions. A total of 78 patients were divided into two groups, the high Hcy group (n = 38; Hcy levels >15 umol/L) and the low Hcy group (n = 40; Hcy levels ≤15 ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9226-y
更新日期:2016-05-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT), a ribonucleoprotein, is reported as an important complex, which is required for stability of DNA molecular structure at the rear of the chromosome. Until now, hTERT has been linked to cell immortalization and tumorigenesis. A couple of articles have been published about ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,收录出版
doi:10.1007/s12035-015-9170-x
更新日期:2016-07-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disease which is characterized by progressive memory loss, the accumulation of β-amyloid peptide (Aβ) (mainly Aβ1-42), and more recently, by neuroinflammation, which has been highlighted as playing a central role in the development and progress of AD. This study utilized...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1211-9
更新日期:2019-04-01 00:00:00
abstract::Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder characterized by involuntary body movement, cognitive impairment and psychiatric disturbance. A polyglutamine expansion in the amino-terminal region of the huntingtin (htt) protein is the genetic cause of HD. Htt protein interacts with a wid...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-010-8153-1
更新日期:2011-02-01 00:00:00
abstract::Recent genome-wide association studies have identified two variants rs10033464 and rs2200733 on chromosome 4q25, significantly associated with ischemic stroke risk. We conducted this study to investigate whether these two variants were associated with age at onset and prognosis of ischemic stroke in a Chinese populati...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9903-5
更新日期:2017-07-01 00:00:00
abstract::The development of technologies for high-resolution nucleic acid localization in cells and tissues has contributed significantly to our understanding of transcriptional and translational regulation in eukaryotic cells. These methods include nonisotopic in situ hybridization methods for light and electron microscopy, a...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF02741301
更新日期:1998-12-01 00:00:00
abstract::Neuronal plasticity can be defined as adaptive changes in structure and function of the nervous system, an obvious example of which is the capacity to remember and learn. Long-term potentiation and long-term depression are the experimental models of memory in the central nervous system (CNS), and have been frequently ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF02802028
更新日期:1998-01-01 00:00:00
abstract::Retrograde signaling is defined as the signaling events leading from the plastids to the nucleus in plants and across the chemical synapse, from the postsynaptic neuron to the presynaptic neuron in animals. The discovery of various retrograde messengers has opened many avenues and clouds of thoughts as to the role of ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-015-9280-5
更新日期:2016-05-01 00:00:00
abstract::The complications caused by overweight, obesity and type 2 diabetes are one of the main problems that increase morbidity and mortality in developed countries. Hypothalamic metabolic sensors play an important role in the control of feeding and energy homeostasis. PAS kinase (PASK) is a nutrient sensor proposed as a reg...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-013-8630-4
更新日期:2014-10-01 00:00:00
abstract::PCDH19-Clustering Epilepsy (PCDH19-CE) is an infantile onset disorder caused by mutation of the X-linked PCDH19 gene. Intriguingly, heterozygous females are affected while hemizygous males are not. While there is compelling evidence that this disorder stems from the coexistence of WT and PCDH19-null cells, the cellula...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-020-02242-4
更新日期:2021-01-07 00:00:00
abstract::For the epigenetic characterization of neuromyelitis optica (NMO), we determined whether messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) are expressed differentially in subjects with and without NMO. lncRNA and mRNA expression profiles of NMO patients and healthy controls were generated by using microarray an...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9754-0
更新日期:2017-04-01 00:00:00
abstract::Social isolation during the vulnerable period of adolescence produces emotional dysregulation that often manifests as abnormal behavior in adulthood. The enduring consequence of isolation might be caused by a weakened ability to forget unpleasant memories. However, it remains unclear whether isolation affects unpleasa...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-014-8917-0
更新日期:2015-12-01 00:00:00
abstract::The vertebrate retina is a "genuine neural center" (Ramón y Cajal), in which glutamate is a major excitatory neurotransmitter. Both N-methyl-D-aspartate (NMDA) and non-NMDA receptors are expressed in the retina. Although non-NMDA receptors and/or metabotropic glutamate receptors are generally thought to be responsible...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1385/MN:34:3:163
更新日期:2006-12-01 00:00:00
abstract::Apoptosis plays a crucial role in the pathogenesis of early brain injury (EBI) following subarachnoid hemorrhage (SAH). However, the exact molecular mechanisms underlying neuronal apoptosis in EBI after SAH have not been fully elucidated. The present study showed that EBI induced significantly neuronal apoptosis activ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9490-x
更新日期:2016-10-01 00:00:00
abstract::α-Synuclein, an abundant and conserved presynaptic brain protein, is implicated as a critical factor in Parkinson's disease (PD). The aggregation of α-synuclein is believed to be a critical event in the disease process. α-Synuclein is characterized by a remarkable conformational plasticity, adopting different conforma...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-014-8818-2
更新日期:2015-01-01 00:00:00
abstract::Accumulating evidence suggests that inflammatory processes in the central nervous system that are mediated by microglial activation play important roles in several neurodegenerative disorders. Therefore, development of methods for microglial inhibition is considered an important strategy in the search for neuroprotect...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-013-8474-y
更新日期:2013-12-01 00:00:00
abstract::Iodine deficiency (ID) during development results in dysfunction of the central nervous system (CNS) and affects psychomotor and motor function. It is worth noting that maternal mild and marginal ID tends to be the most common reason of preventable neurodevelopmental impairment, via a mechanism that has not been eluci...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9382-0
更新日期:2016-09-01 00:00:00
abstract::Hypoxic-ischemic (HI) brain injury remains an important cause of brain damage in neonates with potential life-long consequences. Caffeine, which is a competitive inhibitor of adenosine receptors, is commonly used as treatment for preterm apnoea in clinical settings. In the current study, we investigated the effects of...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-020-01867-9
更新日期:2020-05-01 00:00:00
abstract::Quite a number of studies have examined the effects of fish oil supplementation on cognitive performance in different transgenic animal models of Alzheimer's disease (AD). However, inconsistent and controversial outcomes have been derived from these experiments. In order to investigate whether the beneficial effect of...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1060-6
更新日期:2018-12-01 00:00:00
abstract::OMI/HTRA2 (high-temperature requirement serine protease A2) is a mitochondrial serine protease involved in several cellular processes, including autophagy, chaperone activity, and apoptosis. Few studies on the role of OMI/HTRA2 in Alzheimer's disease (AD) are available, but none on its relationship with the cholinergi...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1383-3
更新日期:2019-07-01 00:00:00
abstract::Impaired glucose metabolism and mitochondrial decay greatly increase with age, when cognitive decline becomes rampant. No pharmacological or dietary intervention has proven effective, but proper diet and lifestyle do postpone the onset of neurodegeneration and some nutrients are being investigated. We studied insulin ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-0934-y
更新日期:2018-09-01 00:00:00
abstract::Vimentin is an intermediate filament (also known as nanofilament) protein expressed in several cell types of the central nervous system, including astrocytes and neural stem/progenitor cells. Mutation of the vimentin serine sites that are phosphorylated during mitosis (VIM SA/SA ) leads to cytokinetic failures in fibr...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-017-0759-0
更新日期:2018-07-01 00:00:00
abstract::The effects and mechanism of action of oligodeoxyribonucleotides containing CpG motif (CpG-ODNs) on neuron cells are largely unexamined. Here, we found that CpG-A ODNs but not other types of CpG-ODNs induced neurite retraction and cell apoptosis of rat embryonic neurons in a TLR9-independent manner. These effects of C...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-012-8275-8
更新日期:2012-06-01 00:00:00
abstract::Diabetic peripheral neuropathy (DPN) is a microvascular complication of diabetes mellitus. The aim of this meta-analysis was to evaluate the effects of methylenetetrahydrofolate reductase (MTHFR) 677 C>T and ACE I/D polymorphisms in the development of DPN. We systematically reviewed published studies on MTHFR 677 C>T ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,meta分析
doi:10.1007/s12035-016-9823-4
更新日期:2017-05-01 00:00:00
abstract::The human KCTD13 gene is located within the 16p11.2 locus and copy number variants of this locus are associated with a high risk for neuropsychiatric diseases including autism spectrum disorder and schizophrenia. Studies in zebrafish point to a role of KCTD13 in proliferation of neural precursor cells which may contri...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-019-01727-1
更新日期:2020-02-01 00:00:00
abstract::Statins have proven their effectiveness in the treatment of cardiovascular disease. This class of drugs has also attracted attention as a potential treatment for dissimilar diseases such as certain types of cancers and neurodegenerative diseases. What appears to be a contradiction is that, in the case of cancer, it ha...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-013-8496-5
更新日期:2013-10-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) have been shown to have useful properties for cell therapy and have been proposed for treatment of neurodegenerative diseases, including Parkinson's disease. However, the mechanisms involved in recovering dopaminergic neurons are not clear. The present study aims to evaluate the pathwa...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-017-0681-5
更新日期:2018-06-01 00:00:00
abstract::Many studies have shown that mitochondrial dysfunction and the subsequent oxidative stress caused by excessive reactive oxygen species (ROS) generation play a central role in the pathogenesis of Parkinson's disease (PD). We have previously shown that low-intensity ultrasound (LIUS) could reduce ROS generation by L-but...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-0104-z
更新日期:2017-10-01 00:00:00