Abstract：
:This investigation was carried out to identify the microbiota in a spoiled commercial food supplement consisting of a syrup suspension of a mixture of dried herbs and herb extracts. The product did not contain alkyl-p-hydroxybenzoates (parabens) as preservatives, was kept at room temperature and showed abundant gas formation. Colonies of distinct morphology were recovered on bacteria- and yeast-specific media, and tested for their ability to grow in the product. Genetic differentiation and identification of the microbial contaminants were achieved by RAPD-PCR and rDNA sequence analysis. The bacteria Bacillus megaterium, Bacillus subtilis, Paenibacillus humicus, Paenibacillus glycanilyticus, Staphylococcus aureus, Staphylococcus epidermidis and the yeasts Zygosaccharomyces rouxii and Zygosaccharomyces bailii were detected. Of the two S. aureus strains isolated, one was enterotoxigenic, as indicated by the presence of five SE genes. Quantitative Real-Time PCR tests, specific for this pathogen and for Z. bailii, a microbial agent causing fermentation processes and consequent food spoilage, were carried out to quantify these microorganisms in the product and identify their source among the herbal ingredients and the fructose syrup used as sweetener. Most components appeared to be contaminated by both S. aureus and Z. bailii. These findings indicate the need to improve hygienic practices in the industrial manufacturing of the food supplement, starting with herbal ingredients, to ensure a high quality of the product.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Rossi F,Gaio E,Torriani Sdoi
10.1016/j.fm.2009.11.011subject
Has Abstractpub_date
2010-05-01 00:00:00pages
356-62issue
3eissn
0740-0020issn
1095-9998pii
S0740-0020(09)00269-Xjournal_volume
27pub_type
杂志文章abstract：:Lactobacilli are Gram-positive and catalase negative rods commonly found in lactic acid fermented foods and in the gastrointestinal tract of mammals and birds. Few studies have described lactobacilli in freshwater fish. We analysed the presence of lactobacilli in the intestines of young and adult freshwater fish inhab...
journal_title：Food microbiology
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journal_title：Food microbiology
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journal_title：Food microbiology
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journal_title：Food microbiology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Food microbiology
pub_type： 杂志文章
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更新日期：2017-10-01 00:00:00
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journal_title：Food microbiology
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更新日期：2015-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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journal_title：Food microbiology
pub_type： 杂志文章
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journal_title：Food microbiology
pub_type： 杂志文章
doi：10.1016/j.fm.2008.03.002
更新日期：2008-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.fm.2008.04.013
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journal_title：Food microbiology
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更新日期：2008-10-01 00:00:00
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journal_title：Food microbiology
pub_type： 杂志文章
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