Abstract：
:The photoreceptor-specific tetraspanin glycoprotein RDS (retinal degeneration slow) is associated with many forms of inherited retinal disease. RDS shares features in common with other tetraspanin proteins, including the existence of a large intradiscal D2 loop containing several cysteines. While these cysteines are used only for intramolecular disulfide bonds in most tetraspanins, RDS expresses a seventh, unpaired cysteine (C150) used for intermolecular disulfide bonding in the formation of large RDS oligomers. To study oligomerization-dependent vs. oligomerization-independent RDS functions in rods, we generated a transgenic mouse line harboring a point mutation that replaces this Cys with Ser (C150S), leading to the expression of an RDS protein that cannot form intermolecular disulfide bonds. The mouse opsin promoter (MOP) was used to direct C150S RDS expression specifically in rods in these transgenic mice (MOP-T). Here we report improvement in scotopic ERGs in MOP-T/rds ( +/- ) mice (compared to non-transgenic rds ( +/- ) controls) and the appearance of malformed outer segments (OSs) in MOP-T mice that do not express native RDS (MOP-T/rds ( -/- )). These results suggest that while normal OS structure and function require RDS oligomerization, some RDS function is retained in the absence of C150. Since one of the functions of other tetraspanin proteins is to promote assembly of a membrane microdomain known as the "tetraspanin web", future studies may investigate whether assembly of this web is one of RDS's oligomerization-independent functions.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Chakraborty D,Conley SM,Fliesler SJ,Naash MIdoi
10.1007/978-1-4419-1399-9_5subject
Has Abstractpub_date
2010-01-01 00:00:00pages
39-46eissn
0065-2598issn
2214-8019journal_volume
664pub_type
杂志文章abstract：AIM:Hyperoxia-induced photoreceptor degeneration occurs preferentially in the inferior retina of C57BL/6 J mice. This study investigates differential gene expression in the inferior and superior retina of C57BL/6 J mouse, before and after hyperoxic stress. METHODS:At the age of P (postnatal day) 83-90, mice were place...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-1399-9_25
更新日期：2010-01-01 00:00:00
abstract：:The leukokinin-leukokininogen system is a pathological kinin generating system which is catalyzed by acid proteases present in neoplastic cells, white cells and even normal tissues. The components of the human system including leukokinin-H and leukokininogen have now been isolated and characterized. Very specific prot...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Deep learning is the state-of-the-art machine learning approach. The success of deep learning in many pattern recognition applications has brought excitement and high expectations that deep learning, or artificial intelligence (AI), can bring revolutionary changes in health care. Early studies of deep learning applied...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-33128-3_1
更新日期：2020-01-01 00:00:00
abstract：:Tumor growth is a highly complex, multistep process that involves tumor cell detachment, migration, invasion and metastasis accompanied by angiogenesis and extracellular matrix turn-over. Each of the steps is influenced by tumor cell interaction and interaction of the tumor cell with its microenvironment that consists...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-7913-1_9
更新日期：2010-01-01 00:00:00
abstract：:Overpopulation of selected groups of animals is widely recognised as an issue that can have adverse effects on several current global problems, such as animal and human health, conservation and environmental changes. This review will, therefore, focus on recent novel contraception together with future technologies tha...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4939-0820-2_18
更新日期：2014-01-01 00:00:00
abstract：:Serine/threonine kinase Akt is a downstream effector of insulin receptor/PI3K pathway that is involved in many processes, including providing neuroprotection to stressed rod photoreceptor cells. Akt signaling is known to be regulated by the serine/threonine phosphatases, PHLPP (PH domain and leucine rich repeat protei...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-17121-0_55
更新日期：2016-01-01 00:00:00
abstract：:The transparency of the eye can be disturbed by several eye diseases. It has recently been reported that periostin plays pivotal roles in the pathogenesis of several eye disease, such as diabetic retinopathy (DR), age-related macular degeneration (AMD), glaucoma, pterygia, corneal dystrophy, and chronic ocular allergi...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-13-6657-4_12
更新日期：2019-01-01 00:00:00
abstract：:A system for the extracorporeal treatment of end-stage liver failure patients has been developed and is now in clinical studies. This HepatAssist 2000 system consists of perfusing the plasma of the patient through a hollow-fiber bioreactor containing primary porcine hepatocytes. The goal of the system is to assist the...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_31
更新日期：1998-01-01 00:00:00
abstract：:Given recent progress in our understanding of oligodendrocyte biology, significant attention has been directed toward cell therapy for myelin repair and remyelination. This trend has been reinforced by findings about the importance of white matter lesions in a variety of central nervous system (CNS) diseases, includin...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-32-9636-7_17
更新日期：2019-01-01 00:00:00
abstract：:High-field (500 MHz) proton NMR has been used to elucidate the primary and secondary structures of glycosphingolipids (GSLs). Using 2-D J-correlated spectroscopy (2-D SECSY) which establishes scalar couplings of protons, the monosaccharide composition, anomeric configuration and aglycon structures of a GSL can be esta...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-1200-0_8
更新日期：1984-01-01 00:00:00
abstract：:Heparin-binding growth factors related to basic fibroblast growth factor are major determinants of the cellular clonal composition of adipose tissue. By providing and maintaining varying complements of preadipocytes in different fat depots, these factors contribute to the varying sizes and functions of different regio...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-2910-1_20
更新日期：1993-01-01 00:00:00
abstract：:Despite decades of research, cancer metastasis remains an incompletely understood process that is as complex as it is devastating. In recent years, there has been an increasing push to investigate the biomechanical aspects of tumorigenesis, complementing the research on genetic and biochemical changes. In contrast to ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4899-8032-8_20
更新日期：2014-01-01 00:00:00
abstract：:Many cases of West's syndrome are inoperable, because there is no clear epileptic focus. In some cases, however, there is a structural abnormality which can be surgically removed. Dr. Hoffman discusses his experience with surgery for cortical dysplasia in West's syndrome. ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:In the microenvironment of solid growing tumors, pronounced hypoxia or extracellular acidosis is commonly found. The aim of this study was the analysis of the cytotoxic effect of different chemotherapeutic agents (cisplatin, daunorubicin, docetaxel) under these conditions in vitro. Prostate carcinoma cells (R3327-AT1)...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-0-387-71764-7_21
更新日期：2007-01-01 00:00:00
abstract：:Preserving the integrity of proteins, biomolecules prone to molecular damage, is a fundamental function of all biological systems. Impairments in protein quality control (PQC) may lead to degenerative processes, such as aging and various disorders and diseases. Fortunately, cells contain a hierarchical system of pathw...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-7002-2_9
更新日期：2010-01-01 00:00:00
abstract：:Diisocyanate-induced asthma is difficult to diagnose since the immunopathological mechanisms and exposure determinants at the workplace are not well defined. The aim of this study was to evaluate the non-invasive methods of nasal lavage fluid (NALF) and induced sputum (IS) to enhance the diagnostic efficiency. Sixty-t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-94-007-6627-3_40
更新日期：2013-01-01 00:00:00
abstract：:Medical imaging now enables measurement of the lung in vivo at controlled volumes, prompting the development of increasingly sophisticated models of the geometry of the lung, from the largest airways and vessels to the alveolar tissue and capillary bed. We have developed methods for deriving subject-specific models of...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-0-387-73693-8_33
更新日期：2008-01-01 00:00:00
abstract：:The failure of complete remissions to reliably translate into cures in acute myeloid leukemia (AML) can be explained by the leukemia stem cell (LSC) paradigm, which hypothesizes that rare leukemia cells with stem cell features, including self-renewal capacity and drug resistance, are primarily responsible for both dis...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-14366-4_9
更新日期：2019-01-01 00:00:00
abstract：:Ninety-two institutionalized elderly subjects were vaccinated with trivalent influenza inactivated vaccine available for the 2011-2012 season, characterized by a prevalent circulation of A(H3N2) influenza viruses (A/Victoria/208-clade) presenting antigenic and genetic patterns different from the A(H3N2) vaccine compon...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2015_5003
更新日期：2016-01-01 00:00:00
abstract：:We report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess auto-ADP-ribosyltransferase activity and that Rt6, but not RT6, catalyzes the ADP-ribosylation of exogenous histones. Based on NH2OH sensitivity, it appeared that the ADP-ribose was attached to arginine residues on proteins. We also observed t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Receptor binding assays are a modern approach to the effects-related analysis of xenoendocrines. Human estrogen receptors are used to develop an analytical tool for the detection of estrogenic substances in environmental samples. A sensitive Enzyme Linked Receptor Assay (ELRA) was developed in a microwell plate format...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-0089-0_13
更新日期：1998-01-01 00:00:00
abstract：:Carefully tailored urinary diversion offers an excellent alternative in the spinal cord injured patient with chronic urine leakage and pressure sores following sphincterotomy. ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-8889-8_55
更新日期：2003-01-01 00:00:00
abstract：:This review examines the monooxygenase, peroxidase and peroxygenase properties and reaction mechanisms of cytochrome P450 (CYP) enzymes in bacterial, archaeal and mammalian systems. CYP enzymes catalyze monooxygenation reactions by inserting one oxygen atom from O2 into an enormous number and variety of substrates. Th...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-16009-2_1
更新日期：2015-01-01 00:00:00
abstract：:Lymphocyte differentiation from haematopoietic stem cells (HSCs) is a multi-step process in which lineage fate choices are made at crucial branch points. Plasticity of common precursors is evidenced by presence of transcriptionally favourable chromatin structures at several lineage-specific loci, making them poised fo...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-8216-2_3
更新日期：2011-01-01 00:00:00
abstract：KEY POINTS:The beginning of the twenty-first century offered new advances in cancer research, including the expansion of the knowledge about the tumor microenvironment (TME). Because TMEs provide the niches in which cancer cells, fibroblast, lymphocyte, and immune cells reside, they play a key role in cancer cell devel...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-77736-8_9
更新日期：2018-01-01 00:00:00
abstract：:Quantification of myocardial strains is essential for understanding cardiac mechanics. Previous techniques for assessing regional myocardial strains have been mainly limited to invasive procedures. A technique by which tagging can be added to magnetic resonance images (MRI) has recently been introduced and allows for ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-1893-8_31
更新日期：1995-01-01 00:00:00
abstract：:Rats fed a high-fat diet before and during lactation have difficulty initiating lactation and have high pup mortality rates, low milk production and, consequently, poor pup growth. To determine if these adverse outcomes can be mitigated with dietary changes made after delivery, obese Sprague-Dawley rats (who had previ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-1371-1_12
更新日期：2001-01-01 00:00:00
abstract：:We have described our current criteria for selecting those patients with acute V T-E who merit long-term protection, for accomplishing the acute to long-term transition efficiently and for deciding when such protection can be interrupted. Naturally, in individual patients, special circumstances may exist which alter t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4757-5985-3_17
更新日期：1987-01-01 00:00:00
abstract：:The survival of three-dimensional tissue requires a vascular network to provide transport of oxygen and metabolic byproduct. Here, we report a new approach to create capillary blood vessels in vitro on biomaterials suitable for use as scaffolds in engineering tissues. Endothelial cells were cultured on chemical and to...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-0-387-74911-2_23
更新日期：2008-01-01 00:00:00
abstract：:Kallikreins were localized in the human parotid gland and in the human kidney from two different aspects of the enzyme: the enzyme antigenicity and the enzyme activity. In the human parotid gland, the kallikrein antigenicity and the enzyme activity were identical in their locations in the ductal cells. In the human ki...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5143-6_4
更新日期：1986-01-01 00:00:00
