Detection of Leishmania braziliensis DNA in American tegumentary leishmaniasis patients.

Abstract:

:Diagnostic tests for American tegumentary leishmaniasis were performed on blood samples of patients living in two endemic municipalities in the state of Pernambuco, Northeastern Brazil. DNA was extracted from 119 samples and used as template for polymerase chain reaction (PCR) analysis. The tests used primers specific for the kinetoplast mini-circle DNA (kDNA) of Leishmania braziliensis, a species circulating in Pernambuco, which amplify a 750 base pair target sequence. In total, 58 subjects (48.7%) showed positive PCR amplification and 61 (51.3%) were negative. Of the PCR-positive samples, 37 ( congruent with 64%) were from treated, lesion-free subjects. In conclusion, the PCR technique is efficacious at identifying Leishmania DNA in biopsy and venous blood samples.

journal_name

Rev Saude Publica

journal_title

Revista de saude publica

authors

Martins L,Alexandrino A,Guimarães G

doi

10.1590/s0034-89102010005000004

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

571-4

issue

3

eissn

0034-8910

issn

1518-8787

pii

S0034-89102010005000004

journal_volume

44

pub_type

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