Abstract：
:Monogenic diseases are often severe, life-threatening disorders for which lifelong palliative treatment is the only option. Over the last two decades, a number of strategies have been devised with the aim to treat these diseases with a genetic approach. Gene therapy has been under development for many years, yet suffers from the lack of an effective and safe vector for the delivery of genetic material into cells. More recently, gene targeting by homologous recombination has been proposed as a safer treatment, by specifically correcting disease-causing mutations. However, low efficiency is a major drawback. The emergence of two technologies could overcome some of these obstacles. Terminally differentiated somatic cells can be reprogrammed, using defined factors, to become induced pluripotent stem cells (iPSCs), which can undergo efficient gene mutation correction with the aid of fusion proteins known as zinc finger nucleases (ZFNs). The amalgamation of these two technologies has the potential to break through the current bottleneck in gene therapy and gene targeting.
journal_name
Biotechnol Advjournal_title
Biotechnology advancesauthors
Wong GK,Chiu ATdoi
10.1016/j.biotechadv.2010.05.019subject
Has Abstractpub_date
2010-11-01 00:00:00pages
715-24issue
6eissn
0734-9750issn
1873-1899pii
S0734-9750(10)00070-4journal_volume
28pub_type
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2010.08.007
更新日期：2010-11-01 00:00:00
abstract：:Citric acid is the principal organic acid found in citrus fruits. To meet increasing demands it is produced from carbohydrate feedstock by fermentation with the fungus Aspergillus niger and the yeasts of Candida spp. Effect of various fermentation conditions and the biochemistry of citric acid formation by A. niger ha...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(95)00002-8
更新日期：1995-01-01 00:00:00
abstract：:The population of sub-Saharan Africa is at risk from multiple, poverty-related endemic diseases. HIV and malaria are the most prevalent, but they disproportionately affect different groups of people, i.e. HIV predominantly affects sexually-active adults whereas malaria has a greater impact on children and pregnant wom...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2014.02.004
更新日期：2014-05-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.07.002
更新日期：2015-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2011.03.005
更新日期：2011-07-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2009.07.002
更新日期：2009-11-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.02.004
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.08.001
更新日期：2015-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(85)90292-7
更新日期：1985-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.03.007
更新日期：2018-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.12.001
更新日期：2013-03-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.08.019
更新日期：2014-01-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/0734-9750(93)90016-g
更新日期：1993-01-01 00:00:00
abstract：:Compared with the better-studied canonical amino acids, the distribution, metabolism and functions of natural non-canonical amino acids remain relatively obscure. Natural non-canonical amino acids have been mainly discovered in plants as secondary metabolites that perform diversified physiological functions. Due to th...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.07.008
更新日期：2018-11-15 00:00:00
abstract：:Plant oil based industrial oleochemistry leads to a large side stream of crude glycerol, which offers itself as a low price carbon source for microbial chemical production. Compared to sugar, glycerol is more reduced and less microorganisms are able to use it as carbon source. An interesting feature of glycerol conver...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2019.05.001
更新日期：2019-11-01 00:00:00
abstract：:The concept of the Truncated Light-harvesting chlorophyll Antenna (TLA) size, as a tool by which to maximize sunlight utilization and photosynthetic productivity in microalgal mass cultures or high-density plant canopies, is discussed. TLA technology is known to improve sunlight-to-product energy conversion efficienci...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.08.018
更新日期：2014-01-01 00:00:00
abstract：:Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expres...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.05.008
更新日期：2015-11-01 00:00:00
abstract：:Volatile organic compounds (VOCs) emitted to the environment highly probably result in ecological and health risks. Many biotechnologies for waste gases containing hydrophobic VOCs have been developed in recent years. However, these biological processes usually exhibit poor removal performances for hydrophobic VOCs du...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.06.007
更新日期：2016-11-01 00:00:00
abstract：:Iron is indispensible for life and essential for such processes as oxygen transport, electron transfer and DNA synthesis. Transferrin (Tf) is a ubiquitous protein with a central role in iron transport and metabolism. There is evidence, however, that Tf has many other biological roles in addition to its primary functio...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2010.11.007
更新日期：2011-03-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.05.010
更新日期：2016-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2020.107635
更新日期：2020-12-01 00:00:00
abstract：:Yeast strains Y1, Y4 and Y7 demonstrated high conversion efficiencies for sugars and high abilities to tolerate or metabolize inhibitors in dilute-acid lignocellulosic hydrolysates. Strains Y1 and Y4 completely consumed the glucose within 24 h in dilute-acid lignocellulosic hydrolysate during in situ detoxification, a...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/j.biotechadv.2009.04.008
更新日期：2009-09-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2015.10.013
更新日期：2015-12-01 00:00:00
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journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2012.07.003
更新日期：2012-11-01 00:00:00
abstract：:The global biopharmaceutical market is worth over $100 billion USD. Nearly 90% of these products will lose their patent in the next ten years, leading to the commercialization of their subsequent versions, known as 'biosimilars'. Biosimilars are much more complex molecules than chemically synthesized generics in terms...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2013.05.005
更新日期：2013-12-01 00:00:00
abstract：:Human immunodeficiency virus (HIV) remains a worldwide health problem despite huge investments and research breakthroughs, and no single drug is effective in killing the virus yet. Among new strategies to control HIV infection, the phage display (PD) technology has become a promising tool in the discovery of peptides ...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2018.07.003
更新日期：2018-11-15 00:00:00
abstract：:Lignin is a major component of lignocellulosic biomass and as such, it is processed in enormous amounts in the pulp and paper industry worldwide. In such industry it mainly serves the purpose of a fuel to provide process steam and electricity, and to a minor extent to provide low grade heat for external purposes. Also...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:Small molecule metabolites secreted by pathological processes can act as molecular biomarkers for clinical diagnosis. In vitro detection of the metabolites such as glucose and reactive oxygen species is of great significance for precise screening, monitoring and prognosis of metabolic disorders and relevant diseases s...
journal_title：Biotechnology advances
pub_type： 杂志文章,评审
doi：10.1016/j.biotechadv.2016.01.006
更新日期：2016-05-01 00:00:00
abstract：:Bovine chymosin, an aspartyl protease extracted from abomasum of suckling calves, is synthesized in vivo as preprochymosin and secreted as prochymosin which is autocatalytically activated to chymosin. Chymosin is bilobular, with Asp 32 and Asp 215 acting as the catalytic residues. Chymosin A and chymosin B have pH opt...
journal_title：Biotechnology advances
pub_type： 杂志文章
doi：10.1016/s0734-9750(99)00010-5
更新日期：1999-09-01 00:00:00
