Experimental varicocele induces p53-dependent germ cell apoptosis through activation of γ-H2AX.

Abstract:

AIM:Since DNA damage-related apoptosis is raising concerns regarding abnormal spermatogenesis, we investigated the changes in γ-H2AX during testicular germ cell apoptotic responses in the varicocele model. MATERIALS AND METHODS:Varicocele was induced by partial ligation of the left renal vein and animals were sacrificed at 1, 3, and 4 weeks after varicocele creation. The levels of activated p53 and γ-H2AX formation were determined by Western blot analysis and immunohistochemistry. RESULTS:γ-H2AX formation was augmented after varicocele creation, while a significant increase in p53 phosphorylation was detected in a time course-dependent manner. Varicocele-dependent nuclear γ-H2AX staining in the primary spermatocytes was prominent as degenerative foci, while little differences could be detected in spermatogonia. CONCLUSIONS:These results show that experimental varicocele may induce p53-dependent apoptosis through activation of γ-H2AX in the primary spermatocytes, and suggest that γ-H2AX may be related to apoptotic signal transduction in experimental varicocele.

journal_name

Urol Int

journal_title

Urologia internationalis

authors

Chang IY,Kim JH,Park KH,Yoon SP

doi

10.1159/000316356

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

216-20

issue

2

eissn

0042-1138

issn

1423-0399

pii

000316356

journal_volume

85

pub_type

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