Abstract:
:This paper describes an investigation of the use of poly(lactic/glycolic acid) polymers for long-term delivery of high molecular weight, water-soluble proteins. Poly(lactic/glycolic acid) (PLGA) microspheres, containing (fluorescein isothiocyanate)-labeled bovine serum albumin and (fluorescein isothiocyanate)-labeled horseradish peroxidase, were prepared by a modified solvent evaporation method using a double emulsion. The microspheres were spherical with diameters of 55-95 microns and encapsulated more than 90% of the protein. The preparation method was gentle and maintained enzyme activity and protein solubility. Stability studies showed that the encapsulation of an enzyme inside PLGA microspheres can protect them from activity loss. When not placed inside PLGA microspheres, (fluorescein isothiocyanate)-labeled horseradish peroxidase lost 80% of its activity in solution at 37 degrees C in a few days, whereas inside the PLGA microspheres it retained more than 55% of its activity after 21 days of incubation at 37 degrees C. In vitro release studies revealed that different release profiles (i.e., near-constant or biphasic) and release rates can be achieved by simply modifying factors in the preparation procedure such as mixing rate and volume of inner water and organic phases. Degradation studies by scanning electron microscopy and gel-permeation chromatography suggested that the mechanism responsible for protein release is mainly through matrix erosion.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Cohen S,Yoshioka T,Lucarelli M,Hwang LH,Langer Rdoi
10.1023/a:1015841715384subject
Has Abstractpub_date
1991-06-01 00:00:00pages
713-20issue
6eissn
0724-8741issn
1573-904Xjournal_volume
8pub_type
杂志文章abstract::Growing tumors develop additional new blood vessels to meet the demand for adequate nutrients and oxygen, a process called angiogenesis. Cancer is a highly complex disease promoted by excess angiogenesis; interfering with this process poses for an attractive approach for controlling tumor growth. This hypothesis led t...
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journal_title:Pharmaceutical research
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更新日期:2015-08-01 00:00:00
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更新日期:1993-02-01 00:00:00
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更新日期:2000-06-01 00:00:00
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更新日期:1999-08-01 00:00:00
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更新日期:2018-11-07 00:00:00
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