Abstract:
:Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used as universal genetically encoded fluorescent labels. Many laboratories have focused their efforts on identification and development of fluorescent proteins with novel characteristics and enhanced properties, resulting in a powerful toolkit for visualization of structural organization and dynamic processes in living cells and organisms. The diversity of currently available fluorescent proteins covers nearly the entire visible spectrum, providing numerous alternative possibilities for multicolor labeling and studies of protein interactions. Photoactivatable fluorescent proteins enable tracking of photolabeled molecules and cells in space and time and can also be used for super-resolution imaging. Genetically encoded sensors make it possible to monitor the activity of enzymes and the concentrations of various analytes. Fast-maturing fluorescent proteins, cell clocks, and timers further expand the options for real time studies in living tissues. Here we focus on the structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging, with particular attention to recent techniques.
journal_name
Physiol Revjournal_title
Physiological reviewsauthors
Chudakov DM,Matz MV,Lukyanov S,Lukyanov KAdoi
10.1152/physrev.00038.2009subject
Has Abstractpub_date
2010-07-01 00:00:00pages
1103-63issue
3eissn
0031-9333issn
1522-1210pii
90/3/1103journal_volume
90pub_type
杂志文章,评审abstract::The 1910-1913 Terra Nova Expedition to the Antarctic, led by Captain Robert Falcon Scott, was a venture of science and discovery. It is also a well-known story of heroism and tragedy since his quest to reach the South Pole and conduct research en route, while successful was also fateful. Although Scott and his four co...
journal_title:Physiological reviews
pub_type: 传,历史文章,杂志文章
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2000-07-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2005-07-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:1990-10-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2004-07-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:1985-10-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
doi:10.1152/physrev.00023.2001
更新日期:2002-01-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
doi:10.1152/physrev.1996.76.2.319
更新日期:1996-04-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
doi:10.1152/physrev.00037.2009
更新日期:2010-04-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2016-07-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2006-01-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2003-07-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2006-10-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
doi:10.1152/physrev.2001.81.4.1689
更新日期:2001-10-01 00:00:00
abstract::Astrocytes are the most abundant cells in the central nervous system (CNS) that provide nutrients, recycle neurotransmitters, as well as fulfill a wide range of other homeostasis maintaining functions. During the past two decades, astrocytes emerged also as increasingly important regulators of neuronal functions inclu...
journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2014-10-01 00:00:00
abstract::Peripheral mediators can contribute to the development and maintenance of inflammatory and neuropathic pain and its concomitants (hyperalgesia and allodynia) via two mechanisms. Activation or excitation by these substances of nociceptive nerve endings or fibers implicates generation of action potentials which then tra...
journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2012-10-01 00:00:00
abstract::Given the large amount of genome-wide data that have been collected during the last decades, a good understanding of how and why cells change during development, homeostasis, and disease might be expected. Unfortunately, the opposite is true; triggers that cause cellular state changes remain elusive, and the underlyin...
journal_title:Physiological reviews
pub_type: 杂志文章
doi:10.1152/physrev.00034.2019
更新日期:2021-01-01 00:00:00
abstract::The Arp2/3 complex is an evolutionary conserved molecular machine that generates branched actin networks. When activated, the Arp2/3 complex contributes the actin branched junction and thus cross-links the polymerizing actin filaments in a network that exerts a pushing force. The different activators initiate branched...
journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
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journal_title:Physiological reviews
pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
abstract::Animals synchronize to the environmental day-night cycle by means of an internal circadian clock in the brain. In mammals, this timekeeping mechanism is housed in the suprachiasmatic nucleus (SCN) of the hypothalamus and is entrained by light input from the retina. One output of the SCN is a neural code for circadian ...
journal_title:Physiological reviews
pub_type: 杂志文章,评审
doi:10.1152/physrev.00027.2019
更新日期:2020-10-01 00:00:00