Abstract：
Objective:The objective was to investigate the expression of macrophage migration inhibitory factor (MIF) in non-small cell lung cancer (NSCLC), as well as the effects of macrophage MIF on tumor cells. Methods:The human NSCLC cell strains H358 and H524 were selected as research objects. The Real-Time Polymerase Chain Reaction (RT-PCR) and Western Blot were utilized to detect the expression levels of MIF in human NSCLC cell strains. The lentiviral plasmid was utilized for MIF-mRNA interference. The expression levels of MIF before and after transfection were compared. The cell strains were cultured and proliferated for cell count and comparison. Results:H358 showed MIF high expression while H524 showed MIF low expression. Once the H358 cells were constructed as silent MIF expression, compared with the original H358 cells, the difference was statistically significant. Once the H524 cells were constructed as high MIF expression, compared with original H524 cells, the difference was statistically significant. Being cultured for respective 3, 5, and 7 days, the transfected H358 cells showed a significant decrease in proliferative activity compared with original H358 cells, while the transfected H524 cells showed a significant increase in proliferative activity compared with original H524 cells. Conclusion:MIF has high expression in H358 cells while low expression in H524 cells. The expression of MIF could enhance the proliferative activity of NSCLC tumor cells.
journal_name
Saudi J Biol Scijournal_title
Saudi journal of biological sciencesauthors
Zhang H,Duan J,Wu Odoi
10.1016/j.sjbs.2020.04.027subject
Has Abstractpub_date
2020-06-01 00:00:00pages
1527-1532issue
6eissn
1319-562Xissn
2213-7106pii
S1319-562X(20)30140-6journal_volume
27pub_type
杂志文章abstract：:Rhazya stricta Decne is an important medicinal species used in indigenous medicinal herbal drugs to cure various diseases in South Asia (Pakistan, India and Afghanistan) and in the Middle East (e.g. Saudi Arabia, Qatar, United Arab Emirates (UAE), Iran and Iraq). Some of its alkaloids have been reported to have antica...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.10.017
更新日期：2017-11-01 00:00:00
abstract：:Legumes are essential and play a significant role in maintaining food standards and augmenting physiochemical soil properties through the biological nitrogen fixation process. Biotic and abiotic factors are the main factors limiting legume production. Classical breeding methodologies have been explored extensively abo...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2019.11.018
更新日期：2020-01-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is the most commom cancer in the world. If remain untreated for several years, it may be fatal. Hence, it is important to prevent and treat OSCC at an early stage. In this study the effect of aqueous and dry leaves extract of Ocimum sanctum was observed on Ca9-22 cell line, which is...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.11.030
更新日期：2021-01-01 00:00:00
abstract：:Gelatin is used as an ingredient in both food and non-food industries as a gelling agent, stabilizer, thickener, emulsifier, and film former. Porcine skins, bovine hides, and cattle bones are the most common sources of gelatin. However, mammalian gelatins are rejected by some consumers due to social, cultural, religio...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.03.022
更新日期：2020-06-01 00:00:00
abstract：:Teucrium (Lamiaceae) is a large and polymorphic genus distributed mainly in Europe, North Africa and in the temperate parts of Asia. In this study, the anatomical features of the leaf and stem of Teucrium polium are investigated. Teucrium has 19 taxa in Iran, which mainly grow in the Irano-Turanian region between 700 ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2014.11.002
更新日期：2015-03-01 00:00:00
abstract：:A level of water quality intended for human consumption does not seem necessary for domestic uses such as irrigation of green spaces. Alternative water supplies like the use of greywater (GW) can thus be considered. However, GW contains pathogenic microorganisms and organic compounds which can cause environmental and ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.01.006
更新日期：2019-01-01 00:00:00
abstract：:Ubiquitin expression protein DNA clone (Hl-UBI) was isolated from Heterodera latipons collected from North Jordan. Its sequence of 285 nucleotides was also determined and deposited in the GeneBank. The 285-bp open reading frame coded for 76-amino acid protein having two domains; the ubiquitin domain and the C terminal...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.06.005
更新日期：2019-11-01 00:00:00
abstract：:Partial root-zone drying during irrigation (PRD) has been shown effective in enhancing plant water use efficiency (WUE), however, the roles of chemical signals from root and shoot that are involved and the possible interactions affected by nitrogen nutrition are not clear. Pot-grown cotton (Gossypium spp.) seedlings w...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.01.015
更新日期：2017-03-01 00:00:00
abstract：:Stimulation of complex chaperone activity may be a viable means of therapy for neurodegenerative diseases. These chaperons execute reactivation of thermally and chemically aggregated protein substrates by cooperating with their partner co-chaperons. We optimized the expression and purification conditions of Tid1-L cha...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.09.006
更新日期：2020-11-01 00:00:00
abstract：:This study aimed to estimate the proximate, phenolic and flavonoids contents and phytochemicals present in seeds of twenty four soybeans (Glycine max (L.) Merr) genotypes to explore their nutritional and medicinal values. Crude protein composition ranged between 35.63 and 43.13% in Argentinian and USA (Clark) genotype...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.10.014
更新日期：2018-01-01 00:00:00
abstract：:Oat (Avena sativa L.) is an important fodder crop of Pakistan, though with low productivity. The present study was conducted to evaluate the performance and genetic diversity of exotic oat germplasm, with emphasis on cereal yellow dwarf virus resistance. A total of 16 exotic line (introduced from Aarhus University Den...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.05.042
更新日期：2020-10-01 00:00:00
abstract：:Both leica microscopic camera system and scanning electron microscopy was used to observe and characterize the feet, back, abdomen, antennae and mouthparts of the Pseudoregma bambucicola from the bamboo, Bambusa multiplex. The possible functions of all the external morphological characteristics of the P. bambucicola w...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.05.007
更新日期：2017-11-01 00:00:00
abstract：:Corona viruses (CoV) are known to cause extreme pandemics in the globe. The year 2020 will be a pandemic with the spread of the novel coronavirus (SARS-CoV-2) across the globe. Coronavirus 2019 (COVID-19) has been a part of our scary life for more than a quarter of a year in 2020. The Wuhan market and China have been ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.11.035
更新日期：2021-01-01 00:00:00
abstract：:Fish kidneys are sensitive to chemical changes in the freshwater ecosystem because they are directly and constantly exposed to chemicals dissolved in the water. This study evaluated nephrotoxicity in Wallago attu and Cirrhinus mrigala harvested from the Chenab River in an area of industrial and sewage waste disposal. ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.02.003
更新日期：2019-05-01 00:00:00
abstract：:In the present study, application of Ecklonia maxima extract (Kelpak SL - a water soluble concentrate) was optimized and its impact on yield, nutraceutical and nutritional potential of Phaseolus vulgaris L. (var. Aura and Toska) was measured. The study was carried out in 2012 and 2013 in Poland. During the growing sea...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2016.01.039
更新日期：2018-03-01 00:00:00
abstract：:Based on the massive production of cow and poultry manures, farmers in Saudi Arabia are moving towards the application of organic fertilizers in their farms. Therefore, the present work was conducted to study the response of soil and selected vegetable crops to poultry and cow manures, using ground data and Landsat-8 ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.06.006
更新日期：2019-11-01 00:00:00
abstract：:Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the p...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：:The present study aimed to investigate the contents of glucosinolates (GSLs) and carotenoids in eleven varieties of Chinese cabbage in relation to the expression level of the important transcription factors. MS and HPLC analysis identified the presence of 13 GSLs (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin,...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2016.04.004
更新日期：2018-01-01 00:00:00
abstract：:The effects of exposing meat-type breeder eggs to magnetic field (MF) before incubation on hatchability traits (percents of hatchability and hatchability failures of eggs), chick weight at hatch, and post-hatch performance (weight gain, feed intake, and feed conversion ratio (FCR)) from 1 to 39 d of age were investiga...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2011.06.004
更新日期：2011-10-01 00:00:00
abstract：:Cadmium is among the toxic and hazardous metal widely dispersed in the environment in high levels. Current studies have provided new insights into antioxidant properties of bioflavonoid which have emerged as probable therapeutic and nutraceutical agents. The present study is geared to investigate the possible role of ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.01.002
更新日期：2019-11-01 00:00:00
abstract：:In forensic science, the human identification is the major goal in criminal cases and in paternity dispute, based on DNA profiling of individual. Analysis of short tandem repeat (STRs) markers used as a reliable technique for this purpose. In this study the main objective was to develop a human identification based on...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：:In spite of availability of several plastomes representing different tribes of Plantaginaceae, sparse attempts have been made to understand the plastome structure, evolution, and phylogenomics. In the present study, we have made an effort to understand the gene content and plastome evolution in the family Plantaginace...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.09.040
更新日期：2020-12-01 00:00:00
abstract：:Metabolic syndrome (Mets) is a major health hazard. The syndrome is strongly linked to cardiovascular disease. The objective of the current study was to address whether or not crocin could protect against experimentally-induced MetS in rats as well as the possible underlying mechanisms. Animals were allocated into 4 g...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.01.004
更新日期：2020-05-01 00:00:00
abstract：:The intention of the present wok is to provide an account on the pollen morphological features of Lamiaceae in Saudi Arabia as a basis for future studies of Lamiaceae pollens in the region. Pollen morphology of 20 species belong to 16 genera of the Lamiaceae has been investigated using Light Microscope (LM) and Scanni...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.03.001
更新日期：2018-02-01 00:00:00
abstract：:Regular exposure to stress causes alteration in biochemical parameter but till date no specific medicine prescribed for controlling it. Current study aimed to determine the effect of Diazepam on proinflammatory and cardiac markers in stress exposed rats. Male Wistar rats were divided into four groups with six animals ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.10.042
更新日期：2021-01-01 00:00:00
abstract：:Tomato (Lycopersicon esculentum) is one of the widely grown vegetables worldwide. Fusarium oxysporum f. sp. lycopersici (FOL) is the significant contributory pathogen of tomato vascular wilt. The initial symptoms of the disease appear in the lower leaves gradually, trail by wilting of the plants. It has been reported ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2019.06.002
更新日期：2019-11-01 00:00:00
abstract：:Antibiotic resistance is a problem of deep scientific concern both in hospital and community settings. Rapid detection in clinical laboratories is essential for the judicious recognition of antimicrobial resistant organisms. Production of extended-spectrum β-lactamases (ESBLs) is a significant resistance-mechanism tha...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2014.08.002
更新日期：2015-01-01 00:00:00
abstract：:Bamboo macromolecules were pretreated with bamboo vinegar, which has an antibacterial property, and processed into antibacterial bioboard (ABB) by hot pressing. The ABB was then analyzed by conducting Fourier-transform infrared spectroscopy, thermogravimetric analysis and differential thermal analysis. Results showed ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.08.010
更新日期：2018-03-01 00:00:00
abstract：:Land-use intensification, contrary to sustainable land management, has an impact on the healthiness of the environmental agroecosystem. To assess the environmental implications in abandoned land, olive groves and maize crops, the most sensitive and reliable edaphic indicators were measured to estimate plant species di...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.08.012
更新日期：2020-12-01 00:00:00
abstract：:Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among the Gram negative bacteria, P. mirabilis is the dominant biofilm producer in urinary tract infections next to E. coli. Biofilm is a process that produced self-matrix of more virulen...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.10.003
更新日期：2021-01-01 00:00:00
