Abstract:
RATIONALE:Serotonin and especially serotonin 2A (5-HT(2A)) receptor signaling are important in the etiology and treatment of schizophrenia and affective disorders. We previously reported a novel 5-HT(2A) receptor effector, increased transglutaminase (TGase)-catalyzed transamidation, and activation of the small G protein Rac1 in A1A1v cells, a rat embryonic cortical cell line. OBJECTIVES:In this study, we explore the signaling pathway involved in 5-HT(2A) receptor-mediated Rac1 transamidation. METHODS:A1A1v cells were pretreated with pharmacological inhibitors of phospholipase C (PLC) or calmodulin (CaM), and then stimulated by the 5-HT(2A) receptor agonist, 2,5-dimethoxy-4-iodoamphetamine (DOI). Intracellular Ca(2+) concentration and TGase-modified Rac1 transamidation were monitored. The effect of manipulation of intracellular Ca(2+) by a Ca(2+) ionophore or a chelating agent on Rac1 transamidation was also evaluated. RESULTS:In cells pretreated with a PLC inhibitor U73122, DOI-stimulated increases in the intracellular Ca(2+) concentration and TGase-modified Rac1 were significantly attenuated as compared to those pretreated with U73343, an inactive analog. The membrane-permeant Ca(2+) chelator, BAPTA-AM strongly reduced TGase-catalyzed Rac1 transamidation upon DOI stimulation. Conversely, the Ca(2+) ionophore ionomycin, at a concentration that induced an elevation of cytosolic Ca(2+) to a level comparable to cells treated with DOI, produced an increase in TGase-modified Rac1 without 5-HT(2A) receptor activation. Moreover, the CaM inhibitor W-7, significantly decreased Rac1 transamidation in a dose-dependent manner in DOI-treated cells. CONCLUSIONS:These results indicate that 5-HT(2A) receptor-coupled PLC activation and subsequent Ca(2+) and CaM signaling are necessary for TGase-catalyzed Rac1 transamidation, and an increase in intracellular Ca(2+) is sufficient to induce Rac1 transamidation.
journal_name
Psychopharmacology (Berl)journal_title
Psychopharmacologyauthors
Dai Y,Dudek NL,Li Q,Muma NAdoi
10.1007/s00213-010-1984-7subject
Has Abstractpub_date
2011-02-01 00:00:00pages
403-12issue
2-3eissn
0033-3158issn
1432-2072journal_volume
213pub_type
杂志文章abstract::The effects of meperidine on operant behavioral thermoregulation were investigated using a convective thermal controller and mice trained to alternate at will the thermal-drive condition, changing it from an air flow of 15 degrees C to one of 45 degrees C, and vice versa. Administration of 15 mg/kg meperidine resulted...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00491979
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND:This laboratory study investigated the mechanisms by which the opioid antagonist, naltrexone, reduces the risk of relapse to heavy drinking in individuals with alcohol dependence. METHODS:Eighteen alcohol-dependent, non-treatment-seeking volunteers were randomized to 50 mg naltrexone or placebo for 6 days a...
journal_title:Psychopharmacology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s002130100919
更新日期:2002-02-01 00:00:00
abstract:RATIONALE:Morphine and buprenorphine have analgesic and anxiolytic-like properties. While their analgesic effects have been well characterized, their anxiolytic-like properties have not. OBJECTIVES:Effects of acute morphine and buprenorphine on the expression of acoustic fear-potentiated startle (FPS) and naloxone pre...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-008-1112-0
更新日期:2008-06-01 00:00:00
abstract:RATIONALE:Nicotine can enhance attentional performance in humans, a property that may be of therapeutic utility. OBJECTIVES:To identify brain sites mediating nicotine-induced attentional enhancement. METHODS:Nicotine (0, 1, 2, 4 and 8 microg) was injected bilaterally into the dorsal hippocampus and the prelimbic area...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-003-1438-6
更新日期:2003-07-01 00:00:00
abstract:RATIONALE:Buprenorphine reduces both heroin and cocaine intake in opioid addicts, but the mechanisms remain unclear. OBJECTIVES:To determine the effects of chronic buprenorphine treatment on intake of heroin and/or cocaine and measure nucleus accumbens (NAc) dopamine (DA) levels during self-administration. METHODS:In...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-006-0485-1
更新日期:2006-09-01 00:00:00
abstract:RATIONALE:Pairing rewarding outcomes with audiovisual cues in simulated gambling games increases risky choice in both humans and rats. However, the cognitive mechanism through which this sensory enhancement biases decision-making is unknown. OBJECTIVES:To assess the computational mechanisms that promote risky choice d...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-019-05308-x
更新日期:2019-08-01 00:00:00
abstract:RATIONALE:Available neurochemical probes that lower brain dopamine (DA) levels in man are limited by their tolerability and efficacy. For instance, the acute lowering of brain tyrosine is well tolerated, but only modestly lowers brain DA levels. Modification of tyrosine depletion to robustly lower DA levels would provi...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-013-2977-0
更新日期:2013-05-01 00:00:00
abstract::Monoamine oxidase inhibitors (MAOIs) are widely accepted as effective antidepressants. Unfortunately their use has been limited by their capacity to potentiate dietary tyramine (the so called "cheese effect") and their interaction with other drugs. The latter poses a particular problem for patients undergoing anaesthe...
journal_title:Psychopharmacology
pub_type: 杂志文章,评审
doi:10.1007/BF02246234
更新日期:1992-01-01 00:00:00
abstract::Sixteen healthy volunteers were administered midazolam followed by placebo or the benzodiazepine antagonist, flumazenil, in a double-blind, cross-over study. Flumazenil reversed midazolam-induced sedation on the subjective, psychophysiological and motor indices used. In contrast, there was little evidence of any rever...
journal_title:Psychopharmacology
pub_type: 临床试验,杂志文章
doi:10.1007/BF02244252
更新日期:1991-01-01 00:00:00
abstract::Adult hooded rats exposed to a repeated maternal separation procedure during the neonatal period showed a blunted expression of locomotor hyperactivity conditioned to the presentation of the daily food ration. We have demonstrated that the expression of food-conditioned anticipatory hyperactivity is sensitive to the r...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF02246414
更新日期:1996-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Footshock stress reliably reinstates heroin seeking in rats, but the time course of the development of this effect following drug withdrawal is not known. Here we studied the effect of intermittent footshock stress on reinstatement of heroin seeking following different withdrawal periods (1-66 ...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s002130100748
更新日期:2001-06-01 00:00:00
abstract:RATIONALE:Endogenous γ-aminobutyric acidA receptor (GABAAR)-active neurosteroids (e.g., allopregnanolone) regulate central nervous system excitability and many physiological functions, so fluctuations are implicated in several neuropsychiatric disorders. Pertinently, evidence supports an inverse relationship between en...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-017-4671-0
更新日期:2017-09-01 00:00:00
abstract::When tested in adulthood, male rats that had been treated daily with 50 mg/kg hydroxyzine HCl SC at 10--29 or 23--29 days of age were significantly facilitated in performance of delayed spontaneous alternation relative to saline-injected rats. Treatment at 10--16 days of age did not produce significant facilitation in...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00427646
更新日期:1980-01-01 00:00:00
abstract::The effects of d-fenfluramine and morphine on various nociceptive responses of rats were investigated. Unlike morphine, which inhibited all the responses examined, d-fenfluramine inhibited jumping and paw licking of rats on a hot plate, but did not increase the latency of tail withdrawal from hot water. The effects of...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00435276
更新日期:1982-01-01 00:00:00
abstract::Two methods were used to assess nicotine-induced antinociception: tail withdrawal from a hot water bath and hind paw withdrawal from a hotplate. Nicotine doses which produced 75-80% maximum response were 0.75 mg/kg (free base) for tail withdrawal and 0.35 mg/kg for paw withdrawal. The peripheral blocker chlorisondamin...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF02246552
更新日期:1995-12-01 00:00:00
abstract::It has been suggested that anxiety may be a critical factor in certain forms of non-opioid environmental analgesia. In the present study, 5-min exposure to the elevated plus-maze test of anxiety (EPM) induced a mild, though enduring, elevation in tail-flick latencies in male mice. Pretreatment with the opiate antagoni...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF02247133
更新日期:1990-01-01 00:00:00
abstract:RATIONALE:We have shown that differences in the level of neural activation to stimuli associated with smoking vs. natural rewards, a biomarker related to reward sensitivity, predict treatment outcome. OBJECTIVES:This paper examined whether this biomarker moderates the impact of bupropion or varenicline on smoking cess...
journal_title:Psychopharmacology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00213-017-4580-2
更新日期:2017-06-01 00:00:00
abstract:RATIONALE:Recent studies suggest that orexin/hypocretin is involved in drug reward and drug-seeking behaviors, including ethanol self-administration. However, orexin's role in ethanol-induced seeking behaviors remains unclear. OBJECTIVE:These studies examined the role of orexin in the acquisition and expression of eth...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-010-2082-6
更新日期:2011-04-01 00:00:00
abstract::Rats were treated once with doses of haloperidol or of droperidol below and above the acute ID50 vs the dopamine agonist apomorphine; they were later challenged with an acute dose of apomorphine (0.3 mg/kg, SC) and rated for stereotyped behavioral responses. The two neuroleptics were similar in acute anti-apomorphine ...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00431801
更新日期:1985-01-01 00:00:00
abstract::The novel nicotinic agonist 1-acetyl-4-methylpiperazine (AMP) has been studied in ligand-binding and behavioural studies. AMP methiodide potently inhibited [3H]-(-)-nicotine and [125I]-alpha-bungarotoxin binding to P2 membranes from rat brain and [125I]-alpha-bungarotoxin binding to rat skeletal muscles. AMP HCl also ...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF02251292
更新日期:1993-01-01 00:00:00
abstract::Aggressive rats could be differentiated from non-aggressive rats on a lower rate of rearing reactions in the open-field activity, as well as on a weaker aptitude for learning, increasing with the complication of the test. The two groups of rats had comparable activity, and a comparable response to pain. Apomorphine de...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00423303
更新日期:1976-07-09 00:00:00
abstract::The behavioral effects of the benzodiazepine (BZP)-receptor partial inverse agonists, beta-CCE and FG 7142, are reviewed and the claim that these compounds possess "anxiogenic" properties is examined. Results obtained from human studies and global observations in animals, as well as those from experiments on aggressio...
journal_title:Psychopharmacology
pub_type: 杂志文章,评审
doi:10.1007/BF00212837
更新日期:1988-01-01 00:00:00
abstract:RATIONALE:Repeated exposure to psychostimulant drugs causes a long-lasting increase in the psychomotor and reinforcing effects of these drugs and an array of neuroadaptations. One such alteration is a hypersensitivity of striatal activity such that a low dose of amphetamine in sensitized animals produces dorsal striata...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-008-1100-4
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To compare matched paliperidone-ER- and olanzapine-treated schizophrenic patients on measures of glucose and lipid metabolism. METHODS:Eighty hospitalized patients with schizophrenia (DSM-IV) were randomly assigned to treatment with paliperidone ER or olanzapine for a period of 12 weeks. At baseline and ever...
journal_title:Psychopharmacology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00213-013-3073-1
更新日期:2013-11-01 00:00:00
abstract:RATIONALE:Cue-elicited craving and stress responses have been identified as predictors of relapse in drug dependence, but little research exists on the contribution of these factors to marijuana use specifically. OBJECTIVES:The aims of the present study were to evaluate (1) responses to a psychological stressor, (2) r...
journal_title:Psychopharmacology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00213-011-2376-3
更新日期:2011-11-01 00:00:00
abstract::In a series of experiments, rats received the noncompetitive N-methyl-D-aspartate (NMDA) antagonist MK-801 and measures were made of motor behavior, spatial navigation in a swimming pool, and electroencephalographic (EEG) activity. High doses (0.25-10 mg/kg IV) produced somnolence and akinesia, impaired food consumpti...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF00441949
更新日期:1989-01-01 00:00:00
abstract:RATIONALE:The motivational impetuses underlying self-administration of cocaine and other drugs of abuse are not fully understood. One emerging factor is affect. Both positive and negative affective states have been hypothesized to influence drug seeking and drug taking. In parallel, it has been posited that the ultraso...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-010-1913-9
更新日期:2010-09-01 00:00:00
abstract:INTRODUCTION:Smoking rates among depressed individuals is higher than among healthy subjects, and nicotine alleviates depressive symptoms. Nicotine increases serotonergic and noradrenergic neuronal activity and facilitates serotonin and noradrenaline release. In mice, acute nicotine administration enhances the activity...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-009-1560-1
更新日期:2009-08-01 00:00:00
abstract::We have studied striatal D2 dopamine binding in schizophrenic patients treated with the novel atypical antipsychotic drug, olanzapine. 123I iodobenzamide (IBZM) single photon emission tomography (SPET) was used to estimate striatal dopamine D2 receptor binding in vivo. Patients were recruited from a prospective, doubl...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/BF02245615
更新日期:1996-03-01 00:00:00
abstract:RATIONALE:Deficits in response inhibition associated with heroin use could last several months after abstinence in heroin users, and their response inhibition can also be interfered with task-irrelevant drug-related cues. However, it is unclear whether exposure to drug-related cues affects subsequent response inhibitio...
journal_title:Psychopharmacology
pub_type: 杂志文章
doi:10.1007/s00213-019-05434-6
更新日期:2020-04-01 00:00:00