Abstract:
BACKGROUND:The focal goal of this study is to identify optimal accumulation periods for ALA-induced PpIX in different healthy anatomical sites of human oral cavity and different types of abnormal mucosa to improve the accuracy of the clinical applications such as photodiagnosis and tissue grading. MATERIALS AND METHODS:Laser-induced fluorescence (LIF) emission spectra, with excitation at 404 nm from a diode laser, were recorded with a miniature fiber-optics spectrometer from 13 anatomical sites of oral mucosa in 15 healthy volunteers and 30 suspicious sites in 15 patients after topical application of 0.4% 5-ALA solution for 15 min. The optimal accumulation time in different anatomical sites of healthy subjects and abnormal tissues were determined by studying the temporal variation in normalized fluorescence intensities (NFI) at 635, 685 and 705 nm. RESULTS AND DISCUSSIONS:In masticatory anatomical locations such as (gingival and hard palate) and in lining mucosa (inner lip, soft palate, floor of mouth, transition to floor of mouth, alveolus and ventral tongue) except vermillion border of lip (VBL) of healthy subjects (designated as group I), it was observed that optimum time for maximum accumulation of PpIX is 90 min. In comparison, for lateral side of tongue (LST) and dorsal side of tongue (DST) tissues (designated as group II), maximum accumulation of PpIX was observed in 150 min of ALA application. For diverse grade lesions of group I mucosa in patients, maximum accumulation of PpIX was observed in 90 min, whereas, in group II mucosa the optimum accumulation time was 150 min as in the case of healthy mucosa. Further, between different grades oral mucosa, maximum variation in NFI take place at these optimal time periods. CONCLUSIONS:The determination of the optimum accumulation time of ALA in oral mucosa based on NFI helps to improve the diagnostic contrast and accuracy of oral cancer diagnosis, and to plan appropriate timing for ensuing PDT.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Mallia RJ,Subhash N,Sebastian P,Kumar R,Thomas SS,Mathews A,Madhavan Jdoi
10.1016/j.pdpdt.2010.06.006subject
Has Abstractpub_date
2010-09-01 00:00:00pages
162-75issue
3eissn
1572-1000issn
1873-1597pii
S1572-1000(10)00071-2journal_volume
7pub_type
杂志文章abstract:BACKGROUND:Early diagnosis of non-melanoma skin cancer (NMSC) is potentially possible using optical coherence tomography (OCT) which provides non-invasive, real-time images of skin with micrometre resolution and an imaging depth of up to 2mm. OCT technology for skin imaging has undergone significant developments, impro...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.08.004
更新日期:2016-12-01 00:00:00
abstract::One of the factors limiting photodynamic therapy (PDT) is hypoxia in tumor cells during photodynamic action. PDT with pulse mode irradiation and appropriate irradiation parameters could be more effective in the singlet oxygen generation and tissue re-oxygenation than continuous wave (CW) mode. We theoretically demonst...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.01.003
更新日期:2016-03-01 00:00:00
abstract::Head and neck (H&N) cancer patients have a high incidence of local field change as well as second primary lung tumours. We have applied the Wolf Diagnostic Autofluorescence Endoscopy (DAFE) system in a novel way, combining autofluorescence evaluation of both H&N region and bronchial. New H&N cases as well as "old" cas...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2006.07.004
更新日期:2006-12-01 00:00:00
abstract::Decreasing incubation time, as well as enhanced PpIX production, are present challenges for topical photodynamic therapy (PDT). There are reports concerning the existence of a strong relationship between site temperature and porphyrin synthesis in biological tissue, that suggest temperature increase in the tissue can ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101786
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Optical coherence tomography (OCT) is a real-time non-invasive imaging tool, introduced in dermatology in the late 1990s. OCT uses near-infrared light impulses to produce images which can be displayed in cross-sectional and en-face mode. The technique has been used to image skin diseases especially non-melan...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2017.02.003
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer phototherapy that utilizes monoclonal antibody-IRDye700DX conjugates (mAb-IR700) and NIR light. We previously reported that mAb-IR700 aggregated on the plasma membrane and induced physical damage within the membrane, leading to necrotic/i...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101926
更新日期:2020-09-01 00:00:00
abstract::This study reports successful photodynamic inactivation of planktonic and biofilm cells of Enterococcus faecalis using Methylene Blue (MB) in combination with gold nanoparticles synthesized using the cell-free filtrate obtained from 3-day biomass of Trichoderma asperellum strain. Monodispersed colloidal gold nanoparti...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.05.042
更新日期:2019-09-01 00:00:00
abstract::Photodynamic therapy (PDT) is a common treatment on retinal capillary hemangioma. We applied PDT to a patient with von Hippel-Lindau(VHL) syndrome and she developed severe massive exudative retinal detachment the next day, which is a rare complication for PDT. After intraocular anti-VEGF agent and peribulbar dexametha...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.02.013
更新日期:2014-06-01 00:00:00
abstract::Medication-related osteonecrosis of the jaw (MRONJ) results in progressive destruction of the jawbones, and advanced cases demand surgical intervention. The total removal of necrotic bone is required to prevent recurrence and infection. However, determining the limits between necrotic and healthy bone is a challenge. ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2020.102003
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:The potential application of TOOKAD(®)-PDT for the treatment of blood vessels was investigated. TOOKAD(®) (WST09), a novel palladium-bacteriopheophorbide absorbs light in the near IR with a high quantum yield of intersystem crossing. Our study assessed the efficacy of this drug in inducing vascular damage wi...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(05)00006-2
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Condyloma acuminatum is a highly infectious disease caused by the human papillomavirus. Previous studies have shown that 5-aminolevulinic acid photodynamic therapy can inhibit proliferation of condyloma acuminatum keratinocytes. However, the effect of 5-aminolevulinic acid photodynamic therapy on condyloma a...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.018
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Light fractionation with a 2-h dark interval increases the efficacy of topical aminolevulinic acid (ALA) photodynamic therapy (PDT). Hexyl-aminolevulinate (HAL) is the hexyl ester of ALA. Both HAL and ALA lead to protoporphyrin IX (PpIX) accumulation in endothelial cells and to vascular effects, which are im...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.09.002
更新日期:2013-12-01 00:00:00
abstract::Glucose is the main energy substance to drive the physiological events of the cell.. Malignant cells exhibit a much higher rate of glycolysis than healthy cells to relieve the increased needs of energy. The higher metabolic rate induces the over-expression of the Glucose Transporter (GLUT) to transport more glucose in...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.102014
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-f...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.08.013
更新日期:2016-12-01 00:00:00
abstract::Non-melanotic cutaneous cancers and melanoma are the main common type skin cancers worldwide. Despite many therapeutic options, therapeutic efficacy is not satisfied in all patients with advanced skin cancers, especially in melanoma. Photodynamic therapy (PDT) is a technology for skin disease treatment because of its ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.08.023
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Optical fibres can be placed in balloons to deliver light for intracranial photodynamic therapies. METHOD:A series of fibres, with either cleaved ends or terminating in cylindrical diffusers, housed in two different balloons, containing various solutions were investigated. The aim of the experiments perform...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2007.06.003
更新日期:2007-09-01 00:00:00
abstract:BACKGROUND:Tumor markers are commonly used for cancer screening and as indicators of therapeutic effects. Certain types of tumor have been known to produce a variety of porphyrins after 5-aminolevulinic acid (ALA) administration. In this study, porphyrins in tumor-bearing mouse urine were analyzed after oral administra...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.04.004
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy (PDT) is a highly efficient approach for tumour therapy, though it also has its drawbacks, too. There are multiple mechanisms involved in cell death regulation that can be successfully targeted for improvement of PDT in particular cases. We assumed, however, that the potential to manage ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.04.003
更新日期:2013-12-01 00:00:00
abstract::Photodynamic effect was a chance discovery early in the 1900s, demonstrating the lethal effects of light activated chemicals on living cells. Although the application of the principles of photodynamic effect to patients' treatment and what became photodynamic therapy (PDT) was practiced in 1960s and 1970s, clinical tr...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2008.04.004
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to develop a novel photosensitizer from traditional plant extracts and to investigate the photodynamic therapy (PDT) effect and mechanism of action of the novel photosensitizer on KB and Hep-2 cells. METHODS:Fluorescence emission, cell viability, and intracellular distribution of c...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.02.020
更新日期:2019-06-01 00:00:00
abstract::Hidradenitis Suppurativa (HS) is a distressing painful chronic inflammatory skin follicular condition. It is a challenge to achieve better therapeutic effect and lower recurrence rate. In this report, ALA-PDT combined with surgery for the treatment achieved ulcer healing, pain elimination, with no relapse during our f...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.024
更新日期:2019-12-01 00:00:00
abstract::Hypericin photodynamic therapy (HypPDT) has been found to be an efficient inducer of cell death. However, there are indications that HypPDT also activates rescuing pathways. Cell responses to HypPDT are highly dependent on the Hyp intracellular localization and accumulation. We have shown previously that in U87 MG cel...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.02.010
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:Recently, photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) has gained attention for the diagnosis of neoplastic diseases. In the present study, anin vitro method of photodynamic cytodiagnosis (PDCD) performed using the reagent 5-ALA in the cytodiagnosis of solid pancreatic tumors was developed. Her...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.101581
更新日期:2019-10-16 00:00:00
abstract::Four tetracationic porphyrins have been investigated for their ability to photo-inactivate Leishmania major promastigotes. Parallel photocytotoxicity assays against keratinocytes and macrophages show significant differences in activity between the microorganism and mammalian cells. Results suggest that it may be possi...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2006.04.004
更新日期:2006-09-01 00:00:00
abstract::Photodynamic therapy (PDT) was started in the People's Republic of China in the early 1980s after domestically produced hematoporphyrin derivative (HpD) became available. Since then, numerous photosensitizers have been synthesized and evaluated. Several promising ones have undergone clinical investigations and a few h...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(06)00009-3
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Autofluorescence bronchoscopy (AFB) is a highly sensitive tool for the detection of early bronchial cancers. However, its specificity remains limited due to primarily false positive results induced by hyperplasia, metaplasia and inflammation. We have investigated the potential of blue-violet backscattered li...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2008.01.005
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:Verrucous carcinoma of the penis is a rare disease characterized by verrucous lesions. The traditional treatment methods for verrucous carcinoma result in loss of sexual function are not satisfactory. The purpose of this study was to evaluate the safety and effect of topical aminolevulinic acid photodynamic ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.08.015
更新日期:2018-12-01 00:00:00
abstract::Hepatitis is one of the most common life threatening diseases. The diagnosis is mainly based on biochemical analysis such as liver function test. However, histopathological evaluation of liver serves far better for more accurate final diagnosis. The goal of our study was to evaluate the eosin fluorescence pattern in C...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2017.03.016
更新日期:2017-09-01 00:00:00
abstract::We report the case of a patient with recurrence of follicular carcinoma of the thyroid 8 years after surgical resection followed by external beam radiotherapy and radio-iodine treatment. The patient was treated by endoscopic photodynamic therapy (PDT) with complete endoscopic response after 12 months with good symptom...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(04)00016-X
更新日期:2004-05-01 00:00:00
abstract::Antimicrobial photodynamic therapy uses a nontoxic photosensitizer with the assistance of harmless visible light to activate the photosensitizer. Consequently, the excited state of the photosensitizer interacts with molecular oxygen to produce reactive oxygen species, which have the antimicrobial effect. In this study...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.09.018
更新日期:2018-12-01 00:00:00