Abstract:
:Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kong M,Cutts EE,Pan D,Beuron F,Kaliyappan T,Xue C,Morris EP,Musacchio A,Vannini A,Greene ECdoi
10.1016/j.molcel.2020.04.026subject
Has Abstractpub_date
2020-07-02 00:00:00pages
99-114.e9issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(20)30268-9journal_volume
79pub_type
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