Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.

Abstract:

:Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Kong M,Cutts EE,Pan D,Beuron F,Kaliyappan T,Xue C,Morris EP,Musacchio A,Vannini A,Greene EC

doi

10.1016/j.molcel.2020.04.026

subject

Has Abstract

pub_date

2020-07-02 00:00:00

pages

99-114.e9

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(20)30268-9

journal_volume

79

pub_type

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