p53 and p21(Waf1) are recruited to distinct PML-containing nuclear foci in irradiated and Nutlin-3a-treated U2OS cells.

Abstract:

:Promyelocytic leukemia nuclear bodies (PML-NBs) are multiprotein complexes that include PML protein and localize in nuclear foci. PML-NBs are implicated in multiple stress responses, including apoptosis, DNA repair, and p53-dependent growth inhibition. ALT-associated PML bodies (APBs) are specialized PML-NBs that include telomere-repeat binding-factor TRF1 and are exclusively in telomerase-negative tumors where telomere length is maintained through alternative (ALT) recombination mechanisms. We compared cell-cycle and p53 responses in ALT-positive cancer cells (U2OS) exposed to ionizing radiation (IR) or the p53 stabilizer Nutlin-3a. Both IR and Nutlin-3a caused growth arrest and comparable induction of p53. However, p21, whose gene p53 activates, displayed biphasic induction following IR and monophasic induction following Nutlin-3a. p53 was recruited to PML-NBs 3-4 days after IR, approximately coincident with the secondary p21 increase. These p53/PML-NBs marked sites of apparently unrepaired DNA double-strand breaks (DSBs), identified by colocalization with phosphorylated histone H2AX. Both Nutlin-3a and IR caused a large increase in APBs that was dependent on p53 and p21 expression. Moreover, p21, and to a lesser extent p53, was recruited to APBs in a fraction of Nutlin-3a-treated cells. These data indicate (1) p53 is recruited to PML-NBs after IR that likely mark unrepaired DSBs, suggesting p53 may either be further activated at these sites and/or function in their repair; (2) p53-p21 pathway activation increases the percentage of APB-positive cells, (3) p21 and p53 are recruited to ALT-associated PML-NBs after Nutlin-3a treatment, suggesting that they may play a previously unrecognized role in telomere maintenance.

journal_name

J Cell Biochem

authors

Shen H,Maki CG

doi

10.1002/jcb.22852

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1280-90

issue

5

eissn

0730-2312

issn

1097-4644

journal_volume

111

pub_type

杂志文章
  • Light and electron microscopic immunocytochemical localization of two major proteins in garlic bulb.

    abstract::Garlic is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, antidiabetic, and anticoagulant. Two major proteins of 40 KD and 14 KD constituting approximately 96% of total garlic proteins have been recently purified at our Institute. This immunocytochemic...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240580411

    authors: Wen GY,Mato A,Wisniewski HM,Malik MN,Jenkins EC,Sheikh AM,Kim KS

    更新日期:1995-08-01 00:00:00

  • Are primary cultures realistic models of prostate cancer?

    abstract::Primary cultures fill a unique niche among the repertoire of in vitro model systems available to investigate the biology of the normal and malignant human prostate. This review summarizes some of the properties of primary cultures, with special emphasis on two questions: are primary cultures from adenocarcinomas reall...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10691

    authors: Peehl DM

    更新日期:2004-01-01 00:00:00

  • Novel leptin receptor signaling mutants identify location and sex-dependent modulation of bone density, adiposity, and growth.

    abstract::Leptin, a hormone primarily produced by adipocytes, contributes to the regulation of bone health by modulating bone density, growth and adiposity. Upon leptin binding, multiple sites of the long form of the leptin receptor (LepRb) are phosphorylated to trigger activation of downstream signaling pathways. To address th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27726

    authors: McCabe IC,Fedorko A,Myers MG Jr,Leinninger G,Scheller E,McCabe LR

    更新日期:2019-03-01 00:00:00

  • Stabilization of cellular properties and differentiation mutilpotential of human mesenchymal stem cells transduced with hTERT gene in a long-term culture.

    abstract::Human bone marrow mesenchymal stem cells (hMSCs) are promising candidates for cell therapy and tissue engineering. The life span of hMSCs during in vitro culture is limited. Human telomerase catalytic subunit (hTERT) gene transduction can prolong the life span of hMSCs and maintain their potential of osteogenic differ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21502

    authors: Huang G,Zheng Q,Sun J,Guo C,Yang J,Chen R,Xu Y,Wang G,Shen D,Pan Z,Jin J,Wang J

    更新日期:2008-03-01 00:00:00

  • cdk5 modulates beta- and delta-catenin/Pin1 interactions in neuronal cells.

    abstract::The cdk5/p35 complex has been implicated in a variety of functions related to brain development, including axonal outgrown and neuronal migration. In this study, by co-immunoprecipitation and pull-down experiments, we have shown that the cdk5/p35 complex associates with and phosphorylates the neuronal delta-catenin. I...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21041

    authors: Muñoz JP,Huichalaf CH,Orellana D,Maccioni RB

    更新日期:2007-02-15 00:00:00

  • Identification of functional circRNA/miRNA/mRNA regulatory network for exploring prospective therapy strategy of colorectal cancer.

    abstract::Circular RNA (circRNA) has been reported to have great scientific significance and clinical value in multiple cancers including colorectal cancer (CRC). However, the biological function of most circRNAs in CRC is still in its infancy. Herein, we discovered the differential expressed circRNAs (DECs) between CRC tissues...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29703

    authors: Xu D,Wu Y,Wang X,Hu X,Qin W,Li Y,Wang Y,Zhang Z,Lu S,Sun T,Wu Z,Fu D,Fu B,Zhang J,Chen Q,Wei M,Zhao L,Wu H

    更新日期:2020-03-01 00:00:00

  • Membrane dynamics of γ-secretase with the anterior pharynx-defective 1B subunit.

    abstract::The four-subunit protease complex γ-secretase cleaves many single-pass transmembrane (TM) substrates, including Notch and β-amyloid precursor protein to generate amyloid-β (Aβ), central to Alzheimer's disease. Two of the subunits anterior pharynx-defective 1 (APH-1) and presenilin (PS) exist in two homologous forms AP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29832

    authors: Dehury B,Kepp KP

    更新日期:2021-01-01 00:00:00

  • The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.

    abstract::The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or suspended in collagen I to form spot cultures (SCs). Our laboratory has sh...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23025

    authors: Nugent AE,McBurney DL,Horton WE Jr

    更新日期:2011-04-01 00:00:00

  • Characterization of TBX20 in human hearts and its regulation by TFAP2.

    abstract::The T-box family of transcription factors has been shown to have major impact on human development and disease. In animal studies Tbx20 is essential for the development of the atrioventricular channel, the outflow tract and valves, suggesting its potential causative role for the development of Tetralogy of Fallot (TOF...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21686

    authors: Hammer S,Toenjes M,Lange M,Fischer JJ,Dunkel I,Mebus S,Grimm CH,Hetzer R,Berger F,Sperling S

    更新日期:2008-06-01 00:00:00

  • Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.

    abstract::We report the amino acid sequence, genomic organization, tissue expression, and alternative splice patterns for the human kinase related protein (KRP) gene, as well as the discovery of a new CA repeat sequence polymorphic marker in an upstream intron of the myosin light chain kinase (MLCK) gene. The KRP/MLCK genetic l...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Watterson DM,Schavocky JP,Guo L,Weiss C,Chlenski A,Shirinsky VP,Van Eldik LJ,Haiech J

    更新日期:1999-12-01 00:00:00

  • Interference of bovine herpesvirus 1 (BHV-1) in sorbitol-Induced apoptosis.

    abstract::In order to determine the ability of bovine herpesvirus type 1 (BHV-1) to suppress apoptosis, we examined the effects of BHV-1 infection on sorbitol-induced apoptosis on Madin-Darby bovine kidney (MDBK) cells. BHV-1 suppresses sorbitol-induced apoptosis in a manner similar to that of herpes simplex virus type 1 (HSV-1...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10518

    authors: De Martino L,Marfé G,Di Stefano C,Pagnini U,Florio S,Crispino L,Iovane G,Macaluso M,Giordano A

    更新日期:2003-05-15 00:00:00

  • CaM kinase control of AKT and LNCaP cell survival.

    abstract::AKT and its substrate BAD have been shown to promote prostate cancer cell survival. Agonists, such as carbachol, and hormones that increase intracellular calcium concentration can activate AKT leading to cancer cell survival. The LNCaP prostate cancer cells express the carbachol-sensitive M(3) -subtype of G protein-co...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24020

    authors: Schmitt JM,Smith S,Hart B,Fletcher L

    更新日期:2012-05-01 00:00:00

  • Albumin induces cellular fibrosis by upregulating transforming growth factor-beta ligand and its receptors in renal distal tubule cells.

    abstract::Albuminuria is indicative of nephropathy. However, little literature has focused on the role of albumin in renal distal tubule fibrosis. We used a well-defined distal tubule cell, Madin-Darby Canine Kidney (MDCK). Proliferation and cytotoxicity were examined. The conditioned supernatant was collected and subjected to ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20673

    authors: Liu HC,Liao TN,Lee TC,Chuang LY,Guh JY,Liu SF,Hu MS,Yang YL,Lin SH,Hung MY,Huang JS,Hung TJ,Chen CD,Chiang TA,Chan JY,Chen SY,Yang YL

    更新日期:2006-04-01 00:00:00

  • The control of fracture healing and its therapeutic targeting: improving upon nature.

    abstract::Fracture repair is a complex process involving timed cellular recruitment, gene expression, and synthesis of compounds that regenerate native tissue to restore the mechanical integrity, and thus function of injured bone. While the majority of fractures heal without complication, this takes time and a subset of patient...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22418

    authors: Komatsu DE,Warden SJ

    更新日期:2010-02-01 00:00:00

  • Inhibitory effect of paeoniflorin on the inflammatory vicious cycle between adipocytes and macrophages.

    abstract::Obesity is associated with a state of chronic, low-grade inflammation. It is considered that the paracrine loop involving free fatty acid (FFA) and tumor necrosis factor (TNF)α between adipocytes and macrophages establishes an inflammatory vicious cycle that augments the inflammatory changes and insulin resistance in ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22173

    authors: Jiang B,Qiao J,Yang Y,Lu Y

    更新日期:2012-08-01 00:00:00

  • Expression of bone-specific genes by hypertrophic chondrocytes: implication of the complex functions of the hypertrophic chondrocyte during endochondral bone development.

    abstract::Endochondral bone formation is one of the most extensively examined developmental sequences within vertebrates. This process involves the coordinated temporal/spatial differentiation of three separate tissues (cartilage, bone, and the vasculature) into a variety of complex structures. The differentiation of chondrocyt...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-4644(199607)62:1%3C1::AID-JCB1%

    authors: Gerstenfeld LC,Shapiro FD

    更新日期:1996-07-01 00:00:00

  • ERK1 Directly Interacts With JNK1 Leading to Regulation of JNK1/c-Jun Activity and Cell Transformation.

    abstract::ERK1 and ERK2 share a great deal of homology and have been presumed to have similar functions. Available antibodies recognize both isoforms making the elucidation of functional differences challenging. Mitogen-activated protein (MAP) kinase networks are commonly depicted in the literature as linear and sequential phos...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25896

    authors: Kang BS,Hwang YJ,Dong Z

    更新日期:2017-08-01 00:00:00

  • Plasma inter-alpha-trypsin inhibitor-related urinary glycoprotein EDC1 inhibits the growth of a Burkitt's lymphoma cell line.

    abstract::A homogeneous preparation of a urinary glycoprotein has been isolated from urine of patients with malignant melanoma and advanced adenocarcinomas of colon and lung. This molecule, Mr 30 kDa, is homologous to EDC1, a proteinase inhibitor antigenically related to plasma inter-alpha-trypsin inhibitor (IATI) originally is...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240420404

    authors: Chawla RK,Lawson DH,Travis J

    更新日期:1990-04-01 00:00:00

  • The function of BED finger domain of Zbed3 in regulating lung cancer cell proliferation.

    abstract::Zbed3, a BED finger domain-containing protein was found to promote cancer proliferation by regulating β-catenin expression through interacting with Axin. But whether and how BED finger domain function in regulating cancer proliferation is unknown. We constructed five mutants of Zbed3, which lacks the Axin-Zbed3 bindin...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28498

    authors: Liu H,Shi X,Fan X,Zhang D,Jiang B,Zhao Y,Fan C

    更新日期:2019-08-01 00:00:00

  • Initiation of proliferative events by human alpha-thrombin requires both receptor binding and enzymic activity.

    abstract::To determine the role of thrombin high-affinity receptor occupancy and enzymic activity in thrombin initiation of cell proliferation, we have utilized thrombin derivatives which separate these functions. We previously showed that enzymically active gamma-thrombin stimulates ion fluxes without binding to high-affinity ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240260306

    authors: Carney DH,Stiernberg J,Fenton JW 2nd

    更新日期:1984-01-01 00:00:00

  • Modulation of uptake of organic cationic drugs in cultured human colon adenocarcinoma Caco-2 cells by an ecto-alkaline phosphatase activity.

    abstract::Alkaline phosphatase (ALP) refers to a group of nonspecific phosphomonoesterases located primarily in cell plasma membrane. It has been described in different cell lines that ecto-ALP is directly or indirectly involved in the modulation of organic cation transport. We aimed to investigate, in Caco-2 cells, a putative ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10306

    authors: Calhau C,Martel F,Hipólito-Reis C,Azevedo I

    更新日期:2002-01-01 00:00:00

  • Differential display of human marrow stromal cells reveals unique mRNA expression patterns in response to dexamethasone.

    abstract::Human bone marrow stromal cells (hBMSC) are pluripotent cells that have the ability to differentiate into bone, cartilage, hematopoietic-supportive stroma, and adipocytes in a process modulated by dexamethasone (DEX). To characterize changes in hBMSC in response to DEX, we carried out differential display experiments ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(20000201)76:2<231::aid-jcb

    authors: Dieudonné SC,Kerr JM,Xu T,Sommer B,DeRubeis AR,Kuznetsov SA,Kim IS,Gehron Robey P,Young MF

    更新日期:1999-12-01 00:00:00

  • Furanodiene enhances tamoxifen-induced growth inhibitory activity of ERa-positive breast cancer cells in a PPARγ independent manner.

    abstract::Herbal plants are enriched with compounds with a wide range of biological activities. Furanodiene is a sesquiterpene isolated from Rhizoma Curcumae. Growing evidence shows furanodiene exhibits diversified activities of hepatoprotection, anti-inflammation, anti-angiogenesis, and anti-tumor. However, its biological acti...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24139

    authors: Zhong ZF,Li YB,Wang SP,Tan W,Chen XP,Chen MW,Wang YT

    更新日期:2012-08-01 00:00:00

  • Vanadium induces apoptosis and modulates the expressions of metallothionein, Ki-67 nuclear antigen, and p53 during 2-acetylaminofluorene-induced rat liver preneoplasia.

    abstract::Our previous studies have shown that vanadium, a dietary micronutrient, has an inhibitory effect against experimentally induced rat hepatocarcinogenesis. In this study, we evaluated the role of vanadium on some potential protein expression markers of carcinogenesis, such as metallothionein (MT), an intracellular metal...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20304

    authors: Chakraborty T,Samanta S,Ghosh B,Thirumoorthy N,Chatterjee M

    更新日期:2005-03-01 00:00:00

  • Identification of pathway-based recurrence-associated signatures in optimally debulked patients with serous ovarian cancer.

    abstract::Serous ovarian cancer (SOC) is the most common form of the histological subtype of epithelial ovarian cancer, with the worst clinical outcome. Despite improvements in surgery and chemotherapy, most patients with SOC experience recurrence within 12-18 months of first-line treatment. Current studies are unable to robust...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27098

    authors: Deng K,Zhang F,Song W,Zhao W,Rong Z,Cai Y,Xu H,Lu M,Wang W,Li A,Hou Y,Li Z,Li K

    更新日期:2018-11-01 00:00:00

  • Distinctive regulation and function of PI 3K/Akt and MAPKs in doxorubicin-induced apoptosis of human lung adenocarcinoma cells.

    abstract::Regulation and function of PI 3K/Akt and mitogen-activated protein kinases (MAPKs) in doxorubicin-induced cell death were investigated in human lung adenocarcinoma cells. Doxorubicin induced dose-dependent apoptosis of human lung adenocarcinoma NCI-H522 cells. Prior to cell death, both Akt and the MAPK family members ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10751

    authors: Zhao Y,You H,Yang Y,Wei L,Zhang X,Yao L,Fan D,Yu Q

    更新日期:2004-02-15 00:00:00

  • Prereplicative increase of nuclear matrix-bound DNA polymerase-alpha and primase activities in HeLa S3 cells following dilution of long-term cultures.

    abstract::We investigated the association of DNA polymerase and DNA primase activity with the nuclear matrix in HeLa S3 cells diluted with fresh medium after having been cultured without any medium change for 7 days. Flow cytometric analysis demonstrated that just before dilution about 85% of the cells were in the G1 phase of t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19981001)71:1<11::aid-jcb2

    authors: Martelli AM,Capitani S,Neri LM

    更新日期:1998-10-01 00:00:00

  • Antihepatotoxic benefits of Poria cocos polysaccharides on acetaminophen-lesioned livers in vivo and in vitro.

    abstract::In our previous study, preliminary data indicates that Poria cocos polysaccharides (PCP) shows beneficial hepatoprotection against acetaminophen (APAP)-induced liver injury in mice. However, biological molecular mechanism warrants to be further discussed. In current study, a number of biochemical tests and immunoassay...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28022

    authors: Wu K,Guo C,Yang B,Wu X,Wang W

    更新日期:2019-05-01 00:00:00

  • Pancreatic stellate cell-potentiated insulin secretion from Min6 cells is independent of interleukin 6-mediated pathway.

    abstract::Pancreatic stellate cells (PSCs) secrete various factors, which can influence the β-cell function. The identification of stellate cell infiltration into the islets in pancreatic diseases suggests possible existence of cross-talk between these cells. To elucidate the influence of PSCs on β-cell function, mouse PSCs wer...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29329

    authors: Bynigeri RR,Mitnala S,Talukdar R,Singh SS,Duvvuru NR

    更新日期:2020-01-01 00:00:00

  • Up-regulation of p21CIP1 expression mediated by ERK-dependent and -independent pathways contributes to hepatocyte growth factor-induced inhibition of HepG2 hepatoma cell proliferation.

    abstract::Strong activation of the ERK signal is required for hepatocyte growth factor (HGF) to inhibit proliferation of the human hepatocellular carcinoma cell line HepG2. However, it is still to be elucidated whether the activation alone is sufficient to induce the inhibitory effect. In this study, we constructed HepG2 cell c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21614

    authors: Shirako E,Hirayama N,Tsukada Y,Tanaka T,Kitamura N

    更新日期:2008-05-01 00:00:00