Identification of mouse mutant cells exhibiting the plastic mutant phenotype.

Abstract:

:The initial processes involved in radiation carcinogenesis have not been clearly elucidated. We isolated mouse mutant cells exhibiting plasticity in their mutation phenotypes. These mutant cells were originally isolated from an irradiated cell population as 6-thioguanine resistant (6TGR) mutants that were deficient in hypoxanthine phosphoribosyl transferase (Hprt, E.C.2.4.2.8) activity at the frequency of approximately 6.2 x 10(-5). Approximately 10% of 6TGR cells showed plasticity in their mutant phenotypes and reverted to HAT-resistant (HATR), which is Hprt-proficient, wild type phenotype. Eventually we identified the plastic mutants in the un-irradiated wild type cell population as well and found that ionizing irradiation enhanced the frequency of the plastic mutation approximately 24 times. Treatment with 5-aza-cytidine did not affect the plasticity of mutant phenotypes identified in this study, suggesting that DNA methylation was not involved in the plastic changes of the mutant phenotypes. The plastic mutant phenotype identified in our study is a new type of genomic instability induced by ionizing irradiation, and it is likely to be involved in one of the primary changes that occur in the process of radiation carcinogenesis, and may explain one element of carcinogenesis, which is composed of multi-stages.

journal_name

Biosci Trends

journal_title

Bioscience trends

authors

Yamauchi M,Fukutsu K,Sakagami M,Yamada Y

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

195-200

issue

4

eissn

1881-7815

issn

1881-7823

pii

333

journal_volume

4

pub_type

杂志文章
  • Protective effects of luteolin-7-O-glucoside against starvation-induced injury through upregulation of autophagy in H9c2 Cells.

    abstract::Cardiomyocyte nutrient deprivation is a common clinical event that mediates various cardiac ischemic processes and is associated with autophagy activation and cell survival or death. Luteolin-7-O-glucoside (LUTG) was one of the flavonoid glycosides isolated from Dracocephalum tanguticum. Previous research had showed t...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01111

    authors: Yao H,Zhou L,Tang L,Guan Y,Chen S,Zhang Y,Han X

    更新日期:2017-11-20 00:00:00

  • Pulse pressure variation and stroke volume variation predict fluid responsiveness in mechanically ventilated patients experiencing intra-abdominal hypertension.

    abstract::The purpose of the present study was to evaluate whether pulse pressure variation (PPV) and stroke volume variation (SVV) can predict fluid responsiveness in patients with intra-abdominal hypertension (IAH) in either a supine or Trendelenburg position. Forty mechanically ventilated patients that exhibited IAH resultin...

    journal_title:Bioscience trends

    pub_type: 临床试验,杂志文章

    doi:

    authors: Liu X,Fu Q,Mi W,Liu H,Zhang H,Wang P

    更新日期:2013-04-01 00:00:00

  • A case of advanced lung cancer with malignant pericardial effusion treated by intrapericardial Cinobufacini injection instillation.

    abstract::Malignant pericardial effusion is one of the severe complications in advanced lung cancer patients, seriously affecting the patient's cardiopulmonary function and even life. Pericardial drainage and instillation of anti-neoplastic drugs in the pericardial cavity seems to offer the best chance of controlling pericardia...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2014.01073

    authors: Sun T,Zhang Y,Shen Y,Hu K,Zuo M

    更新日期:2014-08-01 00:00:00

  • Are inflammation-based markers useful in patients with hepatocellular carcinoma and clinically significant portal hypertension after liver resection?

    abstract::Inflammation-based markers are considered prognostic indicators for patients with hepatocellular carcinoma (HCC) after liver resection. However, there is little information concerning whether they are useful for HCC patients with clinically significant portal hypertension (CSPH). In this study, 1452 patients were enro...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2020.03180

    authors: Qin L,Li C,Xie F,Wang Z,Wen T

    更新日期:2020-09-21 00:00:00

  • Detection of chromosome abnormalities using current noninvasive prenatal testing: A multi-center comparative study.

    abstract::Noninvasive prenatal testing (NIPT) is increasingly recognized and utilized in the antenatal care field. In the current study, we aimed to evaluate the clinical application and compare test outcomes of two generations of currently used NIPT techniques for detecting fetal chromosome abnormalities in a high-risk prenata...

    journal_title:Bioscience trends

    pub_type: 杂志文章,多中心研究

    doi:10.5582/bst.2018.01044

    authors: Du Y,Lin J,Lan L,Dong Y,Zhu J,Jiang W,Pan X,Lu Y,Li D,Wang L

    更新日期:2018-07-17 00:00:00

  • A Clinical scoring model to predict mortality in HIV/TB co-infected patients at end stage of AIDS in China: An observational cohort study.

    abstract::We construct and validate a non-invasive clinical scoring model to predict mortality in HIV/TB patients at end stage of AIDS in China. There were 1,007 HIV/TB patients admitted to Beijing Ditan Hospital from August 2009 to January 2018 included in this study, who were randomly assigned to form derivation cohort and va...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01309

    authors: Zhang Z,Xu L,Pang X,Zeng Y,Hao Y,Wang Y,Wu L,Gao G,Yang D,Zhao H,Xiao J

    更新日期:2019-05-12 00:00:00

  • Perspectives on human clinical trials of therapies using iPS cells in Japan: reaching the forefront of stem-cell therapies.

    abstract::A research project involving sheets of retinal pigment epithelium constructed from iPS cells derived from patients with age-related maculopathy is one step closer to being approved for clinical trials by the Japanese Government. Now is the time to make therapies using iPS cells clinically available. ...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Song P,Inagaki Y,Sugawara Y,Kokudo N

    更新日期:2013-06-01 00:00:00

  • Exploration of Salmonella effector mutant strains on MTR4 and RRP6 degradation.

    abstract::Salmonella enterica serovar Typhimurium (Salmonella), a pathogenic bacterium, is a major cause of foodborne diseases worldwide. Salmonella injects multiple virulence factors, called effectors, into cells and causes multiple rearrangements of cellular biological reactions that are important for Salmonella proliferation...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2020.03085

    authors: Sun X,Kawata K,Miki A,Wada Y,Nagahama M,Takaya A,Akimitsu N

    更新日期:2020-09-21 00:00:00

  • Bu-Shen-Ning-Xin decoction suppresses osteoclastogenesis via increasing dehydroepiandrosterone to prevent postmenopausal osteoporosis.

    abstract::Bu-Shen-Ning-Xin decoction (BSNXD), a traditional Chinese medicine, has been used to prevent and treat age-related diseases such as postmenopausal osteoporosis (PMO) for decades. This study sought to investigate the underlying mechanisms of BSNXD in terms of receptor activation of nuclear factor κB ligand (RANKL)-indu...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2015.01011

    authors: Gui Y,Qiu X,Xu Y,Li D,Wang L

    更新日期:2015-06-01 00:00:00

  • Performing laparoscopic surgery - Perspectives of young Chinese hepatobiliary surgeons.

    abstract::Laparoscopic liver resection (LLR) has garnered attention as a new form of liver surgery. In China, many hepatobiliary surgeons are now encouraging the examination and assessment of LLC in order to improve its outcomes, and several young hepatobiliary surgeons recently shared their clinical experiences and the results...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01086

    authors: Zhao X,Song P,Zhang Y,Huang J

    更新日期:2018-01-01 00:00:00

  • Sub-acute exposure to the herbicide atrazine suppresses cell immune functions in adolescent mice.

    abstract::Atrazine (ATR), one of the most widely used herbicides worldwide, has caused a series of toxicological and environmental problems. This study sought to investigate the effects of ATR on the immune system of mice. Four-week-old female C57B l/6 mice were treated with 5, 25, and 125 mg/kg ATR for 28 days. On day 29, bloo...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Zhao S,Liu J,Zhao F,Liu W,Li N,Suo Q,Zhao J,Zhao L

    更新日期:2013-08-01 00:00:00

  • Comparison between endoluminal ultrasonography and spiral computerized tomography for the preoperative local staging of rectal carcinoma.

    abstract::The aim of this study is to compare the efficacy of endoluminal ultrasonography (EUS) and spiral computerized tomography (SCT) in preoperative local staging of rectal carcinoma. EUS and SCT were performed prior to surgery in 78 patients with rectal carcinoma. After radical surgery, the preoperative findings were compa...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Ju H,Xu D,Li D,Chen G,Shao G

    更新日期:2009-04-01 00:00:00

  • Availability and use of emergency obstetric care services in public hospitals in Laos PDR: a systems analysis.

    abstract::The maternal mortality ratio in Laos in 2005 was 660 per 100,000 lives birth which was the third highest in Asia-Pacific Region. The objective was to determine the availability and use of emergency obstetric care (EmOC) in provincial and district hospitals in Borikhamxay, Khammouane, and Savannakhet provinces using UN...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Douangphachanh X,Ali M,Outavong P,Alongkon P,Sing M,Chushi K

    更新日期:2010-12-01 00:00:00

  • Bilirubin effect on endothelial adhesion molecules expression is mediated by the NF-kappaB signaling pathway.

    abstract::We have recently demonstrated that unconjugated bilirubin (UCB) limits the overexpression of adhesion molecules and inhibits the PMN endothelial adhesion induced by the pro-inflammatory cytokine TNFalpha. To understand the molecular events involved we investigated whether the inhibitory effect is determined by a direc...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Mazzone GL,Rigato I,Ostrow JD,Tiribelli C

    更新日期:2009-08-01 00:00:00

  • Relationships between neighborhood attributes and subjective well-being among the Chinese elderly: Data from Shanghai.

    abstract::It has been hypothesized that subjective well-being (SWB) is determined by a combination of individual characteristics, social environment, and physical environment. However, few studies have simultaneously examined the relationships of the social and physical attributes of a neighborhood with SWB. Accordingly, the pr...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01170

    authors: Gao J,Weaver SR,Fu H,Jia Y,Li J

    更新日期:2017-11-20 00:00:00

  • Comparison of the docetaxel concentration in human plasma measured with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and a nanoparticle immunoassay and clinical applications of that assay.

    abstract::To determine the feasibility of using a nanoparticle immunoassay for clinical therapeutic drug monitoring (TDM) of docetaxel concentrations, a sensitive and simple method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established to measure the docetaxel concentration in human plasma and the results ...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2017.01029

    authors: Geng C,Li P,Chen X,Yuan G,Guo N,Liu H,Zhang R,Guo R

    更新日期:2017-05-23 00:00:00

  • Rapid progression of encephalopathy in a patient with hepatitis B infection.

    abstract::The mortality rate of fulminant hepatic failure was high until liver transplantation was presented as a potential therapy. We encountered a patient with hyperacute fulminant hepatic failure due to hepatitis B virus infection. Living donor liver transplantation was planned but abandoned because her brain edema progress...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Takemura N,Sugawara Y,Tamura S,Kakeno J,Matsui Y,Makuuchi M

    更新日期:2007-08-01 00:00:00

  • Involvement of the central monoaminergic system in the antidepressant-like effect of catalpol in mice.

    abstract::Catalpol is a natural iridoid glycoside with diverse bioactivities that is found in abundance in Rehmannia glutinosa Libosch. (Scrophulariaceae). The present study assessed whether catalpol treatment (5, 10, or 20 mg/kg for 14 days by intragastric administration (i.g.)) has an antidepressant-like effect on mice perfor...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2014.01029

    authors: Wang J,Cui Y,Feng W,Zhang Y,Wang G,Wang X,Zhou G

    更新日期:2014-10-01 00:00:00

  • Long live the health care system in Japan.

    abstract::In Japan, a cost-containment policy has been implemented with health care system reform. Increasing medical expenses are inevitable due to aging of the population, health transitions, and expanded and advanced medical services. Policies such as the introduction of regressive reimbursement for inpatient services as par...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Idezuki Y

    更新日期:2008-04-01 00:00:00

  • The preliminary clinical observation and analysis of childbearingage women with a history of iodine-131 treatment for Graves' disease.

    abstract::Whether radioactive iodine treatment of Graves' disease (GD) during pregnancy will increase pregnancy loss and affect fetal development is still a matter of concern. From May 2005 to December 2015, 2,276 childbearing-age women with GD received iodine-131 treatment in our departments and were retrospectively enrolled i...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2016.01008

    authors: Guan L,Chen G,Zhang J,Wang L

    更新日期:2016-09-05 00:00:00

  • An international comparison analysis of reserve and supply system for emergency medical supplies between China, the United States, Australia, and Canada.

    abstract::Coronavirus disease 19 (COVID-19) has become a pandemic around the world. With the explosive growth of confirmed cases, emergency medical supplies are facing global shortage, which restricts the treatment of seriously ill patients and protection of medical staff. Taking China, the United States, Australia, and Canada ...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2020.03093

    authors: Wang X,Wu W,Song P,He J

    更新日期:2020-09-21 00:00:00

  • Substrate specificity of human granzyme 3: analyses of the P3-P2-P1 triplet using fluorescence resonance energy transfer substrate libraries.

    abstract::Granzyme 3 (Gr3) is known as a tryptase-type member of the granzyme family and exists in the granules of immunocompetent cells. Granule proteases including granzymes, are transported into the cytoplasm of tumor cells or virus-infected cells by perforin function, degrade cytoplasmic or nuclear proteins and subsequently...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.8.126

    authors: Hirata Y,Inagaki H,Shimizu T,Kawada T

    更新日期:2014-04-01 00:00:00

  • Medical students' choice of specialty and factors determining their choice: a cross-sectional questionnaire survey in Melaka-Manipal Medical College, Malaysia.

    abstract::Information about medical students' choice of specialty can be helpful for planning health manpower. However, such information from medical students in Malaysian medical schools is lacking. We carried out a cross-sectional questionnaire survey among fourth- and fifth-year medical undergraduate students at Melaka-Manip...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2011.v5.2.69

    authors: Chew YW,Rajakrishnan S,Low CA,Jayapalan PK,Sreeramareddy CT

    更新日期:2011-01-01 00:00:00

  • N-terminal PEGylation of human serum albumin and investigation of its pharmacokinetics and pulmonary microvascular retention.

    abstract::Human serum albumin (HSA) is used as an important plasma volume expander in clinical practice. In the present study, HSA was N-terminally PEGylated and a PEGylated HAS (PEG-HSA) carrying one chain of PEG (20 kDa) per HSA molecule was obtained. The purity, secondary structure and hydrodynamic radius of the modified pro...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Zhao T,Yang Y,Zong A,Tan H,Song X,Meng S,Song C,Pang G,Wang F

    更新日期:2012-04-01 00:00:00

  • Prevalence of HIV infection and HIV-related sex risk behaviors in men who have sex with men in Shandong Province, China.

    abstract::Recently, men who have sex with men (MSM) have become a population at high risk ofHIV infection in China, and more investigations of and intervention programs targeting this hidden population are urgently needed. The objective of this study was to assess the prevalence of HIV-positive individuals, HIV-related knowledg...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:

    authors: Tao X,Gai R,Zhang X,Zhang N,Zheng W,Xu A,Li S

    更新日期:2008-06-01 00:00:00

  • China issues the National Essential Medicines List (2018 edition): Background, differences from previous editions, and potential issues.

    abstract::On October 25, 2018, the National Health Commission of China issued the National Essential Medicines List (2018 edition) [NEML (2018)]. The NEML (2018) contains 685 drugs, which consist of 417 chemicals and biological products and 268 Chinese patent medicines. Compared to the 2012 version of the NEML, a total number o...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2018.01265

    authors: He J,Tang M,Ye Z,Jiang X,Chen D,Song P,Jin C

    更新日期:2018-01-01 00:00:00

  • Application of low-pressure cell seeding system in tissue engineering.

    abstract::Tissue engineering has been one of the most promising strategies for the regeneration of impaired tissue. Application of three-dimensional porous scaffolds has greatly improved the outcome of tissue engineering in many categories. Cell seeding is one of the key issues in tissue regeneration. It depends not only on the...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:

    authors: Dai W,Dong J,Chen G,Uemura T

    更新日期:2009-12-01 00:00:00

  • An overview of potential therapeutic agents to treat COVID-19.

    abstract::The emerging novel coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has swept across the world and become a global threat to public health. More than 200 countries and territories worldwide are suffering from this COVID-19 pandemic. Worryingly, no specific vaccine...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:10.5582/bst.2020.03345

    authors: Dong X,Tian Z,Shen C,Zhao C

    更新日期:2020-11-04 00:00:00

  • Adjuvant sorafenib reduced mortality and prolonged overall survival and post-recurrence survival in hepatocellular carcinoma patients after curative resection: a single-center experience.

    abstract::Adjuvant therapy after resection of hepatocellular carcinoma (HCC) is limited. Here, we evaluated the effects of postoperative sorafenib on recurrence and survival in HCC patients. Recurrence-free survival and overall survival were analyzed as the main endpoint, recurrence rate, and mortality rate were analyzed as sec...

    journal_title:Bioscience trends

    pub_type: 杂志文章

    doi:10.5582/bst.2014.01120

    authors: Zhang W,Zhao G,Wei K,Zhang Q,Ma W,Song T,Wu Q,Zhang T,Kong D,Li Q

    更新日期:2014-12-01 00:00:00

  • Traditional Chinese medicine for human papillomavirus (HPV) infections: A systematic review.

    abstract::Human papillomavirus (HPV) infections are common and generally harmless, but persistent infections can bring health problems like cancer and genital warts. For the uninfected group, HPV vaccines provide safe and effective protection, but they're type-restricted and expensive. For those infected, so far there have been...

    journal_title:Bioscience trends

    pub_type: 杂志文章,评审

    doi:10.5582/bst.2017.01056

    authors: Lin J,Chen L,Qiu X,Zhang N,Guo Q,Wang Y,Wang M,Gober HJ,Li D,Wang L

    更新日期:2017-07-24 00:00:00