Abstract:
OBJECTIVES:As Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the most commonly reported STIs in Belgium and the majority of women infected are asymptomatic, targeted screening of patients in specified risk groups is indicated. To prevent long-term complications and interrupt transmission, extragenital samples should be included. As this comes with a substantial extra cost, analysis of a pooled sample from vaginal and extragenital sites could be a solution. In this study, we evaluated the feasibility of molecular testing for CT and NG in pooled versus single-site samples in a large cohort of female sex workers. METHODS:Women were sampled from three anatomical sites: a pharyngeal, a vaginal and a rectal swab. Each sample was vortexed, and 400 µL of transport medium from each sample site was pooled into an empty tube. NAAT was performed using the Abbott RealTime CT/NG assay on the m2000sp/rt system. RESULTS:We included 489 patients: 5.1% were positive for CT; 2.0% were positive for NG and 1.4% were coinfected, resulting in an overall prevalence of 6.5% (95% CI 4.5% to 9.1%) for CT and 3.5% (95% CI 2.0% to 5.5%) for NG. From the 42 patients positive on at least one non-pooled sample, only 5 gave a negative result on the pooled sample, resulting in a sensitivity of 94% (95% CI 79% to 99%) for CT and 82% (95% CI 57% to 96%) for NG. The missed pooled samples were all derived from single-site infections with low bacterial loads. The possibility of inadequate self-sampling as a cause of false negativity was excluded, as 4/5 were collected by the physician. Testing only vaginal samples would have led to missing 40% of CT infections and 60% of NG infections. CONCLUSIONS:Pooling of samples is a cost-saving strategy for the detection of CT and NG in women, with minimal decrease in sensitivity. By reducing costs, more patients and more extragenital samples can be tested, resulting in higher detection rates.
journal_name
Sex Transm Infectjournal_title
Sexually transmitted infectionsauthors
Verougstraete N,Verbeke V,De Cannière AS,Simons C,Padalko E,Coorevits Ldoi
10.1136/sextrans-2019-054357subject
Has Abstractpub_date
2020-09-01 00:00:00pages
417-421issue
6eissn
1368-4973issn
1472-3263pii
sextrans-2019-054357journal_volume
96pub_type
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更新日期:2006-12-01 00:00:00
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journal_title:Sexually transmitted infections
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更新日期:2007-08-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
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更新日期:2008-08-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.79.3.234
更新日期:2003-06-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
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更新日期:2013-11-01 00:00:00
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1136/sextrans-2015-052014
更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1136/sti.2007.026922
更新日期:2008-02-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2018-08-01 00:00:00
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更新日期:2014-06-01 00:00:00
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doi:10.1136/sextrans-2020-054598
更新日期:2020-09-23 00:00:00
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pub_type: 杂志文章
doi:10.1136/sextrans-2015-052118
更新日期:2016-02-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2010.045104
更新日期:2010-12-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2009.040766
更新日期:2010-10-01 00:00:00
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journal_title:Sexually transmitted infections
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更新日期:2020-06-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2019-054420
更新日期:2020-07-13 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
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更新日期:2009-08-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
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journal_title:Sexually transmitted infections
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
abstract::The potential for exposure of low and high risk women to HIV and sexually transmitted infections (STI) through unprotected sex with male clients of female sex workers in Cotonou could account for most if not all of the estimated yearly numbers of HIV infections in Cotonou women (approximately 1000). As ongoing transmi...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.78.suppl_1.i69
更新日期:2002-04-01 00:00:00
abstract:OBJECTIVES:To explore the feasibility and acceptability of self-sampling for oropharyngeal and rectal specimens to screen for sexually transmitted infections (STIs) among men who have sex with men (MSM). Participant's willingness to self-sample at home was also explored. METHODS:Participants of a study to evaluate the...
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pub_type: 杂志文章
doi:10.1136/sti.2008.032193
更新日期:2009-02-01 00:00:00