Identification of lipid raft glycoproteins obtained from boar spermatozoa.

abstract：:Sulfated galactans (SGs) are highly anionic marine galactose-composed homopolysaccharides. Although their structures vary among species, their main features are conserved among phyla. Green algal SGs are quite heterogeneous, although preponderantly composed of 3-beta-D-Galp units. The red algal SGs (like agar and carr...

journal_title：Glycoconjugate journal

authors： Pomin VH

更新日期：2010-01-01 00:00:00

abstract：:Five monoclonal antibodies AS17, 22, 25, 38 and 48, a single monoclonal antibody ACH55, and three monoclonal antibodies NAH33, 43, 46, that recognize acharan sulfate (IdoA2S-GlcNAc)n, acharan (IdoA-GlcNAc)n and N-acetyl-heparosan (GlcA-GlcNAc)n, respectively, were generated by immunization of mice with keyhole limpet ...

journal_title：Glycoconjugate journal

authors： Suzuki K,Yamamoto K,Kariya Y,Maeda H,Ishimaru T,Miyaura S,Fujii M,Yusa A,Joo EJ,Kimata K,Kannagi R,Kim YS,Kyogashima M

更新日期：2008-11-01 00:00:00

abstract：:The TF, Tn, and SiaTn glycotopes are frequently expressed in cancer-associated mucins. Antibodies to these glycotopes were found in human serum. A set of polyacrylamide (PAA)--based glycoconjugates was applied to the direct and competitive enzyme-linked immunosorbent assays (ELISA) to characterize the specificity of s...

journal_title：Glycoconjugate journal

authors： Smorodin EP,Kurtenkov OA,Sergeyev BL,Pazynina GV,Bovin NV

更新日期：2004-01-01 00:00:00

abstract：:Potato lectin (Solanum tuberosum agglutinin, STA) is an unusual glycoprotein containing approximately 50% carbohydrates by weight. Of the total carbohydrates, 92% is contributed by L: -arabinose, which are O-linked to hydroxyproline residues. The ferric chloride-orcinol assay (Bial's test), which is specific for pento...

journal_title：Glycoconjugate journal

authors： Pramod SN,Venkatesh YP

更新日期：2006-11-01 00:00:00

abstract：:The interaction between Urtica dioica agglutinin (UDA) and N-acetylglucosamine (GlcNAc) and its beta(1-4)-linked oligomers was studied by fluorescence titration and isothermal titration microcalorimetry. UDA possesses one significant binding site that can be measured calorimetrically. This site is composed of three su...

journal_title：Glycoconjugate journal

authors： Lee RT,Gabius HJ,Lee YC

更新日期：1998-07-01 00:00:00

abstract：:Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...

journal_title：Glycoconjugate journal

authors： Volpi N,Zampini L,Maccari F,Santoro L,Galeotti F,Galeazzi T,Gabrielli O,Coppa GV

更新日期：2013-10-01 00:00:00

abstract：:In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45...

journal_title：Glycoconjugate journal

authors： Hernández P,Debray H,Jaekel H,Garfias Y,Jiménez Md Mdel C,Martínez-Cairo S,Zenteno E

更新日期：2001-04-01 00:00:00

abstract：:A group of fluorescent statistical glycopolymers, prepared via reversible addition-fragmentation chain-transfer (RAFT)-based polymerizations, were successfully employed in lectin-mediated bacterial binding studies. The resultant glycopolymers contained three different monomers: N-(2-hydroxyethyl) acrylamide (HEAA), N-...

journal_title：Glycoconjugate journal

authors： Wang W,Chance DL,Mossine VV,Mawhinney TP

更新日期：2014-02-01 00:00:00

abstract：:In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphi...

journal_title：Glycoconjugate journal

authors： Briggs JB,Mandrell R,Macher BA

更新日期：1996-06-01 00:00:00

abstract：:Transmannosylation from mannotriose (Man beta 1-4Man beta 1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue of N,N'-diacetylchitobiose was regioselectively induced through the use of beta-D-mannanase from Aspergillus niger. The enzyme formed the trisaccharide Man beta 1-4GlcNAc beta 1-4GlcN...

journal_title：Glycoconjugate journal

authors： Usui T,Suzuki M,Sato T,Kawagishi H,Adachi K,Sano H

更新日期：1994-04-01 00:00:00

abstract：:Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...

journal_title：Glycoconjugate journal

authors： Toyoda H,Nagai Y,Kojima A,Kinoshita-Toyoda A

更新日期：2017-04-01 00:00:00

abstract：:Two azidohexasaccharide building blocks, of which the glycosyl acceptor was the 5-(methoxycarbonyl)pentyl glycoside, were coupled using the trichloroacetimidate technology. The 12 azido functions present in the dodecasaccharide thus formed were then converted to amino groups using hydrogen sulfide as a reducing reagen...

journal_title：Glycoconjugate journal

authors： Ogawa Y,Kovac P

更新日期：1997-06-01 00:00:00

abstract：:Sialosyl-Le(x) (SLex) and its positional isomer sialosyl-Le(a) are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expr...

journal_title：Glycoconjugate journal

authors： Ito K,Handa K,Hakomori S

更新日期：1994-06-01 00:00:00

abstract：:Influenza virus neuraminidase inhibitors (NAIs), currently used as anti-influenza drugs, can lead to the appearance of drug-resistant variants. Resistance to NAIs appears due to mutations in the active site of the neuraminidase (NA) molecule that decrease the NA enzymatic activity and sometimes in the hemagglutinin (H...

journal_title：Glycoconjugate journal

authors： Mochalova L,Bright R,Xu X,Korchagina E,Chinarev A,Bovin N,Klimov A

更新日期：2010-04-01 00:00:00

abstract：:Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW approximately 32 kDa ...

journal_title：Glycoconjugate journal

authors： Raghav SK,Gupta B,Agrawal C,Saroha A,Das RH,Chaturvedi VP,Das HR

更新日期：2006-05-01 00:00:00

abstract：:Amphipathic sulpholipids have been shown to inhibit pancreatic serine proteases due to their detergent-like properties. To evaluate the structural requirement for this inhibitory activity, we examined the effects of various derivatives of sulphoglycolipids, some of which were prepared by deacylation with sphingolipid ...

journal_title：Glycoconjugate journal

authors： Suzuki H,Ito M,Kimura T,Iwamori Y,Iwamori M

更新日期：2000-11-01 00:00:00

abstract：:The structures of the branched capsular polysaccharides of group B streptococcus type III (GBSIIIPS) and Streptococcus pneumoniae type 14 (Pn14PS) are identical apart from the (α2→3)-linked sialic acid in the side chains of GBSIIIPS. The present study tries to determine the minimal epitope in GBSIIIPS, using both a pa...

journal_title：Glycoconjugate journal

authors： Safari D,Dekker HA,Rijkers GT,van der Ende A,Kamerling JP,Snippe H

更新日期：2011-12-01 00:00:00

abstract：:Mild alkaline hydrolysis of the glycophosphosphingolipids of the protozoan Leptomonas samueli liberated several phosphoinositol-containing oligosaccharides (PI-oligosaccharides), which were purified by high performance anion exchange chromatography. The oligosaccharides in the resulting four fractions were characteriz...

journal_title：Glycoconjugate journal

authors： Previato JO,Wait R,Jones C,Mendonça-Previato L

更新日期：1994-02-01 00:00:00

abstract：:The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay pro...

journal_title：Glycoconjugate journal

authors： Rizzo AM,Berra B,Rossi F,Guerra A,Gornati R,Bernardini G,Taki T,Kasama T,Mauri L,Sonnino S

更新日期：2002-01-01 00:00:00

abstract：:The specificity of the sialic acid-binding lectin from ovine placenta was examined in detail by haemagglutination inhibition assays applying a panel of 32 synthetic sialic acid analogues. The carboxylic acid group is a prerequisite for the interaction with the lectin, the alpha-anomer of the methyl glycoside is only a...

journal_title：Glycoconjugate journal

authors： Troncoso MF,Iglesias MM,Isecke R,Todel CW,Brossmer R

更新日期：2000-10-01 00:00:00

abstract：:Using printed glycan array (PGA) we compared the results of antibody profiling in undiluted, moderately (1:15) and highly (1:100) diluted human blood serum. Undiluted serum is suitable for studying blood as a tissue in its native state, whereas to study the serum of newborns or small animals one usually has to dilute ...

journal_title：Glycoconjugate journal

authors： Shilova N,Navakouski M,Khasbiullina N,Blixt O,Bovin N

更新日期：2012-04-01 00:00:00

abstract：:Duffy antigen/receptor for chemokines (DARC) is a glycosylated seven-transmembrane protein acting as a blood group antigen, a chemokine binding protein and a receptor for Plasmodium vivax malaria parasite. It is present on erythrocytes and endothelial cells of postcapillary venules. The N-terminal extracellular domain...

journal_title：Glycoconjugate journal

authors： Grodecka M,Bertrand O,Karolak E,Lisowski M,Waśniowska K

更新日期：2012-04-01 00:00:00

abstract：:Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

journal_title：Glycoconjugate journal

authors： Christensen LL,Bross P,Ørntoft TF

更新日期：2000-12-01 00:00:00

abstract：:Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A numbe...

journal_title：Glycoconjugate journal

authors： Nemčovičová I,Šesták S,Rendić D,Plšková M,Mucha J,Wilson IB

更新日期：2013-12-01 00:00:00

abstract：:N-acetylglucosaminyltransferase (GnT)-IV a is a key enzyme that catalyzes the formation of the GlcNAC β1-4 branch on the core structure of complex N-Glycans, which is the common substrate for other N-acetylglucosaminyltransferases, such as GnT-III and GnT-V. Our recent study indicates that the expression of GnT-IVa in...

journal_title：Glycoconjugate journal

authors： Fan J,Wang S,Yu S,He J,Zheng W,Zhang J

更新日期：2012-08-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:Fucosylated oligosaccharides have an important role in maintaining a healthy immune system and homeostatic gut microflora. This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. The production was optimized using experiment desig...

journal_title：Glycoconjugate journal

authors： Usvalampi A,Maaheimo H,Tossavainen O,Frey AD

更新日期：2018-02-01 00:00:00

abstract：:The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J 5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoprotei...

journal_title：Glycoconjugate journal

authors： Altmann F

更新日期：1998-01-01 00:00:00

abstract：:A straight forward synthesis of a heptasaccharide part of the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990, known to have antigenicity, has been achieved in excellent yield. Judicious choice of protecting groups in the intermediates played a significant role throughout the synthesis. Most of the i...

journal_title：Glycoconjugate journal

authors： Panchadhayee R,Kumar Misra A

更新日期：2008-12-01 00:00:00

abstract：:Our recent studies have revealed the existence of two distinct Gal: 3-O-sulfotransferases capable of acting on the C-3 position of galactose in a Core 2 branched structure, e.g., Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNacalph a1-->OBenzyl as acceptor to give 3-O-sulfoGalbeta1-->4GlcNAcbeta1-->3(Galbeta1-->3)G alN...

journal_title：Glycoconjugate journal

authors： Jain RK,Piskorz CF,Chandrasekaran EV,Matta KL

更新日期：1998-10-01 00:00:00