Identification of lipid raft glycoproteins obtained from boar spermatozoa.

abstract：:Synthesis and antigenic reactivity of 6-O-sulfo sialylparagloboside (SPG) and sialyl Lewis X (sLeX) neo-glycolipids containing lactamized neuraminic acid are described. The suitably protected GlcNAc-beta (1-->3)-Gal-beta (1-->4)-GlcOSE derivative was glycosylated with NeuTFAc-alpha (2-->3)-Gal imidate to give NeuTFAc-...

journal_title：Glycoconjugate journal

authors： Yamaguchi M,Ishida H,Kanamori A,Kannagi R,Kiso M

更新日期：2005-03-01 00:00:00

abstract：:Incubation of synthetic Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe, and GlcNAc beta 1-4GlcNAc beta-OMe with CMP-Neu5Ac and rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Ac alpha 2-6Man beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6Ga...

journal_title：Glycoconjugate journal

authors： Hokke CH,van der Ven JG,Kamerling JP,Vliegenthart JF

更新日期：1993-02-01 00:00:00

abstract：:In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45...

journal_title：Glycoconjugate journal

authors： Hernández P,Debray H,Jaekel H,Garfias Y,Jiménez Md Mdel C,Martínez-Cairo S,Zenteno E

更新日期：2001-04-01 00:00:00

abstract：:Human β-1,4-galactosyltransferase (β-1,4-GalT) V was shown to be involved in the biosynthesis of N-glycans, O-glycans and lactosylceramide (Lac-Cer) by in vitro studies. To determine its substrate specificity, enzymatic activity and its products were analyzed using mouse embryonic fibroblast (MEF) cells from β-1,4-Gal...

journal_title：Glycoconjugate journal

authors： Kumagai T,Sato T,Natsuka S,Kobayashi Y,Zhou D,Shinkai T,Hayakawa S,Furukawa K

更新日期：2010-10-01 00:00:00

abstract：:The N-glycans from soluble extracts of ten pollens were examined. The pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase HPLC, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. In compar...

journal_title：Glycoconjugate journal

authors： Wilson IB,Altmann F

更新日期：1998-11-01 00:00:00

abstract：:Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...

journal_title：Glycoconjugate journal

authors： Suresh K,Vegiraju SR,Nadimpalli SK

更新日期：2004-01-01 00:00:00

abstract：:Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized be...

journal_title：Glycoconjugate journal

authors： Sauerzapfe B,Krenek K,Schmiedel J,Wakarchuk WW,Pelantová H,Kren V,Elling L

更新日期：2009-02-01 00:00:00

abstract：:The structures of the branched capsular polysaccharides of group B streptococcus type III (GBSIIIPS) and Streptococcus pneumoniae type 14 (Pn14PS) are identical apart from the (α2→3)-linked sialic acid in the side chains of GBSIIIPS. The present study tries to determine the minimal epitope in GBSIIIPS, using both a pa...

journal_title：Glycoconjugate journal

authors： Safari D,Dekker HA,Rijkers GT,van der Ende A,Kamerling JP,Snippe H

更新日期：2011-12-01 00:00:00

abstract：:Fucosylated oligosaccharides have an important role in maintaining a healthy immune system and homeostatic gut microflora. This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. The production was optimized using experiment desig...

journal_title：Glycoconjugate journal

authors： Usvalampi A,Maaheimo H,Tossavainen O,Frey AD

更新日期：2018-02-01 00:00:00

abstract：:Since the alpha-D-galactose-(1-->3)-D-galactose epitope has been identified to be the major target in the process of hyperacute rejection of xenografts transplanted from nonprimate donors to humans, specific inhibitors of alpha-galactosyltransferases are of broad interest. Using Trypanosoma brucei, a protozoan parasit...

journal_title：Glycoconjugate journal

authors： Kolb V,Amann F,Schmidt RR,Duszenko M

更新日期：1999-09-01 00:00:00

abstract：:A group of fluorescent statistical glycopolymers, prepared via reversible addition-fragmentation chain-transfer (RAFT)-based polymerizations, were successfully employed in lectin-mediated bacterial binding studies. The resultant glycopolymers contained three different monomers: N-(2-hydroxyethyl) acrylamide (HEAA), N-...

journal_title：Glycoconjugate journal

authors： Wang W,Chance DL,Mossine VV,Mawhinney TP

更新日期：2014-02-01 00:00:00

abstract：:Cultured chondrocytes derived from the caudal and cephalic ends of embryonic chick sterna have been compared with each other and with whole sternum, by using a panel of 21 lectins to probe the distribution of oligosaccharides in glycoconjugates of cells and matrix at various times of culture or development. On culture...

journal_title：Glycoconjugate journal

authors： McClure SF,Stoddart RW,McClure J

更新日期：1997-04-01 00:00:00

abstract：:Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describ...

journal_title：Glycoconjugate journal

authors： Axelsson MA,Hansson EM,Sikut R,Hansson GC

更新日期：1998-08-01 00:00:00

abstract：:Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...

journal_title：Glycoconjugate journal

authors： Volpi N,Zampini L,Maccari F,Santoro L,Galeotti F,Galeazzi T,Gabrielli O,Coppa GV

更新日期：2013-10-01 00:00:00

abstract：:Galectin-1 has demonstrated a diverse range of activities in relation to cell survival and proliferation. In different circumstances, it acts as a mitogen, as an inhibitor of cell proliferation, and as a promoter of cellular apoptosis. Many of these activities, particularly the mitogenic and apoptotic responses, follo...

journal_title：Glycoconjugate journal

authors： Scott K,Weinberg C

更新日期：2002-01-01 00:00:00

abstract：:The sugar chain-binding specificity of tomato lectin (LEA) against glycoproteins was investigated qualitatively using lectin blot analysis. Glycoproteins containing tri- and tetra-antennary complex-type N-glycans were stained with LEA. Unexpectedly, glycoproteins containing high mannose-type N-glycans and a horseradis...

journal_title：Glycoconjugate journal

authors： Oguri S

更新日期：2005-11-01 00:00:00

abstract：:The TF, Tn, and SiaTn glycotopes are frequently expressed in cancer-associated mucins. Antibodies to these glycotopes were found in human serum. A set of polyacrylamide (PAA)--based glycoconjugates was applied to the direct and competitive enzyme-linked immunosorbent assays (ELISA) to characterize the specificity of s...

journal_title：Glycoconjugate journal

authors： Smorodin EP,Kurtenkov OA,Sergeyev BL,Pazynina GV,Bovin NV

更新日期：2004-01-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids. The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients wi...

journal_title：Glycoconjugate journal

authors： Sturiale L,Bianca S,Garozzo D,Terracciano A,Agolini E,Messina A,Palmigiano A,Esposito F,Barone C,Novelli A,Fiumara A,Jaeken J,Barone R

更新日期：2019-12-01 00:00:00

abstract：:The complex microenvironment that surrounds hematopoietic stem cells (HSCs) in the bone marrow niche involves different coordinated signaling pathways. The stem cells establish permanent interactions with distinct cell types such as mesenchymal stromal cells, osteoblasts, osteoclasts or endothelial cells and with secr...

journal_title：Glycoconjugate journal

authors： Papy-Garcia D,Albanese P

更新日期：2017-06-01 00:00:00

abstract：:Glycosylation is a very important post-translational modification involved in various cellular processes, such as cell adhesion, signal transduction and immune response. Urine is a rich source of glycoproteins and attractive biological fluid for biomarker discovery, owing to its availability, ease of collection, and c...

journal_title：Glycoconjugate journal

authors： Kawahara R,Saad J,Angeli CB,Palmisano G

更新日期：2016-12-01 00:00:00

abstract：:Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family. Syndecan-4-deficient [(Synd4(-/-)] mice were produced to clarify the in vivo role of syndecan-4. Synd4(-/-) mice were more susceptible to kappa-carrageenan-induced nephropathy, and the placental labyrinth from the deficient em...

journal_title：Glycoconjugate journal

authors： Ishiguro K,Kojima T,Muramatsu T

更新日期：2002-05-01 00:00:00

abstract：:Mammalian sulfoglycolipids are comprised of two major members, sulfatide (SO(3)-3Gal-ceramide) and seminolipid (SO(3)-3Gal-alkylacylglycerol). Sulfatide is abundant in the myelin sheath and seminolipid is expressed on the spermatogenic cells. Cerebroside sulfotransferase (CST)-deficient mice generated by gene targetin...

journal_title：Glycoconjugate journal

authors： Honke K,Zhang Y,Cheng X,Kotani N,Taniguchi N

更新日期：2004-01-01 00:00:00

abstract：:Abnormal glycosylation of proteins is known to be either resultant or causative of a variety of diseases. This makes glycoproteins appealing targets as potential biomarkers and focal points of molecular studies on the development and progression of human ailment. To date, a majority of efforts in disease glycoproteomi...

journal_title：Glycoconjugate journal

authors： Schumacher KN,Dodds ED

更新日期：2016-06-01 00:00:00

abstract：:Jacalin and M. pomifera agglutinin are T-antigen specific lectins with alpha 4 beta 4 structures that show far greater micro-heterogeneity than plant lectins from other families, due to multiple genetic isoforms and post-translational processing. Electrospray mass spectrometry and combined liquid chromatography-electr...

journal_title：Glycoconjugate journal

authors： Young NM,Watson DC,Thibault P

更新日期：1995-04-01 00:00:00

abstract：:The specificity of the sialic acid-binding lectin from ovine placenta was examined in detail by haemagglutination inhibition assays applying a panel of 32 synthetic sialic acid analogues. The carboxylic acid group is a prerequisite for the interaction with the lectin, the alpha-anomer of the methyl glycoside is only a...

journal_title：Glycoconjugate journal

authors： Troncoso MF,Iglesias MM,Isecke R,Todel CW,Brossmer R

更新日期：2000-10-01 00:00:00

abstract：:Diabetic mellitus is attended by the development of endothelial dysfunction which is suggested to be accompanied with a chronic low-degree of inflammation. During a chronic hepatic inflammatory response, specific changes in glycosylation of the acute phase protein alpha1-acid glycoprotein (AGP) can be detected. In thi...

journal_title：Glycoconjugate journal

authors： Poland DC,Schalkwijk CG,Stehouwer CD,Koeleman CA,van het Hof B,van Dijk W

更新日期：2001-03-01 00:00:00

abstract：:The conformational space of methyl 6-O-[(R)- and (S)-1-carboxyethyl]-alpha-D-galactopyranoside has been investigated. A grid search employing energy minimization at each grid point over the three major degrees of freedom, namely phi, psi and omega, identified low energy regions. The R-isomer shows five low energy conf...

journal_title：Glycoconjugate journal

authors： Stenutz R,Widmalm G

更新日期：1997-12-01 00:00:00

abstract：:Nucleic acids bearing glycans of various structures have been under vigorous investigation in the past decade. The carbohydrate moieties of such complexes can serve as recognition sites for carbohydrate-binding proteins-lectins-and initiate receptor-mediated endocytosis. Therefore, carbohydrates can enhance cell targe...

journal_title：Glycoconjugate journal

authors： Yan H,Tram K

更新日期：2007-04-01 00:00:00

abstract：:To elucidate the mechanism underlying the hydrolysis of the GalNAcbeta1-->4Gal linkage in ganglioside GM2 [GalNAcbeta1-->4(NeuAcalpha2-->3)Galbeta1-->4Glcbeta1-->1' Cer] by beta-hexosaminidase A (Hex A) with GM2 activator protein, we designed and synthesized two kinds of GM2 linkage analogues-6'-NeuAc-GM2 and alpha-Ga...

journal_title：Glycoconjugate journal

authors： Komori T,Ando T,Imamura A,Li YT,Ishida H,Kiso M

更新日期：2008-10-01 00:00:00

abstract：:Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, n...

journal_title：Glycoconjugate journal

authors： Mizumoto S,Fongmoon D,Sugahara K

更新日期：2013-08-01 00:00:00