Imaging single mRNA molecules in yeast.

Abstract:

:Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells.

journal_name

Methods Enzymol

journal_title

Methods in enzymology

authors

Youk H,Raj A,van Oudenaarden A

doi

10.1016/S0076-6879(10)70017-3

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

429-46

eissn

0076-6879

issn

1557-7988

pii

S0076-6879(10)70017-3

journal_volume

470

pub_type

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