Abstract:
:Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Youk H,Raj A,van Oudenaarden Adoi
10.1016/S0076-6879(10)70017-3subject
Has Abstractpub_date
2010-01-01 00:00:00pages
429-46eissn
0076-6879issn
1557-7988pii
S0076-6879(10)70017-3journal_volume
470pub_type
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