Abstract:
:Kaposi's sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman's disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). A protein of about 27 kDa was expressed as expected. Western Blotting showed that the purified recombinant ORF73 reacted with KSHV positive serum. The immunogenicity of the recombinant ORF73 was further analysed by ELISA and the optimal conditions were determined. The ORF73 ELISA was used to compare the KSHV seroprevalence between Hubei and Xinjiang Han people. The Han people in Xinjiang have significantly higher KSHV seroprevalence than their counterparts in Hubei (6.7% vs 2.9%, P = 0.005).
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Ouyang XX,Fu BS,Li BL,Zeng Y,Xu FH,Wang LDdoi
10.1007/s12250-010-3106-4subject
Has Abstractpub_date
2010-06-01 00:00:00pages
168-76issue
3eissn
1674-0769issn
1995-820Xjournal_volume
25pub_type
杂志文章abstract::Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the n...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3239-8
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journal_title:Virologica Sinica
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doi:10.1007/s12250-011-3196-7
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journal_title:Virologica Sinica
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doi:10.1007/s12250-019-00134-z
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journal_title:Virologica Sinica
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doi:10.1007/s12250-017-4077-5
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journal_title:Virologica Sinica
pub_type: 杂志文章,评审
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journal_title:Virologica Sinica
pub_type: 杂志文章,评审
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journal_title:Virologica Sinica
pub_type: 杂志文章,评审
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journal_title:Virologica Sinica
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doi:10.1007/s12250-013-3348-z
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journal_title:Virologica Sinica
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更新日期:2011-12-01 00:00:00
abstract::Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression ...
journal_title:Virologica Sinica
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3750-4
更新日期:2016-08-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章,meta分析
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更新日期:2015-10-01 00:00:00
abstract::Ebola virus (EBOV) and Marburg virus (MARV), belonging to the Filoviridae family, emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates. As high as 50-90% mortality, filoviruses can cause significant threats to public health. However, so far no specific and efficient vaccine ha...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3186-9
更新日期:2011-04-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3997-4
更新日期:2017-06-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00087-3
更新日期:2019-02-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3234-0
更新日期:2012-04-01 00:00:00
abstract::Recreational waters contaminated with human fecal pollution are a public health concern, and ensuring the safety of recreational waters for public use is a priority of both the Environmental Protection Agency (EPA) and the Centers for Disease Control and Prevention (CDC). Current recreational water standards rely on f...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-015-3644-x
更新日期:2015-10-01 00:00:00
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journal_title:Virologica Sinica
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doi:10.1007/s12250-012-3249-6
更新日期:2012-08-01 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...
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abstract::Rotavirus (RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens (HBGAs). Previously, the protruding domain protein (P protein)...
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abstract::Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication...
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abstract::An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...
journal_title:Virologica Sinica
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更新日期:2016-08-01 00:00:00
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更新日期:2020-09-22 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00138-9
更新日期:2019-10-01 00:00:00
abstract::Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provid...
journal_title:Virologica Sinica
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doi:10.1007/s12250-012-3252-y
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