Abstract：
:Oil-frozen water-in-oil-in-water (W₁/O/W₂) double emulsions (DE) containing ethanol up to 40% (w/v) in the external aqueous W₂ phase exhibited external coalescence upon thawing of the oil phase, releasing up to 85% of the encapsulated protein of the internal aqueous phase. These emulsions were studied in vitro as potential dermal macromolecular delivery formulations, achieving fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) penetration of up to 86 μm into porcine skin, reaching the viable epidermis where the immunocompetent Langerhans cells are located. Enzyme-linked immunosorbent assay was performed to observe the effect of the emulsification process and ethanol content on the ability of BSA to form antigen-antibody complexes; results indicated that ethanol content and the emulsification process did not diminish the BSA-antibody complex formation when compared with a BSA standard aqueous solution. Therefore, it is shown that oil-frozen W₁/O/W₂ DE, with penetration-enhancing ethanol in the W₂ phase, can potentially be used for cutaneous vaccine delivery formulations.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Jaimes-Lizcano YA,Lawson LB,Papadopoulos KDdoi
10.1002/jps.22362subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1398-406issue
4eissn
0022-3549issn
1520-6017pii
S0022-3549(15)32196-1journal_volume
100pub_type
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