Abstract:
:The sophistication of intracellular Ca ( 2+) signalling reflects the necessity for the many different types of cells to fine tuning their specific activities. This can, at least in part, be explained by the molecular complexity of the Ca ( 2+) signalling machinery, consisting of different intracellular Ca ( 2+) release channel types, each including multiple isoforms and alternative splicing variants. This commentary will go over the main points concerning expression and functional characterization of alternative splicing variants of inositol 1,4,5-trisphosphate and ryanodine receptor isoforms. Many of these variants display specific activation or regulatory features. In addition, dominant negative effects of non-functional alternative splicing variants have been also described for both InsP3Rs and RyRs channels. Recently, a novel RyR2 transcript has been identified by Takasawa and co-workers in pancreatic islets. This novel RyR2 transcript has been proposed to act as an intracellular target for cADPR signalling, which has been demonstrated to be important for insulin secretion. Future characterization of this RyR2 transcript may represent a significant advancement in understanding the mechanisms underlying regulation of Ca ( 2+) release by cADPR.
journal_name
Isletsjournal_title
Isletsauthors
Rossi D,Sorrentino Vdoi
10.4161/isl.2.6.13859subject
Has Abstractpub_date
2010-11-01 00:00:00pages
383-5issue
6eissn
1938-2014issn
1938-2022pii
13859journal_volume
2pub_type
杂志文章相关文献
Islets文献大全abstract::NeuroD (BETA2), a bHLH transcription factor is known to be involved in early pancreatic endocrine as well as neuronal differentiation. NeuroD is known to regulate distinct sets of molecules that are important for survival and differentiation of pancreatic cells. We assessed whether forced expression of NeuroD, can ind...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.1.1.8993
更新日期:2009-07-01 00:00:00
abstract::Islet transplantation is a promising treatment for Type 1 diabetes; however limitations of the intra-portal site and poor revascularization of islets must be overcome. We hypothesize that engineering a highly vascularized collagen-based construct will allow islet graft survival and function in alternative sites. In th...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.27175
更新日期:2013-09-01 00:00:00
abstract::Inactivation of the gene encoding the aristaless-related homeodomain transcription factor Alx3 results in islet cell apoptosis and impaired glucose homeostasis that worsens with age due to the appearance of insulin resistance. Alx3-deficient mice also show extrapancreatic developmental defects with variable penetrance...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.3.2.14658
更新日期:2011-03-01 00:00:00
abstract::The members of the Suppressor of Cytokine Signaling (SOCS) protein family mainly modulate the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. SOCS-1 and SOCS-3 have already been shown to influence growth and apoptosis of pancreatic beta cells. We hypothesized that SOCS-2, which is e...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.2.4.12556
更新日期:2010-07-01 00:00:00
abstract::Several lines of recent evidence provided compelling evidence to suggest increased generation of reactive oxygen species (ROS) as causal for mitochondrial dysregulation and apoptosis in islet β-cells exposed to noxious stimuli including high glucose, lipids and proinflammatory cytokines. Studies along these lines are ...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.25058
更新日期:2013-05-01 00:00:00
abstract::BACKGROUND: Embolic occlusion of the portal vein due to islet transplantation is one of the major reasons for reduced survival of transplanted islets. In this study, we examined the location of islets as well as the correlation between islet and portal vein size after intraportal islet transplantation and evaluated li...
journal_title:Islets
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::Histopathology based studies of the pancreas obtained from organ donors are increasing our awareness of islet phenotypic heterogeneity during development and aging, as well as in settings of type 1 diabetes, type 2 diabetes, monogenic diabetes or other forms of this metabolic disease. Islet amyloidosis represents a hi...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2019.1599707
更新日期:2019-01-01 00:00:00
abstract::Members of the TGFß superfamily, including activins and TGFß, modulate glucose-stimulated insulin secretion (GSIS) in vitro using rat islets while genetic manipulations that reduce TGFß superfamily signaling in vivo in mice produced hypoplastic islets and/or hyperglycemia. Moreover, deletion of Fstl3, an antagonist of...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.3.6.18013
更新日期:2011-11-01 00:00:00
abstract::The Reg3 peptides INGAP-PP and human Reg3α/β (HIP) have been hypothesized to stimulate β-cell neogenesis in the pancreas. Administration of INGAP-PP has been shown to cause an increase in β-cell mass in multiple animal models, reverse streptozotocin (STZ) induced diabetes in mice and reduces HbA1c levels in type 2 dia...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.18659
更新日期:2012-01-01 00:00:00
abstract::We have previously identified two genes, encoding lactate dehydrogenase (Ldha) and the monocarboxylate carrier, MCT1 (Slc16a1) whose expression is remarkably low in pancreatic β-cells and islets. We sought here to determine whether these may be part of a larger family of genes selectively repressed ("disallowed") in t...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.2.2.11025
更新日期:2010-03-01 00:00:00
abstract::We recently reported that human blood outgrowth endothelial cells (BOEC) are supportive to reverse hyperglycemia in marginal islet mass-transplanted diabetic mice. In this report, we investigated whether the observed effect was evoked by islet packing in a blood clot prior to transplantation or could be mimicked by an...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.26778
更新日期:2013-09-01 00:00:00
abstract::Insulin is secreted from the pancreatic β-cells in response to elevated glucose. In intact islets the capacity for insulin release is determined by a complex interplay between different cell types. This has made it difficult to specifically assess the role of β-cell defects to the insulin secretory impairment in type ...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.28095
更新日期:2014-01-01 00:00:00
abstract::Metabolic syndrome (MS) can be defined as a group of signs that increases the risk of developing type 2 diabetes mellitus (DM2). These signs include obesity, hyperinsulinemia and insulin resistance. We are interested in the mechanisms that trigger hyperinsulinemia as a step to understand how β cells fail in DM2. Pancr...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.21374
更新日期:2012-07-01 00:00:00
abstract::A potentiating effect of medium-chain triglycerides on glucose-stimulated insulin secretion (GSIS) has been observed since the 1960s. Subsequent observations identified octanoic acid (OA), the main component of medium-chain triglyceride, as the potentiator of GSIS, but the mechanism was unclear. We used wild-type (WT)...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2019.1566683
更新日期:2019-01-01 00:00:00
abstract::The ability of the β-cells to control blood glucose levels depends on their function and mass. In both, type 1 and type 2 diabetes mellitus the main processes leading to β-cell failure are apoptosis and loss of function. Many studies demonstrate how cytokines and chemokines have an active role in triggering the immune...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.1.3.9110
更新日期:2009-11-01 00:00:00
abstract::Type 1 and type 2 diabetes are ultimately characterized by depleted β-cell mass. Characterization of the molecular pathways that control β-cell proliferation could be harnessed to restore these cells. The homeobox β-cell transcription factor Nkx6.1 induces β-cell proliferation by activating the orphan nuclear receptor...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2015.1027854
更新日期:2015-01-01 00:00:00
abstract::Worldwide efforts are underway to replace or repair lost or dysfunctional pancreatic β-cells to cure diabetes. However, it is unclear what the final product of these efforts should be, as β-cells are thought to be heterogeneous. To enable the analysis of β-cell heterogeneity in an unbiased and quantitative way, we dev...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2016.1150664
更新日期:2016-03-03 00:00:00
abstract::The total mass of pancreatic islet cells is a critical factor in glucose metabolic control. The aim of the present study was to investigate whether in the Munich Wistar Frömter (MWF) rat, beside a reduction in the number of nephrons, there are also alterations in the number of pancreatic islets and of β cell mass. We ...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.2.5.13178
更新日期:2010-09-01 00:00:00
abstract::Assessing the response of pancreatic islet cells to glucose stimulation is important for understanding β-cell function. Zebrafish are a promising model for studies of metabolism in general, including stimulus-secretion coupling in the pancreas. We used transgenic zebrafish embryos expressing a genetically-encoded Ca2+...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2018.1540234
更新日期:2018-01-01 00:00:00
abstract::The transcription factor Pancreatic and Duodenal Homeobox-1 (PDX-1) plays a major role in the development and function of pancreatic β-cells and its mutation results in diabetes. In adult β-cells, glucose stimulates transcription of the insulin gene in part by regulating PDX-1 expression, stability and activity. Gluco...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/19382014.2014.982376
更新日期:2014-01-01 00:00:00
abstract::The purification process is one of the most difficult procedures in pancreatic islet isolation. It was demonstrated that the standard purification method using a COBE 2991 cell processor with Ficoll density gradient solution harmed islets mechanically by high shear force. We reported that purification using large bott...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.23008
更新日期:2012-11-01 00:00:00
abstract::Diabetes (T1DM and T2DM) is characterized by a deficit in β-cell mass. A broader understanding of human β-cell replication mechanism is thus important to increase β-cell proliferation for future therapeutic interventions. Here, we show that p27 (Kip1), a CDK inhibitor, is expressed abundantly in isolated adult human i...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.3.1.14435
更新日期:2011-01-01 00:00:00
abstract::Clinical islet transplantation has routinely been demonstrated to be an efficacious means of restoring glycemic control in select patients with autoimmune diabetes. Notwithstanding marked progress and improvements, the broad-spectrum application of this treatment option is restricted by the complications associated wi...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2016.1253652
更新日期:2016-11-01 00:00:00
abstract::Islet replacement is a promising approach for type-1 diabetes treatment, but the shortage of organ donors demands new sources of β-cells. The use of stem/precursor cells may represent an attractive alternative. Islet-derived stem/precursor cells (hIPC) have been isolated from human islet preparations, but neither thei...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.2.3.11449
更新日期:2010-05-01 00:00:00
abstract::A growing body of evidence implicates essential roles for small molecular weight G-proteins (e.g., Cdc42, Rac1, Arf6 and Rab3A and Rab27A) in islet β-cell function including glucose-stimulated insulin secretion (GSIS). One of the known mechanisms for optimal activation of small G-proteins involves post-translational p...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.22538
更新日期:2012-09-01 00:00:00
abstract::A 39-y-old man, who had an episode of pancreatic bleeding due to chronic pancreatitis, received total pancreatectomy with islet autotransplantation (TP with IAT). Intraoperative ultrasound (US) examination was done to detect transplanted islets and evaluate the quality of US imaging. Islet isolation from the resecte...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.22384
更新日期:2012-09-01 00:00:00
abstract::Zinc transporter 8 (ZnT8) has been identified as a β-cell-specific Zinc transporter expressed in insulin-secretory granules. Recent genome wide association studies indicated that Arg325Trp polymorphism of Slc30a8 encoding ZnT8 is associated with susceptibility to type 2 diabetes. As a first step towards understanding ...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.1.2.9433
更新日期:2009-09-01 00:00:00
abstract::Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2018.1460294
更新日期:2018-05-04 00:00:00
abstract:UNLABELLED:AIMS/ HYPOTHESIS: Caspase-3 is a main effector caspase of the apoptotic cascade. Involvement of caspase-3 has been implicated in a β cell cloned cell line from type 1 diabetic subjects and in isolated islets from type 2 diabetic subjects. RESULTS:The control islets revealed cleaved caspase-3 positive cells ...
journal_title:Islets
pub_type: 杂志文章
doi:10.4161/isl.2.1.10041
更新日期:2010-01-01 00:00:00
abstract::The endocrine pancreas expands markedly in the first postnatal days and the insulin producing β-cells initiate a functional maturation preceded by a morphological change of the islets of Langerhans. Trefoil factor 3 (TFF3) is a secreted peptide expressed in intestinal epithelia, where it promotes migration, but its ro...
journal_title:Islets
pub_type: 杂志文章
doi:10.1080/19382014.2018.1472186
更新日期:2018-05-04 00:00:00