How Cells feel their environment: a focus on early dynamic events.

abstract：:The delivery of therapeutics to the central nervous system (CNS) remains a major challenge in part due to the presence of the blood-brain barrier (BBB). Recently, cell-derived vesicles, particularly exosomes, have emerged as an attractive vehicle for targeting drugs to the brain, but whether or how they cross the BBB ...

journal_title：Cellular and molecular bioengineering

authors： Chen CC,Liu L,Ma F,Wong CW,Guo XE,Chacko JV,Farhoodi HP,Zhang SX,Zimak J,Ségaliny A,Riazifar M,Pham V,Digman MA,Pone EJ,Zhao W

更新日期：2016-12-01 00:00:00

abstract：:Advances in the use of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for heart regeneration and in vitro disease models demand a greater understanding of how these cells grow and mature in 3-dimensional space. In this study, we developed an analysis methodology of single cardiomyocytes plated on 2...

journal_title：Cellular and molecular bioengineering

authors： Rupert CE,Chang HH,Coulombe KL

更新日期：2017-02-01 00:00:00

abstract：:A fundamental question in mechanobiology is how mechanical stimuli are sensed by mechanosensing proteins and converted into signals that direct cells to adapt to the external environment. A key function of cell adhesion to the extracellular matrix (ECM) is to transduce mechanical forces between cells and their extrace...

journal_title：Cellular and molecular bioengineering

authors： Yan J,Yao M,Goult BT,Sheetz MP

更新日期：2015-01-01 00:00:00

abstract：:Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell ...

journal_title：Cellular and molecular bioengineering

authors： Dumbleton J,Agarwal P,Huang H,Hogrebe N,Han R,Gooch KJ,He X

更新日期：2016-06-01 00:00:00

abstract：:Actomyosin kinetics in both skinned skeletal muscle fibers at maximum Ca2+-activation and unregulated in vitro motility assays are modulated by solvent microviscosity in a manner consistent with a diffusion limited process. Viscosity might also influence cardiac thin filament Ca2+-regulatory protein dynamics. In vitro...

journal_title：Cellular and molecular bioengineering

authors： Loong CK,Takeda AK,Badr MA,Rogers JS,Chase PB

更新日期：2013-06-01 00:00:00

abstract：:Blood flow-associated shear stress causes physiological and pathophysiological biochemical processes in endothelial cells that may be initiated by alterations in plasma membrane lipid domains characterized as liquid-ordered (l(o)), such as rafts or caveolae, or liquid-disordered (l(d)). To test for domain-dependent sh...

journal_title：Cellular and molecular bioengineering

authors： Tabouillot T,Muddana HS,Butler PJ

更新日期：2011-06-01 00:00:00

abstract：:Thermoresponsive polymers are being used increasingly in cell culture applications due to their temperature dependent surface properties. Poly(MEO2MA-co-OEGMA) (PMO) brushes offer tunable physical properties via variation in the copolymer ratio, but the effects of composition on cell-substrate interactions is unclear....

journal_title：Cellular and molecular bioengineering

authors： Anderson CR,Abecunas C,Warrener M,Laschewsky A,Wischerhoff E

更新日期：2016-08-24 00:00:00

abstract：Introduction:Immune checkpoint inhibitors that boost cytotoxic T cell-based immune responses have emerged as one of the most promising approaches in cancer treatment. However, it is increasingly being realized that T cell activation needs to be rationally combined with molecularly targeted therapeutics for a maximal an...

journal_title：Cellular and molecular bioengineering

authors： Ramesh A,Natarajan SK,Nandi D,Kulkarni A

更新日期：2019-05-21 00:00:00

abstract：:The cell nucleus is becoming increasingly recognized as a mechanosensitive organelle. Most research on nuclear mechanosignaling focuses on the nuclear lamina and coupled actin structures. In this commentary, we discuss the possibility that the nuclear membrane senses and transduces mechanical signals similar to the pl...

journal_title：Cellular and molecular bioengineering

authors： Enyedi B,Niethammer P

更新日期：2016-06-01 00:00:00

abstract：Introduction:Multicellular platforms and linked multi organ on chip devices are powerful tools for drug discovery, and basic mechanistic studies. Often, a critical constraint is defining a culture medium optimal for all cells present in the system. In this study, we focused on the key cells of the neuromuscular junctio...

journal_title：Cellular and molecular bioengineering

authors： Besser RR,Bowles AC,Alassaf A,Carbonero D,Maciel R,Saporta M,Agarwal A

更新日期：2020-06-10 00:00:00

abstract：Introduction:The orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires in vitro assa...

journal_title：Cellular and molecular bioengineering

authors： Mohammed D,Pardon G,Versaevel M,Bruyère C,Alaimo L,Luciano M,Vercruysse E,Pruitt BL,Gabriele S

更新日期：2019-09-25 00:00:00

abstract：:Chlamydia pneumoniae is a common respiratory pathogen that is considered a highly likely risk factor for atherosclerosis. C. pneumoniae is disseminated from the lung into systemic circulation via infected monocytes and lodges at the atherosclerotic sites. During transit, C. pneumoniae-infected monocytes in circulation...

journal_title：Cellular and molecular bioengineering

authors： Evani SJ,Dallo SF,Murthy AK,Ramasubramanian AK

更新日期：2013-09-01 00:00:00

abstract：Introduction:The propagation of mechanochemical signals from the extracellular matrix to the cell nucleus has emerged as a central feature in regulating cellular differentiation and de-differentiation. This process of outside-in signaling and the associated mechanotransduction pathways have been well described in numer...

journal_title：Cellular and molecular bioengineering

authors： Li Y,Tang CB,Kilian KA

更新日期：2017-07-18 00:00:00

abstract：Introduction:Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown. Methods:FSS (6 dyn/cm2) was produced in a flow chamber. C...

journal_title：Cellular and molecular bioengineering

authors： Zheng L,Shi Q,Na J,Liu N,Guo Y,Fan Y

更新日期：2018-08-02 00:00:00

abstract：:The generation of stable intracellular concentration gradients is a useful method for local control of cell function, selective manipulation of cellular structures and testing hypotheses related to dynamical intracellular processes. Cell culture in a microfluidic device allows the presentation of a stable gradient of ...

journal_title：Cellular and molecular bioengineering

authors： Aggarwal V,Lele TP

更新日期：2016-12-16 00:00:00

abstract：Introduction:The mechanical response of large multi-cellular collectives to external stretch has remained largely unexplored, despite its relevance to normal function and to external challenges faced by some tissues. Here, we introduced a simple hybrid silicone substrate to enable external stretch while providing a phy...

journal_title：Cellular and molecular bioengineering

authors： Bashirzadeh Y,Dumbali S,Qian S,Maruthamuthu V

更新日期：2019-02-01 00:00:00

abstract：:Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image veloci...

journal_title：Cellular and molecular bioengineering

authors： Nève N,Kohles SS,Winn SR,Tretheway DC

更新日期：2010-09-01 00:00:00

abstract：:In developing implantable tissues based on cellular remodeling of a fibrin scaffold, a key indicator of success is high collagen content. Cellular collagen synthesis is stimulated by cyclic stretching but is limited by cellular adaptation. Adaptation is mediated by deactivation of extracellular signal-regulated kinase...

journal_title：Cellular and molecular bioengineering

authors： Weinbaum JS,Schmidt JB,Tranquillo RT

更新日期：2013-09-01 00:00:00

abstract：:In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Han...

journal_title：Cellular and molecular bioengineering

authors： Huo B,Lu XL,Hung CT,Costa KD,Xu Q,Whitesides GM,Guo XE

更新日期：2008-03-01 00:00:00

abstract：:The biomechanical properties of living cells depend on their molecular building blocks, and are important for maintaining structure and function in cells, the extracellular matrix, and tissues. These biomechanical properties and forces also shape and modify the cellular and extracellular structures under stress. While...

journal_title：Cellular and molecular bioengineering

authors： Liang X,Graf BW,Boppart SA

更新日期：2011-06-01 00:00:00

abstract：:Normal cardiomyocytes are highly dependent on the functional expression of ion channels to form action potentials and electrical coupling with other cells. To fully determine the scientific and therapeutic potential of stem cells for cardiovascular-disease treatment, it is necessary to assess comprehensively the regul...

journal_title：Cellular and molecular bioengineering

authors： Ma Z,Liu Q,Liu H,Yang H,Yun JX,Xu M,Eisenberg CA,Borg TK,Markwald R,Gao BZ

更新日期：2012-09-01 00:00:00

abstract：Introduction:Microbes aggregate when they display adhesive proteins on their outer membrane surfaces, which then form bridges between microbes. Aggregation protects the inner microbes from harsh environmental conditions such as high concentrations of antibiotics, high salt conditions, and fluctuations in pH. The protec...

journal_title：Cellular and molecular bioengineering

authors： Lewis DD,Vanella R,Vo C,Rose L,Nash M,Tan C

更新日期：2018-09-06 00:00:00

abstract：:Recent evidence suggests that the spontaneously hypertensive rat (SHR) has an elevated level of proteases, including matrix metalloproteinases (MMPs), involved in cell membrane receptor cleavage. We hypothesize that SHR red blood cells (RBCs) may be subject to an enhanced glycocalyx cleavage compared to the RBCs of th...

journal_title：Cellular and molecular bioengineering

authors： Pot C,Chen AY,Ha JN,Schmid-Schönbein GW

更新日期：2011-12-01 00:00:00

abstract：:Ventilator-induced lung overdistension has been a growing concern in the management of mechanically ventilated patients. Mechanical ventilation triggers or enhances the net inflammatory and tissue remodeling activities. Although it has been shown that proinflammatory and tissue remodeling factors play important roles ...

journal_title：Cellular and molecular bioengineering

authors： Patel H,Kwon S

更新日期：2009-12-01 00:00:00

abstract：:Endometriosis is a chronic disease in which epithelial and stromal cells that resemble the eutopic endometrium are found in ectopic lesions. In order to examine how microenvironmental factors such as extracellular matrix and macrophages influence disease progression, 12Z (an immortalized ectopic epithelial cell line) ...

journal_title：Cellular and molecular bioengineering

authors： Pollock K,Jaraczewski TJ,Carroll MJ,Lebovic DI,Kreeger PK

更新日期：2014-09-01 00:00:00

abstract：:Primary cilia are sensory organelles that have been shown to play a critical role in lineage commitment. It was our hypothesis that the primary cilium is necessary for chemically induced differentiation of human mesenchymal stem cells (MSC). To investigate this, polaris siRNA was used to inhibit the primary cilia and ...

journal_title：Cellular and molecular bioengineering

authors： Tummala P,Arnsdorf EJ,Jacobs CR

更新日期：2010-09-01 00:00:00

abstract：INTRODUCTION:Intracellular delivery is a key step for many applications in medicine and for investigations into cellular function. This is particularly true for immunotherapy, which often requires controlled delivery of antigen and adjuvants to the cytoplasm of immune cells. Due to the complex responses generated by th...

journal_title：Cellular and molecular bioengineering

authors： Du F,Liu YG,Scott EA

更新日期：2017-10-01 00:00:00

abstract：:The stromal vascular fraction (SVF) of human adipose tissue is a heterogeneous population, with component cell types that may or may not contribute to its regenerative potential. Recent findings indicate that single-cell mechanical biomarkers are characteristic of cell type and can be used comparably to gene and prote...

journal_title：Cellular and molecular bioengineering

authors： Kanthilal M,Darling EM

更新日期：2014-12-01 00:00:00

abstract：:The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell-cell communication, a protein consisting of the extracellular recognition domains of E-cadherin was associated with the surface of silica beads by either tethering to a ...

journal_title：Cellular and molecular bioengineering

authors： Tsai J,Kam LC

更新日期：2010-03-01 00:00:00

abstract：:We used principal component analysis to dissect the mechanics of chemotaxis of amoeboid cells into a reduced set of dominant components of cellular traction forces and shape changes. The dominant traction force component in wild-type cells accounted for ~40% of the mechanical work performed by these cells, and consist...

journal_title：Cellular and molecular bioengineering

authors： Alonso-Latorre B,Del Álamo JC,Meili R,Firtel RA,Lasheras JC

更新日期：2011-12-01 00:00:00