Abstract：
:The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and - to a lower extent - to XBSM may influence osteoblast activity in vivo.
journal_name
J Biomater Appljournal_title
Journal of biomaterials applicationsauthors
Kyyak S,Blatt S,Pabst A,Thiem D,Al-Nawas B,Kämmerer PWdoi
10.1177/0885328220914407subject
Has Abstractpub_date
2020-07-01 00:00:00pages
83-96issue
1eissn
0885-3282issn
1530-8022journal_volume
35pub_type
杂志文章abstract：:Using a hydraulic stress simulator, the mechanical behavior of the porcine pericardium used in the construction of cardiac valve leaflets was characterized following the same procedure employed with calf pericardium in Part 1 of this study. One hundred fifty pairs of tissue samples were subjected to tensile testing to...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
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更新日期：2001-07-01 00:00:00
abstract：:To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a novel biomaterial, composite microspheres consisting of carboxymethyl chitosan, sodium alginate, and collagen (CSCM). The ultra-structure of CSCM was investigated by scanning electron microscopy assay. In hemostatic fu...
journal_title：Journal of biomaterials applications
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abstract：:Nonviral gene delivery systems are finding widespread use due to their safety, rapid and economical production, and ease of modification. In this work, series of N-alkyl-substituted linear polyethylenimine (CP) polymers have been synthesized, characterized, and investigated about how degree of substitution (hydrophobi...
journal_title：Journal of biomaterials applications
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abstract：:Injectable thermo-sensitive hydrogels have a potential application in bone tissue engineering for their sensitivities and minimal invasive properties. Human dental pulp stem cells have been considered a promising tool for tissue reconstruction. The objective of this study was to investigate the proliferation and osteo...
journal_title：Journal of biomaterials applications
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更新日期：2016-09-01 00:00:00
abstract：:The paper studies the coupling reaction by covalent bonding of acetylamino-2-sulfamoyl-1,3,4-thiodiazole (AcAA) on the poly(acrylic acid-costyrene) copolymer (PAcA-S) in homogeneous system, in the presence of dicyclohexylcarbodiimide (DCC) as activator. The influence of some factors on coupling efficiency (the drug/su...
journal_title：Journal of biomaterials applications
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abstract：:The surface quality of coronary stents has a significant influence on its biocompatibility. Therefore, surface polishing is of paramount importance in the production and application of stents. In the present study, electropolishing is performed on 316LVM steel slotted tube coronary stents. Additionally, acid pickling,...
journal_title：Journal of biomaterials applications
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abstract：:Biodegradable sponges, as a promising hemostatic biomaterial, has been clinically required over the past decades. Current hemostatic sponges are generally prepared by crosslinking and freeze-drying, but the quality control or biocompatibility is often unsatisfactory due to the freezing-caused morphological non-uniform...
journal_title：Journal of biomaterials applications
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更新日期：2017-03-01 00:00:00
abstract：:Sternotomy and sternal closure occur prior to and post cardiac surgery, respectively. Although post-operative complications associated with poor sternal fixation can result in morbidity, mortality, and considerable resource utilization, sternotomy is preferred over other methods such as lateral thoracotomy. Rigid ster...
journal_title：Journal of biomaterials applications
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更新日期：2013-11-01 00:00:00
abstract：:Sodium alginate is an effective biomaterial for tissue engineering applications. Non-purified alginate is contaminated with protein, lipopolysaccharide, DNA, and RNA, which could elicit adverse immunological reactions. We developed a purification protocol to generate biocompatible alginate based on (a) activated charc...
journal_title：Journal of biomaterials applications
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更新日期：2016-04-01 00:00:00
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journal_title：Journal of biomaterials applications
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更新日期：2013-08-01 00:00:00
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journal_title：Journal of biomaterials applications
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doi：10.1106/5JUJ-TFJ3-JVVA-3RJ0
更新日期：2001-01-01 00:00:00
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journal_title：Journal of biomaterials applications
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doi：10.1177/0885328220930714
更新日期：2020-10-01 00:00:00
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journal_title：Journal of biomaterials applications
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doi：10.1177/0885328209344003
更新日期：2010-08-01 00:00:00
abstract：:Poly(2-hydroxyethyl methacrylate) hydrogel intraocular lenses, containing adrenochrome-melanin, were manufactured and implanted in animal eyes in order to assess the effect of melanin upon (a) biocompatibility of implants with the eye tissues, and (b) fibrous proliferation of lens epithelium responsible for the opacif...
journal_title：Journal of biomaterials applications
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doi：10.1177/088532829500900305
更新日期：1995-01-01 00:00:00
abstract：:The synthesis, tensile strength, thermostability and hydrophilicity of the poly(urethaneamideimide) and the poly(urethaneureas) with N-aliphatic side chains are presented. These elastomers were compared in blood compatibility with determination of the common indices and with immunologic tests of the beta-thromboglobul...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1106/TBUW-TWW9-EC7X-YA3H
更新日期：2000-10-01 00:00:00
abstract：:The layer-by-layer technique, which allows simple preparation of polyelectrolyte multilayers, came into the focus of research for development of functionalized medical devices. Numerous literature exist that concentrate on the film build-up and the behaviour of cells on polyelectrolyte multilayers. However, in case of...
journal_title：Journal of biomaterials applications
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更新日期：2013-07-01 00:00:00
abstract：:The aim of this study was to examine the release of silica (Si), calcium (Ca), phosphorous (P), and fluoride (F) from conventional glass ionomer cement (GI) and resin-modified glass ionomer cement (LCGI), containing different quantities of bioactive glass (BAG). Further aim was to evaluate in vitro biomineralization o...
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更新日期：2004-07-01 00:00:00
abstract：:Bioimaging at a subcellular resolution to label cytoplasm and nucleus in living cell by just one photoluminescent nanoparticle has great application potential in bioresearch, preclinical diagnosis, screening, and image-guided therapy of life-threatening diseases. Herein, we report a novel arginine (Arg) functionalized...
journal_title：Journal of biomaterials applications
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abstract：:The aim of this pilot study was to evaluate the bioactive, surface-coated polycaprolactone-co-lactide scaffolds as bone implants in a tibia critical size defect model. Polycaprolactone-co-lactide scaffolds were coated with collagen type I and chondroitin sulfate and 30 piled up polycaprolactone-co-lactide scaffolds we...
journal_title：Journal of biomaterials applications
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更新日期：2012-01-01 00:00:00
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journal_title：Journal of biomaterials applications
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更新日期：2008-11-01 00:00:00
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journal_title：Journal of biomaterials applications
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abstract：:Gold nanomaterials possess unique physical and chemical properties, which attracted much attention in recent years. As a new type of gold nanomaterials, gold nanostars (GNSTs) have been prepared and characterized in this study. GNSTs under near-infrared (NIR) light irradiation can exert not only cancer photothermal th...
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abstract：:Melt electrowriting (MEW) can print high-resolution scaffolds with the ultrafine fibers from 800 nm to 20 µm. However, the cell seeding efficiency relatively low due to the large pore size of the MEW scaffold. Here, we reported a method to solve this dilemma by electrospinning a gelatin methacrylate (GelMA) hydrogel f...
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abstract：:A range of crystalline and nano-sized carbonate- and silicate-substituted hydroxyapatite has been successfully produced by using continuous hydrothermal flow synthesis technology. Ion-substituted calcium phosphates are better candidates for bone replacement applications (due to improved bioactivity) as compared to pha...
journal_title：Journal of biomaterials applications
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abstract：:Hydroxyapatite (Ca10(PO4)6(OH)2) is widely investigated as an implantable material for hard tissue restoration due to its osteoconductive properties. However, hydroxyapatite in bulk form is limited as its mechanical properties are insufficient for load-bearing orthopedic applications. Attempts have been made to improv...
journal_title：Journal of biomaterials applications
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