Abstract：
OBJECTIVE:This study aims to investigate the roles of miR-221-3p and miR-222-3p in the regulation of osteogenic differentiation of bone marrow mesenchyme stem cells (BMSCs) under high glucose condition. MATERIALS AND METHODS:The expreesions of miR-221-3p, miR-222-3p and insulin-like growth factor 1 (IGF-1) were detected by qRT-PCR. The protein levels of osteoblast-related proteins (Osterix, Runx-2 and Osteopontin) were detected by western blot. Whether miR-221-3p and miR-222-3p can target IGF-1 was assessed by dual luciferase reporter gene assay. RESULTS:miR-221-3p and miR-222-3p were up-regulated in the mandibles of diabetic rats and BMSCs cultured in high glucose condition. Silencing miR-221-3p or/ and miR-222-3p increased ALP activity and up-regulated osteoblast-related protein levels, and the simultaneous silence the two miRNAs showed stronger effects on ALP activity and osteoblast-related protein levels. Next, we confirmed that miR-221-3p and miR-222-3p both targeted IGF-1 and cooperatively regulated its expression. Besides, miR-221-3p and miR-222-3p regulated ERK activation through IGF-1. Silencing miR-221-3p and miR-222-3p promoted osteogenic differentiation of BMSCs through IGF-1 under high glucose condition. CONCLUSION:miR-221-3p and miR-222-3p inhibited osteogenic differentiation of BMSCs via IGF-1/ERK pathway under high glucose condition.
journal_name
Diabetes Res Clin Practjournal_title
Diabetes research and clinical practiceauthors
Gan K,Dong GH,Wang N,Zhu JFdoi
10.1016/j.diabres.2020.108121subject
Has Abstractpub_date
2020-09-01 00:00:00pages
108121eissn
0168-8227issn
1872-8227pii
S0168-8227(20)30371-5journal_volume
167pub_type
杂志文章abstract：:We examined the effect of aldehyde-modified matrix macromolecules on mesangial cell function in vitro, using incubated rat glomerular mesangial cells. Laminin and fibronectin were modified by incubation for 24 h with 50 mM glycolaldehyde (GA), a highly reactive cross-linking glycation product, with or without equimola...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(94)90123-6
更新日期：1994-02-01 00:00:00
abstract：AIMS:Black Africans are disproportionally affected by type 2 diabetes, but the pathophysiology is poorly understood. The study aimed to examine the effect of sex and age on insulin sensitivity and insulin response in black South African adults. METHODS:This cross-sectional study included a random sample of 179 men and...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2016.11.005
更新日期：2016-12-01 00:00:00
abstract：AIMS:Diabetic patients require care from a variety of health care providers, but little is known about their actual use of health care. Aims of this study were to (1) obtain information on health care use by diabetic patients in the Netherlands, (2) distinguish patterns of health care utilisation among these patients, ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(03)00116-5
更新日期：2003-09-01 00:00:00
abstract：:Vascular complications, as a consequence of atherosclerosis, are the main causes of morbidity and mortality in diabetes. Low density lipoprotein (LDL) oxidation is accepted as a relevant pathogenic mechanism in atherogenesis. The aim of this work was to study the relationship between lipid peroxidation (LPO) and metab...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(97)00032-6
更新日期：1997-05-01 00:00:00
abstract：AIMS:To determine whether chronic life stress is differentially associated with quality of life (QoL) for Blacks vs. Hispanics with type 2 diabetes. METHODS:We assessed self-reported chronic stress and QoL in 125 patients with type 2 diabetes who self-identified as either non-Hispanic Black or Hispanic. Separate cross...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2015.01.025
更新日期：2015-04-01 00:00:00
abstract：:Non-insulin-dependent diabetes mellitus (NIDDM) is a major clinical and public health problem in Kuwait. The objective of the study was to determine prevalence rates of NIDDM among a representative sample of the Kuwaiti adult population aged 20 and older in two out of five governorates and identify the associated risk...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(98)00104-1
更新日期：1998-12-01 00:00:00
abstract：:Metabolic syndrome affects approximately one quarter of population in developed countries. Its presence is a major risk for development of both type 2 diabetes (T2DM) and atherosclerosis. The prevalence of cardiovascular disease is 2-3 times higher in individuals with metabolic syndrome than in age-matched controls. M...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/j.diabres.2005.03.002
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:The presence of diabetes in pregnancy can result in substantial morbidity to both mother and baby if management is sub-optimal. AIMS:To assess the process of standards of preconception care (against the National Service Framework standards) of women attending the adult general diabetes clinics in a district...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2006.07.025
更新日期：2007-04-01 00:00:00
abstract：AIMS:To develop a risk "engine" or calculator, integrating the risks of hyperglycemia, maternal BMI and other basic demographic data commonly available at the time of the pregnancy oral glucose tolerance test (OGTT), to predict an individual's absolute risk of specific adverse pregnancy outcomes. METHODS:Data from the...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/j.diabres.2018.02.036
更新日期：2018-05-01 00:00:00
abstract：AIMS:There are numerous risk or screening scores for the prediction of type-2 diabetes mellitus (DM). In contrast, few scores are available for preDM. In this paper, we compare the two screening scores from the American Diabetes Association (ADA) and Centers for Disease Control and Prevention (CDC) that can be used for...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2016.06.022
更新日期：2016-08-01 00:00:00
abstract：AIM:To describe coverage, cost and care of type 1 diabetes (T1D) in 2 regions of China--Beijing and Shantou--including: METHODS:This is a mixed-methods descriptive study with three arms--coverage, cost and care. It is taking place in 4 tertiary hospitals, 3 secondary hospitals and 4 primary health facilities in Beijin...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2011.10.016
更新日期：2011-11-01 00:00:00
abstract：AIMS:The effects of travel distance and travel time to the primary diabetes care provider and waiting time in the practice on health-related quality of life (HRQoL) of patients with type 2 diabetes are investigated. RESEARCH DESIGN AND METHODS:Survey data of 1313 persons with type 2 diabetes from six regions in Englan...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2017.01.014
更新日期：2017-04-01 00:00:00
abstract：:To elucidate the mechanism responsible for the decreased insulin binding to erythrocytes in uremic patients, the effects of incubation with sera obtained from uremic patients or with methylguanidine, respectively, on insulin binding were examined. Insulin binding to erythrocytes from uremic patients was lower than tha...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(91)90061-h
更新日期：1991-09-01 00:00:00
abstract：AIMS:To investigate serum levels of thyroid stimulating hormone (TSH), anti-thyroid peroxidase antibody (TPO), and 25(OH)D in the presence or absence of metabolic syndrome in an obese population. METHODS:Data from a prospectively generated "Obesity Polyclinic" database that includes socio-demographic characteristics, ...
journal_title：Diabetes research and clinical practice
pub_type： 临床试验,杂志文章
doi：10.1016/j.diabres.2014.08.001
更新日期：2014-10-01 00:00:00
abstract：:The exocrine and endocrine pathophysiology of chronic calcific pancreatitis of the tropics (CCPT) remains elusive. The objective of this study was to evaluate the spectrum and correlates of the exocrine and endocrine pancreatic dysfunction in CCPT. Thirty-seven consecutive patients with a clinico-radiological diagnosi...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(94)01021-q
更新日期：1995-02-01 00:00:00
abstract：AIM:To summarize the existing literature concerning the association between polypharmacy and adverse health consequences in elderly patients with type 2 diabetes mellitus. METHODS:We searched four literature databases (PubMed/Medline, ScienceDirect and Web of Science) through April 2019. We included all studies that a...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,meta分析
doi：10.1016/j.diabres.2019.107804
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:To investigate factors related to diabetic retinopathy (DR). METHOD:Clinical data of 603 Chinese diabetic patients were analyzed retrospectively. On the basis of its severity, DR was graded as non-diabetic retinopathy (NDR), non proliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2011.02.007
更新日期：2011-05-01 00:00:00
abstract：AIMS:Diabetes regimen distress (RD) and depression are related constructs, however the nature of their relationship has not been explored over time, leading to difficulties differentiating between RD and depression and for selection of programs of care. We examined longitudinal associations between RD and depression to...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.diabres.2014.12.017
更新日期：2015-05-01 00:00:00
abstract：AIMS:We investigated the 2-year clinical outcomes of patients with acute myocardial infarction (AMI) and prediabetes after new-generation drug-eluting stents implantation. METHODS:A total of 11,962 patients with AMI were classified into normoglycemia (group A; 3,080), prediabetes (group B; 3,709), and diabetes (group ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2019.107994
更新日期：2020-02-01 00:00:00
abstract：AIMS:To evaluate the impact of gestational diabetes mellitus (GDM) and maternal height on offspring birthweight. METHODS:This is an observational cohort study, encompassing 4 111 Finnish primiparous women from Vantaa city, Finland, with singleton deliveries between 2009 and 2015. Data were obtained from the Finnish Me...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2019.01.004
更新日期：2019-02-01 00:00:00
abstract：:The aim of this study was to compare the effect of nasally administered glucagon in doses of 1 (A) and 2 mg (B), with 1 mg glucagon administered intramuscularly (C) in 12 C-peptide-negative IDDM patients. Spontaneous recovery (D) from insulin-induced hypoglycaemia in the same patients was used as reference. The mean a...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(92)90042-p
更新日期：1992-07-01 00:00:00
abstract：:Cause(s) of death in patients with diabetes mellitus (DM) admitted to a tertiary care hospital in North India was analysed from classified information in patients' death records and case file. Of the 306652 total admissions from 1991 to 1999, 21584 patients died (7.04%), 592 of whom (355 men and 237 women) had DM (2.7...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(03)00013-5
更新日期：2003-05-01 00:00:00
abstract：:Glucose metabolism disturbances are frequently reported among patients with beta-thalassaemia major on conventional treatment consisted of regular blood transfusions and adequate chelation treatment. Aim of this study was to evaluate the evolution of oral glucose tolerance test (OGTT) in thalassaemic patients in relat...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2006.07.010
更新日期：2007-04-01 00:00:00
abstract：:Expansion of the glomerular mesangium is a consistent finding of diabetic nephropathy. Negatively charged proteoglycans are an integral part of the mesangium and their synthesis and degradation is disturbed in many forms of glomerulosclerosis. The metabolism of ascorbic acid (AA), which plays an important role in extr...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(97)00063-6
更新日期：1997-08-01 00:00:00
abstract：AIMS:Diabetes therapies that provide durable glycaemic control for people with type 2 diabetes mellitus (T2DM) are needed. We present efficacy results of albiglutide, a glucagon-like peptide-1 receptor agonist, in people with T2DM over a 3-year period. METHODS:Five of the 8 HARMONY phase 3 trials, comparing albiglutid...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.diabres.2017.06.013
更新日期：2017-09-01 00:00:00
abstract：:This random multistage cross-sectional population survey was undertaken to determine the prevalence of diabetes mellitus (DM) and impaired glucose tolerance (IGT) in subjects aged 25 years and above in India. The study was carried out in 77 centers (40 urban and 37 rural). 18363 (9008 males and 9355 females) subjects ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2004.04.008
更新日期：2004-12-01 00:00:00
abstract：AIMS:Centrally administered insulin improves cognitive functions in patients with Alzheimer's disease; however, it remains unknown whether long-acting insulin analogs exert more pronounced effects than insulin. In the present study, we directly compared the effects of insulin and its analogs on neural functions in vitr...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2017.03.032
更新日期：2017-07-01 00:00:00
abstract：AIMS:We tested the hypothesis that short sleep duration is not only a risk factor for diabetes, but that the two conditions in combination would be associated with the risk of incident cardiovascular disease (CVD). METHODS:We analyzed 1255 hypertensive patients (mean age: 70.4±9.9 years) with (N=299) and without diabe...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.09.014
更新日期：2012-12-01 00:00:00
abstract：AIMS:To explore epidemiological trends in type 2 diabetes mellitus (T2D) in the US between 2007 and 2012 using a large US claims database, with a particular focus on demographics, prevalence, newly-diagnosed cases, and comorbidities. METHODS:Truven Health MarketScan® Databases were used to identify patients with claim...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2016.04.043
更新日期：2016-07-01 00:00:00
abstract：:The impact of several environmental and genetic factors on diabetes and its complications is well documented. The present study was aimed to examine the serum levels of IL-17A and IFN-gamma as antagonistic cytokines in nephropathic and non-nephropathic type-2 diabetic patients. In this experimental study, serum sample...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2009.09.027
更新日期：2010-01-01 00:00:00
