Whole-Neuron Synaptic Mapping Reveals Spatially Precise Excitatory/Inhibitory Balance Limiting Dendritic and Somatic Spiking.

Abstract:

:The balance between excitatory and inhibitory (E and I) synapses is thought to be critical for information processing in neural circuits. However, little is known about the spatial principles of E and I synaptic organization across the entire dendritic tree of mammalian neurons. We developed a new open-source reconstruction platform for mapping the size and spatial distribution of E and I synapses received by individual genetically-labeled layer 2/3 (L2/3) cortical pyramidal neurons (PNs) in vivo. We mapped over 90,000 E and I synapses across twelve L2/3 PNs and uncovered structured organization of E and I synapses across dendritic domains as well as within individual dendritic segments. Despite significant domain-specific variation in the absolute density of E and I synapses, their ratio is strikingly balanced locally across dendritic segments. Computational modeling indicates that this spatially precise E/I balance dampens dendritic voltage fluctuations and strongly impacts neuronal firing output.

journal_name

Neuron

journal_title

Neuron

authors

Iascone DM,Li Y,Sümbül U,Doron M,Chen H,Andreu V,Goudy F,Blockus H,Abbott LF,Segev I,Peng H,Polleux F

doi

10.1016/j.neuron.2020.02.015

subject

Has Abstract

pub_date

2020-05-20 00:00:00

pages

566-578.e8

issue

4

eissn

0896-6273

issn

1097-4199

pii

S0896-6273(20)30138-0

journal_volume

106

pub_type

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