Abstract:
:We performed a comparative study of the cytotoxicity of cisplatin, JM216 complex, and aminonitroxyl platinum(IV) complexes for HeLa cells grown in monolayer and 3D culture. The growth dynamics of spheroids was studied and optimal conditions for evaluation of cytotoxicity were determined. Spheroids were less sensitive to the test compounds than cells in a monolayer. The resistance index (RI) of spheroids was determined as the ratio of IC50 for spheroids to IC50 for monolayer culture. Resistance index was 5.0±1.5 for cisplatin and ranged from 1.8 to 2.3 for platinum(IV) complexes. The observed differences are related to different physicochemical properties of the complexes and different mechanisms of their penetration into cells.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Trigub ММ,Filatova NV,Areshidze DА,Sen' VD,Terentiev ААdoi
10.1007/s10517-020-04757-1subject
Has Abstractpub_date
2020-02-01 00:00:00pages
583-588issue
4eissn
0007-4888issn
1573-8221pii
10.1007/s10517-020-04757-1journal_volume
168pub_type
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