Abstract:
BACKGROUND:At present, autologous intestinal segments are often used for bladder reconstruction. However, the gastrointestinal mucosa often causes various complications. METHODS:Oral mucosal tissues were obtained from the buccal cavity of beagle dogs. Primary oral mucosal epithelial cells were cultured on temperature-responsive culture dishes with a mitomycin C-treated 3T3 feeder layer for 2 weeks. Cultured epithelial cells were harvested as contiguous sheets by reducing the temperature to 20°C. The study consisted of three groups. In group 1, oral mucosal epithelial cell sheets were autografted on demucosalized gastric flaps. Next, the gastric flaps with the oral mucosal epithelial cell sheets were used for bladder reconstruction. Bladder reconstruction was once immediately and then 5 days after epithelial cell sheet grafting in groups 2 and 3, respectively. Three weeks after bladder reconstruction, the gastric flaps with the oral mucosal epithelial cell sheets were examined by immunohistology. RESULTS:Flaps grafted with oral mucosal epithelial cell sheets showed epithelial regeneration in groups 1 and 3. Regenerated epithelia were stratified and similar to native oral mucosa. However, the regenerated epithelium was absent from the reconstructive segment, and urothelial ingrowth was observed in group 2. Macroscopically, all reconstructive segments showed contracture. CONCLUSIONS:We successfully performed a bladder reconstruction using oral mucosal epithelial cell sheet-grafted flaps that exhibited epithelial regeneration. Further study should consider shrinkage prevention.
journal_name
Transplantationjournal_title
Transplantationauthors
Watanabe E,Yamato M,Shiroyanagi Y,Tanabe K,Okano Tdoi
10.1097/TP.0b013e31820e0170subject
Has Abstractpub_date
2011-04-15 00:00:00pages
700-6issue
7eissn
0041-1337issn
1534-6080journal_volume
91pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::Preformed recipient HLA-specific antibodies can cause hyperacute rejection of a transplanted kidney if they are directed against mismatched donor HLA antigens. To avoid hyperacute rejection it is essential that recipient antibodies be identified during patient workup for transplantation and HLA antigens to which a pat...
journal_title:Transplantation
pub_type: 评论,杂志文章
doi:10.1097/00007890-200105270-00002
更新日期:2001-05-27 00:00:00
abstract:BACKGROUND:Cardiac allograft vasculopathy (CAV) is a major limitation in long-term graft survival after heart transplantation (HTx). Its prediction and detection at an early stage is a challenge because an accurate, minimally invasive blood test is lacking. The aim of this study was to analyze the relationship of Tact ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000493
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:The novel agent sirolimus (SRL; Rapamune; rapamycin) inhibits the immune response by a mechanism distinct from those of calcineurin antagonists or antimetabolites. This randomized, controlled, multicenter, single blind, phase II trial examined the combination of SRL, steroids, and full versus reduced doses o...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00007890-199911270-00016
更新日期:1999-11-27 00:00:00
abstract:BACKGROUND:Airway complications continue to be an important source of morbidity and mortality after lung transplantation (LTx). Different approaches have been used for their nonsurgical management. We describe our experience using self-expandable metallic stents (SEMSs) in patients with airway complications post-LTx. ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000061229.83500.A0
更新日期:2003-05-15 00:00:00
abstract:BACKGROUND:Blood group-incompatible transplantation is one strategy used when a potential recipient does not have a compatible living donor. Current practice includes desensitization strategies to reduce antibody titers. However, when antibodies are low, in cardiac transplantation in neonates for example, no desensitiz...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825b7608
更新日期:2012-08-27 00:00:00
abstract::The NOD mouse develops immune-mediated diabetes mellitus characterized by T cell infiltration and destruction of pancreatic islet tissue. We wished to determine whether one contributing factor was an abnormality of the NOD pancreas that caused it to elicit an attack by NOD T cells. Therefore we constructed mice that h...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199206000-00032
更新日期:1992-06-01 00:00:00
abstract:BACKGROUND:A recent clinical study demonstrated that in renal allografts preserved in the cold apoptosis occurred soon after reperfusion. The mode of cell death during cold storage is generally considered necrotic. Whether apoptosis occurs as a part of cold storage is uncertain. The objective was to determine in human ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200109150-00010
更新日期:2001-09-15 00:00:00
abstract:BACKGROUND:Organs for transplantation are a scarce community resource but community preferences and how they are incorporated into allocation policies are unclear. This systematic review aimed to ascertain community preferences for organ allocation and the principles underpinning these preferences. METHODS:Medline, Em...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e3181cf1ee1
更新日期:2010-04-15 00:00:00
abstract::Natural xenoantibodies are believed to be IgM in nature and are known to play a critical role in the hyperacute rejection of distantly related xenografts. The purpose of this study was to determine whether the reducing agent DL-penicillamine could inactivate baboon natural xenoantibodies to pig splenocytes. Pooled bab...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-12-27 00:00:00
abstract::This is a short review of tolerance from the point of view of the clinician. Various examples of tolerance occurring in patients and animal models that relate to the clinical experience are described. There may be different mechanisms by which tolerance is achieved, but from the patient's point of view operational tol...
journal_title:Transplantation
pub_type: 历史文章,杂志文章
doi:10.1097/01.tp.0000117776.14277.03
更新日期:2004-03-27 00:00:00
abstract::A patient with end-stage renal disease and known benign monoclonal gammopathy underwent kidney transplantation at Westchester County Medical Center, Valhalla, NY. After surgery, during routine follow-up, the patient had laboratory evidence of frank multiple myeloma. However, she did not show any clinical signs or symp...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-199612150-00008
更新日期:1996-12-15 00:00:00
abstract::The purpose of this study was to investigate the effect of prostaglandin E1 on rabbit heart preservation. The hearts were arrested with cardioplegia (CP) solution, followed by intracoronary infusion of Euro-Collins (E-C) solution. They were immersed in E-C solution at 4 degrees C for 9 hr, flushed with terminal warm C...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199209000-00006
更新日期:1992-09-01 00:00:00
abstract::The effect of FK506 and cyclosporine on NK and ADCC activity was evaluated. Cytotoxic cells mediating both activities are resistant to FK506. In contrast, preincubation of ADCC and NK effectors with cyclosporine decreases their cytotoxic potential, while adding the drug to the assays is without effect. ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199101000-00045
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Ex situ normothermic machine perfusion (NMP) can be used to assess viability of suboptimal donor livers before implantation. Our aim was to assess the diagnostic accuracy of bile biochemistry for the assessment of bile duct injury (BDI). METHODS:In a preclinical study, 23 human donor livers underwent 6 hour...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002500
更新日期:2019-07-01 00:00:00
abstract::The beneficial effect of deoxyspergualin (DSG, NKT-01) on lethal graft-versus-host disease in mice has been studied in a major histoincompatible donor-recipient combination. Suppression of the effector mechanisms responsible for the lethal outcome in this GVHD model is also noted. This study reveals: (1) DSG has a mar...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199103000-00032
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:Recipients with multiple (more than one) acute rejection (AR) episodes have significantly lower graft survival rates than those with no AR or only one treated episode. However, fewer than 50% of recipients treated for one AR episode will have another episode. METHODS:We studied recipients with at least one ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200006150-00024
更新日期:2000-06-15 00:00:00
abstract:BACKGROUND:Hemostatic complications are not uncommon after bone marrow transplantation (BMT). However, little is known about the frequency, localization, determinants, and outcome of hemostatic events in autologous and allogeneic BMT. METHODS:Four hundred forty-seven patients (364 allogeneic, 83 autologous transplants...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200211150-00018
更新日期:2002-11-15 00:00:00
abstract:BACKGROUND:The impact of the age of the donor on the outcome of living related liver transplantation is yet to be clarified. METHODS:During October 14, 1996 and December 20, 1999, 34 living related liver transplantations were performed. Of these, 26 cases were performed using the extended left lobe graft, which were c...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200012270-00007
更新日期:2000-12-27 00:00:00
abstract:BACKGROUND:Vitamin D deficiency has been reported in different chronic pulmonary diseases like asthma and chronic obstructive pulmonary disease, but little is known in lung transplant recipients. METHODS:Serum 25-hydroxyvitamin D (25-OHD) levels and pulmonary function (forced expiratory volume in 1 sec [FEV(1)] %predi...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31823d98bc
更新日期:2012-01-27 00:00:00
abstract:BACKGROUND:In the hamster to rat xenogeneic combination, antibodies, T cells, and natural killer (NK) cells have all been implicated in the process of rejection. 3.2.3 is a mouse IgG1kappa monoclonal antibody (mAb) directed against NKR-P1A on rat NK cells. The purpose of this study was to evaluate the effect of this mA...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199803150-00021
更新日期:1998-03-15 00:00:00
abstract:BACKGROUND:Pancreas transplantation (PTX) improves lipids in patients with type 1 diabetes mellitus. However, there are patients who have persistent abnormal lipids or develop new hyperlipidemia despite PTX. One factor that may influence the lipid profile is apolipoprotein E (Apo E) genotype. Apo E polymorphism, partic...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200210150-00013
更新日期:2002-10-15 00:00:00
abstract:BACKGROUND:The use of liver allografts from an older donor (OD) (age>50 years) is a widespread strategy to manage the disparity between supply and demand of organs for liver transplantation. This study determines the effect of OD allografts on fibrosis progression and graft survival after liver transplantation in patie...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000095896.07048.BB
更新日期:2004-01-15 00:00:00
abstract::The immunosuppressive activity of niridazole in unmatched kidney allografts in nephrectomized dogs was studied. The drug alone was not effective, but improved survival when used with azathioprine and prednisolone or azathioprine alone. However, niridazole was toxic in dogs in the doses used (25 or 50 mg/kg/day). ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198004000-00009
更新日期:1980-04-01 00:00:00
abstract::Antibodies against B lymphocytes were found in the serum of the majority of 59 kidney transplant recipients and of 22 eluates obtained from kidney allografts undergoing rejection. To characterize these B cell lymphocytotoxins we have used a mouse monoclonal anti-DR antibody (L227) that inhibits cytotoxicity of antibod...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198305000-00011
更新日期:1983-05-01 00:00:00
abstract::In vitro pretreatment of islets of Langerhans with deoxyguanosine (dGuo) has been shown to be effective for the prolongation of islet allograft survival in rats. [This study evaluates the effect of pretreatment of islets with dGuo transplanted into CsA-treated recipients.] Transplantation of dGuo-treated islets from W...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199105000-00007
更新日期:1991-05-01 00:00:00
abstract::In a prospective longitudinal study, detection of cytomegalovirus (CMV) DNA in plasma (plasma polymerase chain reaction [PCR]) was compared with PCR of CMV DNA in peripheral blood leukocytes (PBL PCR), the CMV pp65 antigenemia assay, and viral cultures from blood, urine, and throat of 29 patients, 14 of whom received ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199707150-00020
更新日期:1997-07-15 00:00:00
abstract:BACKGROUND:The objective of this study was to investigate purine nucleotide metabolism in peripheral blood mononuclear cells (PBMC) of cardiac transplant recipients switched from azathioprine to mycophenolate mofetil (MMF). METHODS:Concentrations of guanosine 5'triphosphate (GTP) and adenosine 5'triphosphate (ATP), th...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/01.tp.0000225759.54343.23
更新日期:2006-07-15 00:00:00
abstract:BACKGROUND:Steroid-resistant acute rejection is a risk factor for inferior renal allograft outcome. METHODS:From 873 kidney transplant recipients (1995-2005), 108 patients with a first rejection episode were selected for study using strict inclusion criteria and clinical endpoint definition. We aimed to predict respon...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825db651
更新日期:2012-09-27 00:00:00
abstract:BACKGROUND:We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors. METHODS:Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000263991.74052.46
更新日期:2007-06-27 00:00:00
abstract:OBJECTIVES:To retrospectively compare the accuracy of pretransplant panel of reactivity antibodies (PRA) and serum level of soluble CD30 (sCD30) in predicting early (<6 months) acute rejection (AR) in living-donor and deceased-donor kidney-transplant (KT) patients. METHODS:Pretransplant sera of 24 KT recipients were r...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2005-05-15 00:00:00