Abstract:
OBJECTIVE:To investigate whether 25-hydroxyvitamin D [25(OH)D] can mediate effects without being converted to 1α,25-dihydroxyvitamin D [1,25(OH)2D]. METHODS:Vitamin D3 (VD3) was injected intramuscularly to 25-hydroxyvitamin D-1α-hydroxylase [1α(OH)ase] gene knockout (KO) male mice with a dose of 10,000 IU per week for 4 weeks. Skeleton Parameters and Serum biochemistry in mice were assayed. RESULTS:Serum 25(OH)D3 levels increased from 41 to 212 ng/mL in KO mice injected with VD3. Our results show that VD3 injections significantly increased the body weight of KO mice and there were no significant differences in body weight at 7 weeks of age between VD3-treated KO mice and wildtype (WT) mice. After 1 month injection, serum calcium and phosphorus levels of the KO mice were found indistinguishable from those of their WT littermates. Serum parathyroid hormone level declined significantly, but remained higher in treated KO mice. The dry weight, percentage ash weight, and calcium content of femur were returned to normal levels in VD3-treated KO mice whereas the femoral length, although increased significantly, remained significantly smaller than that of WT mice. VD3 injections also normalized the growth plate of KO mice within normal width. CONCLUSIONS:Our results demonstrate that high-dose VD3 injections can partially rescue the phenotype in 1α-hydroxylase gene KO mice. 25-Hydroxyvitamin D can mediate effects in the absence of conversion to 1α,25-dihydroxyvitamin D was confirmed in this study.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Zhang ZL,Ding XF,Tong J,Li BYdoi
10.3109/07435800.2010.542415subject
Has Abstractpub_date
2011-01-01 00:00:00pages
101-8issue
3eissn
0743-5800issn
1532-4206journal_volume
36pub_type
杂志文章abstract::This study was performed on 18 postmenopausal female volunteers in order to examine changes in sex hormone binding globulin (SHBG), high density lipoprotein cholesterol (HDLC), total cholesterol (TC) and serum triglyceride (TG) levels in a period of four months of moderate physical exercise. While SHBG decreased signi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809209111036
更新日期:1992-01-01 00:00:00
abstract::The MA-10 mouse Leydig tumor cell line produces large amounts of steroids only in response to hormonal stimulation while the R2C rat Leydig tumor cell line is constitutively steroidogenic in nature. In an effort to uncover the potential reasons for constitutive steroidogenesis in R2C cells, we have recently shown that...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016813
更新日期:2002-11-01 00:00:00
abstract:PURPOSE:Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine that plays a role in metabolic and inflammatory processes. Increasing evidence suggests that there is a link between MIF and ovulation. We aimed to evaluate plasma MIF levels in women with polycystic ovary syndrome (PCOS) and to determin...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1135442
更新日期:2016-08-01 00:00:00
abstract::Four hundred micrograms of synthetic thyrotropin releasing hormone (TRH) were given intravenously to 4 normal men and 4 normal women, and four weeks later, 1000 micrograms of TRH were administered intravenously to 4 of the 8 individuals and oxytocin (OT) was measured in plasma on both occasions. Following injection of...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808509035426
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:To determine whether daily oral micronized progesterone affects bone turnover, as estimated by serum and urine biochemical markers, in postmenopausal women on long-term estrogen replacement therapy (ERT). METHODS:We recruited 14 women aged 65 or older to participate in a 9-week trial with micronized progestero...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-120022294
更新日期:2003-05-01 00:00:00
abstract::TRH is negatively regulated by T3 both in the hypothalamic paraventricular nucleus and transient transfection models. Mutations in hTR beta 1 genes are associated with the syndrome of generalized resistance to thyroid hormone. To investigate potential effects of mutant TRs on T3 regulation of the hTRH gene, transient ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031859
更新日期:1997-11-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00
abstract::In a previous study we demonstrated a significant increase of CD5+ B subset in patients with Graves' disease (GD) compared with normal controls. The aim of this study was to compare the percentage of CD5+ B and CD5- B cells in GD with that in different forms of autoimmune and non immune-mediated thyroid diseases. Seve...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809709031844
更新日期:1997-02-01 00:00:00
abstract::The rat adrenal cortex possesses at least two mechanisms for uptake of extracellular lipoprotein cholesterol; one is receptor mediated endocytosis and the second is a non-endocytotic pathway. The latter was revealed by the ability of lipoproteins such as HDL which do not possess apo B or apo E to enhance steroid hormo...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435808409036509
更新日期:1984-01-01 00:00:00
abstract::The adrenal cortex is a dynamic organ in which the cells of the outer cortex continually divide. It is well known that this cellular proliferation is dependent on constant stimulation from peptides derived from the ACTH precursor proopiomelanocortin (POMC) since disruption of pituitary corticotroph function results in...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120016801
更新日期:2002-11-01 00:00:00
abstract::We have previously reported that the protein kinase C ligand 12-O-tetradecanoyphorbol-13-acetate (TPA) inhibited the angiotensin II (AII) stimulated CYP11B2 gene expression in the adrenocortical H295R cell line. Here we report that TPA increased the level of phospho-p44/42 MAPK but AII did not. The MEK1 inhibitor PD98...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044048
更新日期:2004-11-01 00:00:00
abstract::Cerebrovascular accidents are one of the life-threatening complications of diabetic ketoacidosis (DKA) in children and adolescents. Our objective was to evaluate the effect of DKA and its treatment on factors known to affect thrombotic activity (protein C; protein S; von Willebrand factor, fibrinogen; homocysteine; an...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120018678
更新日期:2003-02-01 00:00:00
abstract::The cdc2 kinase family is known to be one of the important factors for cell proliferation in both yeast and mammalian cells. By using polyclonal antibodies against PSTAIRE region of cdc2HS, we studied the amount of cdc2-related kinases in the rat thyroid cell line, FRTL-5, during the cell cycle. The immunoreactive pro...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809409030404
更新日期:1994-05-01 00:00:00
abstract::We report a novel mutant of the luteinizing hormone receptor (LHR) in a case of familial Leydig cell hypoplasia and pseudohermaphrotidism. The proband was homozygous for two missense mutations, T1121C and C1175T, causing substitutions I374T and T3921. The molecular effects of the mutations were investigated by heterol...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800500430890
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:Impairments in neuroendocrine regulation of food intake and postprandial satiety are leading causes to obesity. Ghrelin peptide is a GI hormone known to increase food intake partly through induction of growth hormone. The regulation of ghrelin production is still unknown. OBJECTIVE:The aim of the current ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.799484
更新日期:2014-01-01 00:00:00
abstract::A comparative study (n = 20) of serum levels of leutinizing hormone (LH) and testosterone (T) between male trained athletes (high-school students, 16-18 years old) living in North and South Shouna in the Jordan Valley (JV), 320-360 meters below sea level, and those living in Ramtha and Irbid, 550-650 meters above sea ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-100107165
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND:Stimulated thyroglobulin (STg) levels in patients with differentiated thyroid carcinomas (DTCs) after total thyroidectomy (TT) and before radioactive iodine (131I) ablation/therapy (RIT) are predictive of therapeutic success but can be influenced by the thyroid-stimulating hormone (TSH) level. OBJECTIVES:Th...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1173056
更新日期:2017-02-01 00:00:00
abstract::Tumor-promoting phorbol esters are believed to affect cell functions by activating a Ca+2-and lipid-dependent protein kinase (protein kinase C). 12-0-Tetradecanoyl phorbol-13-acetate (TPA) inhibits LH stimulation of progesterone (P) accumulation in rat granulosa cells. To determine the mechanisms by which TPA inhibits...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035438
更新日期:1986-01-01 00:00:00
abstract::The stimulation of the zona glomerulosa phenotype by a low sodium diet is characterised by increased expression of aldosterone synthase accompanied by increases in (pro)renin, bFGF, c-fos and c-jun gene transcription. In contrast ACTH diminishes the specific glomerulosa phenotype. The EGF related transmembrane protein...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032720
更新日期:1998-08-01 00:00:00
abstract::Neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) are both intrapancreatic neuropeptides that are known to inhibit stimulated insulin secretion. In the present study, we examined their influences on basal and stimulated glucagon and insulin secretion in the mouse. Either NPY or CGRP was injected intraven...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808709035466
更新日期:1987-01-01 00:00:00
abstract::Recent evidence supports the hypothesis that sympathetic neurons play a significant role in the regulation of adrenocortical cell proliferation and steroidogenesis. Co-cultures of rat adrenocortical and sympathetic ganglion cells have been established to study sympatho-adrenal interactions. In these studies we have co...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043786
更新日期:1996-11-01 00:00:00
abstract::The presence of vasoactive intestinal peptide and neuropeptide Y in thyroid nerves and their effects on thyroid blood flow are well known. However, the effects of these two neuropeptides on the various processes involved in thyroid hormone biosynthesis and release have not been fully explored. We have now tested these...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809209026678
更新日期:1992-01-01 00:00:00
abstract:AIM:To determine whether hyperuricemia is associated with the early phase and late phase of insulin release in apparently healthy non-obese subjects with normal glucose tolerance. MATERIALS AND METHODS:A population-based cross-sectional study was performed. Apparently healthy individuals aged 20 to 65 years were eligi...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2016.1155597
更新日期:2017-02-01 00:00:00
abstract::Purpose: Limited and contradictory data on the circulating levels of glucagon-like peptide (GLP-1) and resistin in hepatitis C virus genotype-4 (HCV-4) cirrhotic patients are present. Thus, this study aimed to evaluate their concentrations and to investigate the association between total GLP-1, resistin, and insulin r...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2019.1627551
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:The diagnosis and management of osteoporosis and osteoporotic fractures are challenging in rural and underdeveloped areas of China because medical resources are inaccessible; thus, a simple and accurate method is essential for the detection of vertebral fractures. We aimed to examine the relationship between...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800.2020.1827263
更新日期:2021-02-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate the expression of the glucose transporters GLUT1 and GLUT3 in papillary thyroid carcinomas (PTCs) and to elucidate their relationship with the BRAF V600E mutation and F-18 FDG uptake. MATERIALS AND METHODS:We retrospectively analyzed data of 52 PTC patients (41 women a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1066803
更新日期:2016-01-01 00:00:00
abstract::Total collagen and its soluble fraction as well as water content were determined in late pregnancy in the uterine cervix in a population of 36 rats treated with progesterone (n = 10), mifepristone (n = 13) and controls (n = 13). In both treated groups there were no signs of delivery on the 21st day of gestation. Water...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809509030477
更新日期:1995-08-01 00:00:00
abstract::Hashimoto's thyroiditis (HT) is one of the most common organ-specific autoimmune diseases. Increasing evidence indicates that HT may be characterized by an imbalance in the helper T cell subsets Th1 and Th2. Traditional Chinese Medicine (TCM) considers HT as a chronic exhaustion disease, leading to deficiency of qi. I...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1066800
更新日期:2016-01-01 00:00:00
abstract::Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immuno-reactive species to have ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048594
更新日期:2000-11-01 00:00:00
abstract::It was the aim of this study to test for a possible effect of the new non ergot dopamine agonist CV 205-502 on plasma growth hormone (GH) concentrations in acromegaly. 10 acromegalic patients received a single oral dose of 150 micrograms CV 205-502 after an overnight fast. As a control group 7 acromegalic patients und...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809409035856
更新日期:1994-02-01 00:00:00