A molecular MRI probe to detect treatment of cardiac apoptosis in vivo.

Abstract:

:Cell death by apoptosis is critical in myocardial diseases, and noninvasive detection of early, reversible apoptosis might be useful clinically. Exogenous Annexin-V (ANX) protein binds membrane phosphatidylserine, which is externalized in early apoptosis. A molecular MRI probe was constructed with superparamagnetic iron oxide (SPIO) conjugated to recombinant human ANX (ANX-SPIO). Apoptosis was induced with doxorubicin, a cardiotoxic cancer drug, in culture in neonatal rat ventricular myocytes, cardiac fibroblasts, and mesenchymal stem cells, and in vivo in the mouse heart. ANX-SPIO was validated using T2*-weighted 3T MRI. ANX-SPIO produced T2* signal loss, reflecting iron content, that correlated highly with independent apoptosis markers; bound with high affinity to apoptotic myocytes by competition assay (Ki 69 nM); detected apoptosis in culture much earlier than did TUNEL stain; and revealed fibroblast resistance to apoptosis. With apoptosis in vivo, ANX-SPIO produced diffuse myocardial T2* signal loss that correlated with increased iron stain and caspase activity. Treatment with an alpha-1-adrenergic agonist in vivo reversed apoptosis and eliminated the ANX-SPIO MRI signal. It is concluded that cardiac MRI of ANX-SPIO detects early, nonischemic cardiac apoptosis in culture and in vivo, and can identify reversibly injured cardiac cells in diseased hearts, when treatment is still possible.

journal_name

Magn Reson Med

authors

Dash R,Chung J,Chan T,Yamada M,Barral J,Nishimura D,Yang PC,Simpson PC

doi

10.1002/mrm.22876

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1152-62

issue

4

eissn

0740-3194

issn

1522-2594

journal_volume

66

pub_type

杂志文章
  • Complete elimination of the extracellular 23Na NMR signal in triple quantum filtered spectra of rat hearts in the presence of shift reagents.

    abstract::A method is suggested whereby the shifted extracellular triple quantum filtered 23Na signal of an isolated organ is completely eliminated. The method is based on the long relaxation time of the triple quantum coherence and on its fast evolution rate. When the carrier frequency is set on top of the intracellular sodium...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910300415

    authors: Navon G

    更新日期:1993-10-01 00:00:00

  • 4D radial coronary artery imaging within a single breath-hold: cine angiography with phase-sensitive fat suppression (CAPS).

    abstract::Coronary artery data acquisition with steady-state free precession (SSFP) is typically performed in a single frame in mid-diastole with a spectrally selective pulse to suppress epicardial fat signal. Data are acquired while the signal approaches steady state, which may lead to artifacts from the SSFP transient respons...

    journal_title:Magnetic resonance in medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/mrm.20627

    authors: Park J,Larson AC,Zhang Q,Simonetti O,Li D

    更新日期:2005-10-01 00:00:00

  • Free-breathing diffusion tensor MRI of the whole left ventricle using second-order motion compensation and multitasking respiratory motion correction.

    abstract:PURPOSE:We aimed to develop a novel free-breathing cardiac diffusion tensor MRI (DT-MRI) approach, M2-MT-MOCO, capable of whole left ventricular coverage that leverages second-order motion compensation (M2) diffusion encoding and multitasking (MT) framework to efficiently correct for respiratory motion (MOCO). METHODS...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28611

    authors: Nguyen CT,Christodoulou AG,Coll-Font J,Ma S,Xie Y,Reese TG,Mekkaoui C,Lewis GD,Bi X,Sosnovik DE,Li D

    更新日期:2020-11-30 00:00:00

  • Manganese-enhanced magnetic resonance imaging for in vivo assessment of damage and functional improvement following spinal cord injury in mice.

    abstract::In past decades, much effort has been invested in developing therapies for spinal injuries. Lack of standardization of clinical read-out measures, however, makes direct comparison of experimental therapies difficult. Damage and therapeutic effects in vivo are routinely evaluated using rather subjective behavioral test...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20888

    authors: Stieltjes B,Klussmann S,Bock M,Umathum R,Mangalathu J,Letellier E,Rittgen W,Edler L,Krammer PH,Kauczor HU,Martin-Villalba A,Essig M

    更新日期:2006-05-01 00:00:00

  • Image reconstruction algorithm for motion insensitive MR Fingerprinting (MRF): MORF.

    abstract:PURPOSE:The purpose of this study is to increase the robustness of MR fingerprinting (MRF) toward subject motion. METHODS:A novel reconstruction algorithm, MOtion insensitive MRF (MORF), was developed, which uses an iterative reconstruction based retrospective motion correction approach. Each iteration loops through t...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27227

    authors: Mehta BB,Ma D,Pierre EY,Jiang Y,Coppo S,Griswold MA

    更新日期:2018-12-01 00:00:00

  • Transceive surface coil array for magnetic resonance imaging of the human brain at 4 T.

    abstract::As the static magnetic field strength used in human magnetic resonance imaging increases, the wavelength of the corresponding radiofrequency field becomes comparable to the dimensions of the coil and volume of interest. The dielectric resonance effects that arise in this full wavelength regime may be partially compens...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20583

    authors: Pinkerton RG,Barberi EA,Menon RS

    更新日期:2005-08-01 00:00:00

  • MR microimaging of articular cartilage and contrast enhancement by manganese ions.

    abstract::Saline solutions of manganese ions (Mn2+) were used as articular contrast agents in magnetic resonance microscopy (9.4 T) of cartilage of chicken femoral condyles and pig temporomandibular joints. The diffusion of Mn2+ from the articular surface into the cartilage matrix led to a strong contrast enhancement in the car...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910240114

    authors: Kusaka Y,Gründer W,Rumpel H,Dannhauer KH,Gersonde K

    更新日期:1992-03-01 00:00:00

  • Template-based field map prediction for rapid whole brain B0 shimming.

    abstract:PURPOSE:In typical MRI protocols, time is spent acquiring a field map to calculate the shim settings for best image quality. We propose a fast template-based field map prediction method that yields near-optimal shims without measuring the field. METHODS:The template-based prediction method uses prior knowledge of the ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27020

    authors: Shi Y,Vannesjo SJ,Miller KL,Clare S

    更新日期:2018-07-01 00:00:00

  • Analysis and correction of biases in cross-relaxation MRI due to biexponential longitudinal relaxation.

    abstract:PURPOSE:Cross-relaxation imaging (CRI) is a family of quantitative magnetization transfer techniques that utilize images obtained with off-resonance saturation and longitudinal relaxation rate (R1) maps reconstructed by the variable flip angle (VFA) method. It was demonstrated recently that a significant bias in an app...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24677

    authors: Mossahebi P,Yarnykh VL,Samsonov A

    更新日期:2014-02-01 00:00:00

  • Transmit SENSE.

    abstract::The idea of using parallel imaging to shorten the acquisition time by the simultaneous use of multiple receive coils can be adapted for the parallel transmission of a spatially-selective multidimensional RF pulse. As in data acquisition, a multidimensional RF pulse follows a certain k-space trajectory. Shortening this...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10353

    authors: Katscher U,Börnert P,Leussler C,van den Brink JS

    更新日期:2003-01-01 00:00:00

  • Experimental encephalomyelitis modulates inositol and taurine in the spinal cord of Biozzi mice.

    abstract::In this high resolution magnetic resonance spectroscopic study of experimental allergic encephalomyelitis (EAE) and Semliki Forest virus (SFV) infection of the Biozzi AB/H mouse, marked increases in the initially low levels of N-trimethyl compounds in the spinal cord were observed during probable demyelinating episode...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910320603

    authors: Preece NE,Amor S,Baker D,Gadian DG,O'Neill JK,Urenjak J

    更新日期:1994-12-01 00:00:00

  • Water suppression with B0 field gradient homospoil pulses in high-resolution NMR spectroscopy.

    abstract::The combination of a frequency nonselective excitation suppression method (1331 sequence) with selective excitation followed by gradient-induced dephasing of water transverse magnetization yielded suppression ratios of greater than 10,000:1. The need for gradient preemphasis and correction of B0 field shifts is discus...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910090114

    authors: Brereton IM,Field J,Moxon LN,Irving MG,Doddrell DM

    更新日期:1989-01-01 00:00:00

  • Toward direct mapping of neuronal activity: MRI detection of ultraweak, transient magnetic field changes.

    abstract::A novel method based on selective detection of rapidly changing DeltaB(0) magnetic fields and suppression of slowly changing DeltaB(0) fields is presented. The ultimate goal of this work is to present a method that may allow detection of transient and subtle changes in B(0) in cortical tissue associated with electrica...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10159

    authors: Bodurka J,Bandettini PA

    更新日期:2002-06-01 00:00:00

  • Abnormal brain anatomical topological organization of the cognitive-emotional and the frontoparietal circuitry in major depressive disorder.

    abstract:PURPOSE:Despite the increasing understanding of major depressive disorder (MDD) using neuroimaging techniques, the topological organization of anatomical networks underlying MDD remains unclear. METHODS:The topological organization of brain anatomical networks was explored using complex network approaches. Diffusion t...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25036

    authors: Qin J,Wei M,Liu H,Yan R,Luo G,Yao Z,Lu Q

    更新日期:2014-11-01 00:00:00

  • The effect of reducing repetition time TR on the measurement of liver R2 for the purpose of measuring liver iron concentration.

    abstract::The effects of reducing the pulse repetition time from 2500 ms to 1000 ms when using spin-density-projection-assisted R2-magnetic resonance imaging for the purpose of measuring liver iron concentration were evaluated. Repeated liver R2 measurements were made using both protocols on 60 subjects with liver iron concentr...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22712

    authors: Pavitt HL,Aydinok Y,El-Beshlawy A,Bayraktaroglu S,Ibrahim AS,Hamdy MM,Pang W,Sharples C,St Pierre TG

    更新日期:2011-05-01 00:00:00

  • Phase constrained encoding (PACE): a technique for MRI in large static field inhomogeneities.

    abstract::In spin echo imaging, magnetization is assigned to a location defined by its frequency of rotation. In the presence of a static magnetic field inhomogeneity, however, this location does not correspond to the true location of the magnetization. This paper describes a magnetic resonance imaging technique called phase co...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910330407

    authors: Kim JK,Plewes DB,Henkelman RM

    更新日期:1995-04-01 00:00:00

  • Spin-locking versus chemical exchange saturation transfer MRI for investigating chemical exchange process between water and labile metabolite protons.

    abstract::Chemical exchange saturation transfer (CEST) and spin-locking (SL) experiments were both able to probe the exchange process between protons of nonequivalent chemical environments. To compare the characteristics of the CEST and SL approaches in the study of chemical exchange effects, we performed CEST and SL experiment...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22721

    authors: Jin T,Autio J,Obata T,Kim SG

    更新日期:2011-05-01 00:00:00

  • Assessment and compensation of susceptibility artifacts in gradient echo MRI of hyperpolarized 3He gas.

    abstract::The effects of macroscopic background field gradients upon 2D gradient echo images of inhaled (3)He in the human lung were investigated at 1.5 T. Effective compensation of in-slice signal loss in (3)He gradient echo images was then demonstrated using a multiple acquisition interleaved single gradient echo sequence. Th...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10549

    authors: Wild JM,Fichele S,Woodhouse N,Paley MN,Swift A,Kasuboski L,van Beek EJ

    更新日期:2003-08-01 00:00:00

  • The field dependence of NMR imaging. II. Arguments concerning an optimal field strength.

    abstract::Some of the factors involved in the choice of field strength for NMR imaging are examined. The influences of relaxation times and chemical shift upon image quality and signal-to-noise ratio are highlighted, and power deposition is introduced as a significant factor which may limit the flexibility and information avail...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910030509

    authors: Hoult DI,Chen CN,Sank VJ

    更新日期:1986-10-01 00:00:00

  • Separation and quantification of lactate and lipid at 1.3 ppm by diffusion-weighted magnetic resonance spectroscopy.

    abstract:PURPOSE:To separate the spectrally overlapped lactate and lipid signals at 1.3 ppm using diffusion-weighted magnetic resonance spectroscopy (DW-MRS) based on their large diffusivity difference. METHODS:DW-MRS was applied to the gel phantoms containing lactate and lipid droplets, and to the rat brain tumors. Lactate an...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26144

    authors: Wang AM,Leung GK,Kiang KM,Chan D,Cao P,Wu EX

    更新日期:2017-02-01 00:00:00

  • A new design and rationale for 3D orthogonally oversampled k-space trajectories.

    abstract::A novel center-out 3D trajectory for sampling magnetic resonance data is presented. The trajectory set is based on a single Fermat spiral waveform, which is substantially undersampled in the center of k-space. Multiple trajectories are combined in a "stacked cone" configuration to give very uniform sampling throughout...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22918

    authors: Pipe JG,Zwart NR,Aboussouan EA,Robison RK,Devaraj A,Johnson KO

    更新日期:2011-11-01 00:00:00

  • Determining the extent to which delayed-enhancement images reflect the partition-coefficient of Gd-DTPA in canine studies of reperfused and unreperfused myocardial infarction.

    abstract::MRI after a constant infusion (CI) of Gd-DTPA has been used to identify the extent of myocardial infarction (MI). However, Gd-DTPA-enhanced "viability" imaging is more commonly performed with a bolus (for "delayed-enhancement" (DE) imaging). This study sought to determine how image delay time and time postinfarction i...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20236

    authors: Thornhill RE,Prato FS,Wisenberg G,Moran GR,Sykes J

    更新日期:2004-11-01 00:00:00

  • CEST, ASL, and magnetization transfer contrast: How similar pulse sequences detect different phenomena.

    abstract::Chemical exchange saturation transfer (CEST), arterial spin labeling (ASL), and magnetization transfer contrast (MTC) methods generate different contrasts for MRI. However, they share many similarities in terms of pulse sequences and mechanistic principles. They all use RF pulse preparation schemes to label the longit...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章,评审

    doi:10.1002/mrm.27341

    authors: Knutsson L,Xu J,Ahlgren A,van Zijl PCM

    更新日期:2018-10-01 00:00:00

  • pH mapping in living tissues: an application of in vivo 31P NMR chemical shift imaging.

    abstract::Chemical shifts were extracted from in vivo 3-dimensional 31P NMR CSI data and pH images were constructed. The images could spatially resolve tissue pH ranging from 5.8 to 7.2 (with uncertainty of 0.11-0.17 pH unit) in an ischemia-reperfusion model of diabetic rat calf muscles. ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910290214

    authors: Morikawa S,Inubushi T,Kito K,Kido C

    更新日期:1993-02-01 00:00:00

  • Magnetic resonance imaging of blood vessels at high fields: in vivo and in vitro measurements and image simulation.

    abstract::Unusually high image contrast in vivo magnetic resonance imaging of the brain becomes observable at high magnetic fields when the blood oxygenation level is lowered. The cause of the contrast has been attributed to a magnetic susceptibility effect induced by paramagnetic deoxyhemoglobin in red cells. When the cylinder...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910160103

    authors: Ogawa S,Lee TM

    更新日期:1990-10-01 00:00:00

  • In vivo measurements of bone marrow cellularity using volume-localized proton NMR spectroscopy.

    abstract::Volume-localized proton NMR spectroscopy was used to estimate bone marrow cellularity in the posterior iliac crests of patients undergoing treatment with hematopoietic growth factors for a variety of hematologic and neoplastic disorders. Twelve patients were accrued, six of whom were studied more than once, yielding a...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910190108

    authors: Ballon D,Jakubowski A,Gabrilove J,Graham MC,Zakowski M,Sheridan C,Koutcher JA

    更新日期:1991-05-01 00:00:00

  • Rapid T2 estimation with phase-cycled variable nutation steady-state free precession.

    abstract::Variable nutation SSFP (DESPOT2) permits rapid, high-resolution determination of the transverse (T2) relaxation constant. A limitation of DESPOT2, however, is the presence of T2 voids due to off-resonance banding artifacts associated with SSFP images. These artifacts typically occur in images acquired with long repeti...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20159

    authors: Deoni SC,Ward HA,Peters TM,Rutt BK

    更新日期:2004-08-01 00:00:00

  • Flow-suppressed hyperpolarized 13 C chemical shift imaging using velocity-optimized bipolar gradient in mouse liver tumors at 9.4 T.

    abstract:PURPOSE:To optimize and investigate the influence of bipolar gradients for flow suppression in metabolic quantification of hyperpolarized 13 C chemical shift imaging (CSI) of mouse liver at 9.4 T. METHODS:The trade-off between the amount of flow suppression using bipolar gradients and T2* effect from static spins was ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26578

    authors: Lee H,Lee J,Joe E,Yang S,Song JE,Choi YS,Wang E,Joo CG,Song HT,Kim DH

    更新日期:2017-11-01 00:00:00

  • Direct imaging of hyperpolarized 129Xe alveolar gas uptake in a mouse model of emphysema.

    abstract::MRI of hyperpolarized (129)Xe dissolved in pulmonary tissues, and blood has the potential to offer a new tool for regional evaluation of pulmonary gas exchange and perfusion; however, the extremely short T2* and low magnetization density make it difficult to acquire the image. In this study, an ultrashort echo-time se...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24452

    authors: Iguchi S,Imai H,Hori Y,Nakajima J,Kimura A,Fujiwara H

    更新日期:2013-07-01 00:00:00

  • Fe(III) distribution varies substantially within and between atherosclerotic plaques.

    abstract:PURPOSE:Vulnerable atherosclerotic plaques are structurally weak and prone to rupture, presumably due to local oxidative stress. Redox active iron is linked to oxidative stress and the aim of this study was to investigate the distribution of Fe(III) in carotid plaques and its relation to vulnerability for rupture. MET...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24687

    authors: Gustafsson H,Hallbeck M,Norell M,Lindgren M,Engström M,Rosén A,Zachrisson H

    更新日期:2014-02-01 00:00:00