Abstract:
:Seizures that occur during early development are associated with adverse neurodevelopmental outcomes. Causation and mechanisms are currently under investigation. Induction of an early life seizure by kainic acid (KA) in immature rats on post-natal day (P) 7 results in behavioral changes in the adult rat that reflect social and intellectual deficits without overt cellular damage. Our previous work also demonstrated increased expression of CA1 hippocampal long-term potentiation (LTP) and reduced desensitization of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type ionotropic glutamate receptors (AMPA-R) one week following a kainic acid induced seizure (KA-ELS). Here we used RNA sequencing (RNAseq) of mRNA from dorsal hippocampal CA1 to probe changes in mRNA levels one week following KA-ELS as a means to investigate the mechanisms for these functional changes. Ingenuity pathway analysis (IPA) confirmed our previous results by predicting an up-regulation of the synaptic LTP pathway. Differential gene expression results revealed significant differences in 7 gene isoforms. Additional assessments included AMPA-R splice variants and adenosine deaminase acting on RNA 2 (ADAR2) editing sites as a means to determine the mechanism for reduced AMPA-R desensitization. Splice variant analysis demonstrated that KA-ELS result in a small, but significant decrease in the "flop" isoform of Gria3, and editing site analysis revealed significant changes in the editing of a kainate receptor subunit, Grik2, and a serotonin receptor, Htr2c. While these specific changes may not account for altered AMPA-R desensitization, the differences indicate that KA-ELS alters gene expression in the hippocampal CA1 one week after the insult.
journal_name
Epilepsy Resjournal_title
Epilepsy researchauthors
O'Leary H,Vanderlinden L,Southard L,Castano A,Saba LM,Benke TAdoi
10.1016/j.eplepsyres.2020.106283subject
Has Abstractpub_date
2020-03-01 00:00:00pages
106283eissn
0920-1211issn
1872-6844pii
S0920-1211(19)30490-5journal_volume
161pub_type
杂志文章abstract::Traumatic brain injury (TBI) is a leading cause of symptomatic epilepsy in young adults. This study examined physiological and anatomical epileptogenic consequences of a prior incident of TBI in rats. Rats were subjected to a fluid percussion brain injury one week prior to experimentation, and in vitro electrophysiolo...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/s0920-1211(96)00044-7
更新日期:1996-12-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to investigate regional homogeneity (ReHo) in children with new-onset drug-naïve Benign Epilepsy with Centrotemporal Spikes (BECTS), chronic BECTS and healthy controls (HC) using the Regional Homogeneity (ReHo) method applied to resting state fMRI data. METHODS:Resting state fMR...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2015.06.017
更新日期:2015-10-01 00:00:00
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journal_title:Epilepsy research
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doi:10.1016/s0920-1211(02)00084-0
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pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2013.01.003
更新日期:2013-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2014.09.003
更新日期:2014-11-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/s0920-1211(98)00041-2
更新日期:1998-09-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2014.09.025
更新日期:2014-12-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/0920-1211(87)90031-3
更新日期:1987-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2019.02.002
更新日期:2019-03-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章,评审
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更新日期:2004-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2016.06.001
更新日期:2016-10-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/s0920-1211(97)00031-4
更新日期:1997-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2008.03.020
更新日期:2008-08-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2017.01.008
更新日期:2017-02-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2012.04.016
更新日期:2012-11-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2013.02.009
更新日期:2013-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章,评审
doi:10.1016/j.eplepsyres.2020.106429
更新日期:2020-10-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2020.106410
更新日期:2020-11-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2018.06.001
更新日期:2018-09-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/s0920-1211(00)00179-0
更新日期:2000-12-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2009.01.006
更新日期:2009-04-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2012.07.001
更新日期:2013-02-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2018.04.006
更新日期:2018-07-01 00:00:00
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journal_title:Epilepsy research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0920-1211(98)00015-1
更新日期:1998-06-01 00:00:00
abstract::The novel anticonvulsant, remacemide HCl [(+/-)-2-amino-N-(1-methyl-1,2-diphenylethyl)acetamide monohydrochloride; FPL12924AA], and a desglycinated metabolite [(+/-)-1-methyl-1,2-diphenylethylamine monohydrochloride; FPL 12495AA] reversibly limited sustained high-frequency repetitive firing (SRF) of sodium-dependent a...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/0920-1211(95)00053-4
更新日期:1996-02-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.eplepsyres.2018.07.004
更新日期:2018-10-01 00:00:00
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journal_title:Epilepsy research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.eplepsyres.2013.02.010
更新日期:2013-07-01 00:00:00
abstract::The effect of magnesium-free medium on electrical and CaM kinase II activity in the rat hippocampal slice was examined. Experimental slices were incubated in 2 mM Mg, then exposed to magnesium-free medium for 1 h. Control slices were concurrently run in 2 mM Mg. Slices were then frozen and CaM kinase II activity was m...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/0920-1211(91)90054-j
更新日期:1991-09-01 00:00:00
abstract::Increased prevalence of autoantibodies has been suggested in patients with epilepsy. This study determined the presence of autoantibodies in a representative cohort of 960 patients with epilepsy. The frequency of antinuclear antibodies (ANA), immunoglobulin G class anticardiolipin antibodies (aCL) and anti-B2-glycopro...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/j.eplepsyres.2003.12.004
更新日期:2004-01-01 00:00:00
abstract::The characteristics of evoked potentials in patients with dentatorubral-pallidoluysian atrophy (DRPLA) were investigated. Twelve patients with DRPLA and three patients with progressive myoclonic epilepsy (PME) attributable to other causes participated in the study. In 11 out of the 12 patients, the diagnosis of DRPLA ...
journal_title:Epilepsy research
pub_type: 杂志文章
doi:10.1016/s0920-1211(99)00028-5
更新日期:1999-10-01 00:00:00