Epigenetic biomarkers indicate islet cell death in xenotransplantation.

abstract：BACKGROUND:In vivo xenotransplantation modeling in large animal species is often performed in nonhuman primates, including baboons. For proper data interpretation, reference values for clinical chemistry and hematology are required. METHODS:These values are available from baseline levels in animals subjected to tolera...

journal_title：Xenotransplantation

authors： Schuurman HJ,Smith HT,Cozzi E

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:Despite overcoming xenograft hyperacute rejection (HAR), Gal (galactose-alpha1,3-galactose) expression may not be completely eliminated from the alpha1,3-galactosyltransferase gene knockout (Gal KO) pig because of alternative galactosyltransferases. Whether low levels of "residual" Gal are still susceptible ...

journal_title：Xenotransplantation

authors： Murray-Segal L,Gock H,Cowan PJ,d'Apice AJ

更新日期：2008-02-01 00:00:00

abstract：AIM:To provide information on the specificity of induced anti-pig antibodies (Abs) in baboons after exposure and sensitization to pig antigens. MATERIALS AND METHODS:Baboons (n=7) received either porcine mobilized peripheral blood progenitor cells (n=3), kidney (n=3) or heart (n=1) transplants. After rejection of thes...

journal_title：Xenotransplantation

authors： Buhler L,Xu Y,Li W,Zhu A,Cooper DK

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:Human cytomegalovirus (HCMV) infection or reactivation has been linked to allograft rejection resulting from endothelial injury and immune activation. In pig-to-human xenotransplantation, currently investigated to circumvent the shortage of human organs in transplantation medicine, the porcine endothelium wi...

journal_title：Xenotransplantation

authors： Millard AL,Häberli L,Sinzger C,Ghielmetti M,Schneider MK,Bossart W,Seebach JD,Mueller NJ

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND:We have successfully performed heart transplantation despite the most unfavourable risk factors for graft and patient survival: the presence of a high level of antibodies (Abs) against the donor's human leukocyte antigens (HLA) class I/II and blood group A1 antigens. The present study concerns post-transplan...

journal_title：Xenotransplantation

authors： Bućin D,Johansson S,Lindberg LO

更新日期：2006-03-01 00:00:00

abstract：:Xenotransplantion remains the most viable option for significant expansion of the donor organ pool in clinical transplantation. With the advent of nuclear transfer technologies, the production of transgenic swine has become a possibility. These animals have allowed transplant investigators to overcome humoral mechanis...

journal_title：Xenotransplantation

authors： Scalea J,Hanecamp I,Robson SC,Yamada K

更新日期：2012-01-01 00:00:00

abstract：BACKGROUND:As a step towards clinical cardiac xenotransplantation, our experimental heterotopic intrathoracic xenotransplantation model offers a beating and ejecting donor heart while retaining the recipient's native organ as a backup in case of graft failure. Clinically applicable immunosuppressive regimens (IS) were ...

journal_title：Xenotransplantation

authors： Abicht JM,Mayr T,Reichart B,Buchholz S,Werner F,Lutzmann I,Schmoeckel M,Bauer A,Thormann M,Langenmayer M,Herbach N,Pohla H,Herzog R,McGregor CG,Ayares D,Wolf E,Klymiuk N,Baehr A,Kind A,Hagl C,Ganswindt U,Belka C

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND:Porcine islet transplantation is emerging as an attractive option for the treatment of patients with type 1 diabetes, with the possibility of providing islets of higher and more consistent quality and in larger volumes than available from human pancreata. The use of encapsulated neonatal porcine islets (ENPI...

journal_title：Xenotransplantation

authors： Kitzmann JP,Law L,Shome A,Muzina M,Elliott RB,Mueller KR,Schuurman HJ,Papas KK

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:The aim of the present study was to further investigate the characteristics of both graft-infiltrating cells and splenocytes during acute vascular rejection (AVR), cell-mediated rejection and non-rejection of vascularized concordant xenografts, by analysing both proinflammatory [interleukin-1beta (IL-1beta) ...

journal_title：Xenotransplantation

authors： Lorant T,Krook H,Wilton J,Olausson M,Tufveson G,Korsgren O,Johnsson C

更新日期：2003-07-01 00:00:00

abstract：BACKGROUND:Dysregulation of coagulation is considered a major barrier against successful pig organ xenotransplantation in non-human primates. Inflammation is known to promote activation of coagulation. The role of pro-inflammatory factors as well as the relationship between inflammation and activation of coagulation in...

journal_title：Xenotransplantation

authors： Ezzelarab MB,Ekser B,Azimzadeh A,Lin CC,Zhao Y,Rodriguez R,Echeverri GJ,Iwase H,Long C,Hara H,Ayares D,Pierson RN,Thomson AW,Cooper DK

更新日期：2015-01-01 00:00:00

abstract：:Islet transplantation is a potential treatment for diabetes mellitus and porcine pancreata may provide a readily available source of islets. The size, number and distribution of islets within the pancreas may influence the choice of age of donor for xenotransplantation. Samples (n = 3 per age group) from the dorsal an...

journal_title：Xenotransplantation

authors： Jay TR,Heald KA,Carless NJ,Topham DE,Downing R

更新日期：1999-05-01 00:00:00

abstract：:The first blood transfusions in humans were xenotransfusions, carried out by Jean-Baptiste Denis beginning in 1667. Richard Lower, Matthäus Purmann and Georges Mercklin also experimented with the use of animal blood for transfusion until this practice was forbidden in 1670, after the death of one of Denis's patients. ...

journal_title：Xenotransplantation

authors： Roux FA,Saï P,Deschamps JY

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Swine infectious pathogens, especially viruses, represent a potential public health risk associated with the use of pig tissues for xenotransplantation in humans. We hypothesized that porcine circovirus type I (PCV-1) may infect human mononuclear cells, resulting in ultrastructural alterations of the target ...

journal_title：Xenotransplantation

authors： Arteaga-Troncoso G,Guerra-Infante F,Rosales-Montaño LM,Díaz-García FJ,Flores-Medina S

更新日期：2005-11-01 00:00:00

abstract：:We investigated the predictive biomarkers for graft rejection in pig-to-non-human primate (NHP) full-thickness corneal xenotransplantation (n = 34). The graft score (0-12) was calculated based on opacity, edema, and vascularization. Scores ≥ 6 were defined as rejection. NHPs were divided into two groups: (a) graft rej...

journal_title：Xenotransplantation

authors： Yoon CH,Choi SH,Lee HJ,Kang HJ,Kim MK

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:Type 1 diabetes mellitus is a devastating disease for which there is currently no cure, but only lifetime management. Islet xenotransplantation is a promising technique for the restoration of blood glucose control in patients with diabetes mellitus. The purpose of this study was to explore the potential use ...

journal_title：Xenotransplantation

authors： Hani H,Allaudin ZN,Mohd-Lila MA,Ibrahim TA,Othman AM

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Liver fibrosis results from accumulation of extracellular matrix components and is associated with many chronic hepatic diseases. There is to date no specific therapy for this disease, and patients receive treatment for its associated complications. Specific progenitor cells, known as oval cells, are present...

journal_title：Xenotransplantation

authors： Sáez-Lara MJ,Frecha C,Martín F,Abadía F,Toscano M,Gil A,Fontana L

更新日期：2006-11-01 00:00:00

abstract：:Cultures of hDAF transgenic porcine aortic endothelial cells (TPAEs) with levels of cell surface hDAF expression between 2000 and 300 000 molecules per cell have been used to determine the relationship between expression of hDAF and protection from human complement deposition in an in vitro model. At concentrations be...

journal_title：Xenotransplantation

authors： Carrington CA,dos Santos Cruz G

更新日期：2001-05-01 00:00:00

abstract：:Development of coagulation disorders remains a major challenge in pig-to-primate organ xenotransplantation. Our previous studies demonstrated that porcine aortic endothelial cells (pAEC) activate human platelets to express tissue factor (TF). In this study, we investigated the molecular interaction between human plate...

journal_title：Xenotransplantation

authors： Ezzelarab MB,Liu YW,Lin CC,Long C,Ayares D,Dorling A,Cooper DK

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Sertoli cells (SC) are known to have active mechanism for evading humoral immune response and are known to have immune modulatory effects in the presence of other antigens. This has led us to hypothesize that systemic immune modulating effect of SC might be optimized by their residence on peripheral lymph no...

journal_title：Xenotransplantation

authors： Lee HM,Lim HG,Oh BC,Park CS,Lee DS,Lee JR

更新日期：2007-11-01 00:00:00

abstract：BACKGROUND:Blood group ABO-incompatible live donor (LD) renal transplantation may provide a significant source of organs. We report the results of our first 14 cases of ABO-incompatible LD renal transplantation using specific anti-A/B antibody (Ab) immunoadsorption (IA) and anti-CD20 monoclonal Ab (mAb) treatment. PATI...

journal_title：Xenotransplantation

authors： Nordén G,Briggs D,Cockwell P,Lipkin G,Mjörnstedt L,Mölne J,Ready A,Rydberg L,Samuelsson O,Svalander CT,Breimer ME

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND AND AIMS:Cell-based therapies for liver disease such as bioartificial liver rely on a large quantity and high quality of hepatocytes. Cold storage was previously shown to be a better way to preserve the viability and functionality of hepatocytes during transportation rather than freezing, but this was only p...

journal_title：Xenotransplantation

authors： Li Y,Chen HS,Shaheen M,Joo DJ,Amiot BP,Rinaldo P,Nyberg SL

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:ABI793 (ABI) is a human monoclonal antibody (mAb) specific for human CD154. To assess the suitability of ABI for baboon transplantation studies, we carried out in vitro studies to determine ABI's reactivity with baboon cells expressing CD154, performed in vivo pharmacokinetic studies in two baboons, and test...

journal_title：Xenotransplantation

authors： Knosalla C,Ryan DJ,Moran K,Gollackner B,Schuler W,Sachs DH,Awwad M,Schuurman HJ,Cooper DK

更新日期：2004-07-01 00:00:00

abstract：:Complement plays a major role in hyperacute rejection of xenografts. In order to overcome this, we are developing, by minimal mutagenesis, a modified C3 molecule that, like cobra venom factor (CVF), escapes normal complement regulatory processes and inhibits complement-mediated responses by systemic depletion of C3. U...

journal_title：Xenotransplantation

authors： Fecke W,Farries TC,D'Cruz LG,Napper CM,Harrison RA

更新日期：1998-02-01 00:00:00

abstract：BACKGROUND:Innovations in transgenic technology have facilitated improved xenograft survival. Additional gene expression appears to be necessary to overcome the remaining immune and biologic incompatibilities. We report for the first time the novel use of six-gene modifications within a pig-to-baboon cardiac xenotransp...

journal_title：Xenotransplantation

authors： Chan JL,Singh AK,Corcoran PC,Thomas ML,Lewis BG,Ayares DL,Vaught T,Horvath KA,Mohiuddin MM

更新日期：2017-11-01 00:00:00

abstract：:Although xenografts have always held immeasurable potential as an inexhaustible source of donor organs, immunological barriers and physiological incompatibility have proved to be formidable obstacles to clinical utility. An exciting, new regenerative medicine-based approach termed "semi-xenotransplantation" (SX) seeks...

journal_title：Xenotransplantation

authors： Salvatori M,Peloso A,Katari R,Soker S,Lerut JP,Stratta RJ,Orlando G

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:TNF and its receptors TNF-Receptor 1 (TNFR1, CD120a) and TNF-Receptor 2 (TNFR2, CD120b) have been implicated in the rejection of transplanted cells and organs. Although pig TNFR1 (pTNFR1) is known to mediate the effects of human TNF in a xenogeneic setting, it is unclear whether pig TNFR2 (pTNFR2) could cont...

journal_title：Xenotransplantation

authors： Uribe-Herranz M,Casinghino SR,Bosch-Presegué L,Fodor WL,Costa C

更新日期：2011-03-01 00:00:00

abstract：BACKGROUND:Little information is available regarding the precise swine leukocyte antigen (SLA)-derived immunogenic peptides that are presented in the context of human HLA molecules. Here, we identified SLA-derived immunogenic peptides that are presented in association with human HLA-A2 molecule. METHODS:The SLA-derive...

journal_title：Xenotransplantation

authors： Park CS,Im SA,Song S,Kim K,Lee CK

更新日期：2014-09-01 00:00:00

abstract：:The present study was undertaken to establish a rat-to-mouse vascularized small bowel xenotransplantation model to study acute vascular and hyperacute xenograft rejection, and xenogenic cell migration. Lewis rat small bowel grafts were transplanted heterotopically to group 1, Balb/c mice, and group 2, Balb/c mice pre-...

journal_title：Xenotransplantation

authors： Kiyochi H,Kellersmann R,Blömer A,Garcia BM,Zhang Z,Zhong R,Grant DR

更新日期：1999-02-01 00:00:00

abstract：BACKGROUND:The pig pancreas is considered to be the most suitable source of islets for xenotransplantation in patients with type I diabetes. The objective of this study was to assess the antigenicity of neonatal porcine islet-like cell clusters (NPCC), including the Galalpha1-3Galbeta1-4GlcNAc-R (alpha-Gal) and Hanganu...

journal_title：Xenotransplantation

authors： Omori T,Nishida T,Komoda H,Fumimoto Y,Ito T,Sawa Y,Gao C,Nakatsu S,Shirakura R,Miyagawa S

更新日期：2006-09-01 00:00:00

abstract：:Type 1 diabetes can be cured by transplantation of isolated pancreatic islets. Because of the shortage of human donor tissue, adult porcine islets (APIs) constitute a possible alternative tissue source. Upon intraportal injection, islets are subjected to an instant blood-mediated inflammatory reaction (IBMIR) leading ...

journal_title：Xenotransplantation

authors： Ehrnfelt C,Kumagai-Braesch M,Uzunel M,Holgersson J

更新日期：2004-03-01 00:00:00