Abstract：
:The features of B16 melanoma progression in male C57BL/6 mice with initially high and low resistance to hypoxia were studied. To assess the resistance to hypoxia, the mice were placed in a low-pressure chamber at a simulated altitude of 10,000 m. One month after testing, B16 melanoma was inoculated to high- and low-resistant animals. In 19 days after melanoma transplantation, the severity of melanoma progression was assessed by morphological and immunofluorescent methods. The expression of vegf-a and hif-1a in the liver of melanomabearing and control mice was evaluated by real-time PCR. Tumor growth progression was more pronounced in low-resistant mice, which was seen from high weight of the primary tumor node, relative necrosis area, proliferation rates (mitotic index and number of Ki-67+ cells), and expression of vegf-a gene in the liver. In high-resistant to hypoxia animals, the number of caspase-3+ cells dying by apoptosis was higher. The data on more rapid melanoma progression in mice with low resistance to hypoxia should be considered during the search of new prognostic markers and methods for therapy of malignant neoplasms.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Fridman IA,Ponomarenko EA,Makarova OV,Postovalova EA,Zolotova NA,Khochanskiy DN,Mkhitarov VA,Tsvetkov IS,Kosyreva AMdoi
10.1007/s10517-020-04716-wsubject
Has Abstractpub_date
2020-01-01 00:00:00pages
390-394issue
3eissn
0007-4888issn
1573-8221pii
10.1007/s10517-020-04716-wjournal_volume
168pub_type
杂志文章abstract：:Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7(+) epithelial cells that disappeared by passage 2. The cultures from the endometriu...
journal_title：Bulletin of experimental biology and medicine
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abstract：:In experiments on Wistar rats we studied the effect of low-frequency electromagnetic field rotating in either right-handed or a left-handed sense on proteolytic activity of pepsin. The right-handed rotating field increased, while left-handed rotating field decreased pepsin activity. Possible mechanisms of these change...
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