Vitreous albumin redox state in open-angle glaucoma patients and controls: a pilot study.

Abstract:

PURPOSE:Numerous studies suggest that reactive oxygen species play a crucial role in the development of glaucoma. Since glaucoma patients exhibit posterior vitreous detachment earlier than controls, it has been suggested that reactive oxygen species-increased in glaucoma-also affect the vitreous. In the present study we evaluated the influence of open-angle glaucoma oxidative stress on the redox state of vitreous albumin. METHODS:Albumin redox states of the vitreous and plasma were evaluated in 22 subjects-11 open-angle glaucoma patients and 11 controls-matched for age, gender, and vitreous state. According to the redox state of cysteine-34, albumin can be separated into: human mercaptalbumin (the thiol form), human nonmercaptalbumin1 (a reversible modification due to mild oxidation), and human nonmercaptalbumin2 (an irreversible modification due to severe oxidation). RESULTS:Albumin of both, the open-angle glaucoma group and the control group, was more oxidized in the vitreous compared to plasma. Furthermore, significantly higher human nonmercaptalbumin1 fractions were found in the vitreous of open-angle glaucoma patients compared to controls. No significant differences were found in the plasma albumin fractions between the groups. CONCLUSION:Our results support the hypothesis that oxidative stress plays a crucial role in open-angle glaucoma and that reactive oxygen species in glaucomatous eyes may also affect the vitreous.

journal_name

Int Ophthalmol

authors

Schwab C,Paar M,Fengler VH,Lindner E,Haas A,Ivastinovic D,Seidel G,Weger M,Wedrich A,Oettl K

doi

10.1007/s10792-019-01268-5

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

999-1006

issue

4

eissn

0165-5701

issn

1573-2630

pii

10.1007/s10792-019-01268-5

journal_volume

40

pub_type

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