Manganese Chloride Exposure Causes Disorder of Energy Metabolism and Induces Oxidative Stress and Autophagy in Chicken Liver.

Abstract:

:Manganese (Mn) pollution is an important environmental problem because of the potential toxicity to human and animal health. However, the effects of Mn on energy metabolism and autophagy are not clear. Consequently, we examined the effects of excessive and chronic exposure to Mn on liver function, oxidative stress, respiratory chain complex activity, and autophagy in chicken liver. Our results indicated that the accumulation of Mn in the liver and levels of AST and ALT in the serum of the Mn-exposed group were significantly higher (P < 0.05) than those in the control group at 90 days; the activities of GSH-Px, SOD, CAT, Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and respiratory chain complexes (I, II, III) in the Mn-exposed group were significantly decreased (P < 0.05) compared to the control group. However, the MDA content, NO content, iNOS activity, mRNA and protein levels of iNOS, and autophagy-related genes in the Mn-exposed group were significantly increased (P < 0.05) compared to the control group. In contrast, the mRNA level and protein expression of mTOR were significantly decreased (P < 0.05) compared to the control group. Furthermore, the characteristic autophagic vacuolar organelles were observed in the Mn-exposed group. These results suggested that excess Mn exposure can cause a disorder of energy metabolism by mitochondrial injury and induce oxidative stress and autophagy, which eventually lead to liver damage.

journal_name

Biol Trace Elem Res

authors

Jiang J,Wang F,Wang L,Xiao J,Guo D

doi

10.1007/s12011-019-01960-8

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

254-261

issue

1

eissn

0163-4984

issn

1559-0720

pii

10.1007/s12011-019-01960-8

journal_volume

197

pub_type

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