当前位置： SCI文献检索 > VIROLOGICA SINICA期刊下所有文献 > Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.

Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.

Abstract：

:Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33°C and 35°C was carried out by shifting the culture temperature between 28°C and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1-4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28°C, 33°C, 35°C and 37°C, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.

journal_name

Virol Sin

journal_title

Virologica Sinica

authors

Wu YL,Jiang L,Hashimoto Y,Granados RR,Li GX

doi

10.1007/s12250-011-3177-x

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

198-205

issue

3

eissn

1674-0769

issn

1995-820X

journal_volume

26

pub_type

杂志文章

相关文献

VIROLOGICA SINICA文献大全
  • SRP54 Negatively Regulates IFN-Beta Production and Antiviral Response by Targeting RIG-I and MDA5.

    abstract：:During virus infection, RIG-I-like receptors (RLRs) recognize viral RNAs and recruit the adaptor protein VISA to activate downstream signaling, leading to activation of transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs). IFNs further induce expression of hundreds of IFN-stimula...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-020-00267-6

    authors： Wang DP,Zhang HY,Liao BW,Tong Z,Xu ZS,Wang YY,Yang Y

    更新日期：2020-08-07 00:00:00

  • Bovine herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cells.

    abstract：:The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1's infection could repress the basal or inducible transcription ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-011-3213-x

    authors： Liu C,Kong XH,Qiao WT,Geng YQ

    更新日期：2011-12-01 00:00:00

  • Interplay between hepatitis B virus and the innate immune responses: implications for new therapeutic strategies.

    abstract：:Hepatitis B virus (HBV) infection is still a worldwide health problem; however, the current antiviral therapies for chronic hepatitis B are limited in efficacy. The outcome of HBV infection is thought to be the result of complex interactions between the HBV and the host immune system. While the role of the adaptive im...

    journal_title：Virologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1007/s12250-014-3412-3

    authors： Chen J,Yuan Z

    更新日期：2014-02-01 00:00:00

  • Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China.

    abstract：:The dengue virus (DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics o...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-016-3872-8

    authors： Li G,Pan P,He Q,Kong X,Wu K,Zhang W,Liu Y,Huang H,Liu J,Zhang Z,Wu,Lai X,Liu X,Wu J

    更新日期：2017-02-01 00:00:00

  • The Saffold Virus-Penang 2B and 3C Proteins, but not the L Protein, Induce Apoptosis in HEp-2 and Vero Cells.

    abstract：:Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of S...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-019-00116-1

    authors： Xu Y,Victorio CBL,Meng T,Jia Q,Tan YJ,Chua KB

    更新日期：2019-06-01 00:00:00

  • Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process.

    abstract：:Exosomes are cell-derived vesicles that are secreted by many eukaryotic cells. It has recently attracted attention as vehicles of intercellular communication. Virus-infected cells release exosomes, which contain viral proteins, RNA, and pathogenic molecules. However, the role of exosomes in virus infection process rem...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-019-00087-3

    authors： Wang J,Wu F,Liu C,Dai W,Teng Y,Su W,Kong W,Gao F,Cai L,Hou A,Jiang C

    更新日期：2019-02-01 00:00:00

  • Detection of congenital cytomegalovirus in newborns using nucleic acid amplification techniques and its public health implications.

    abstract：:Human cytomegalovirus (HCMV), a herpesvirus, is an important human pathogen that causes asymptomatic infections in healthy or immunocompetent individuals but can lead to severe and potentially life-threatening complications in immune-immature individuals such as neonates or immune-compromised patients such as organ-tr...

    journal_title：Virologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1007/s12250-017-4055-y

    authors： Liu G,Hai R,Liu F

    更新日期：2017-10-01 00:00:00

  • Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin.

    abstract：:The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we pr...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-016-3742-4

    authors： Lei J,Hilgenfeld R

    更新日期：2016-08-01 00:00:00

  • Ion channels as antivirus targets.

    abstract：:Ion channels are membrane proteins that are found in a number of viruses and which are of crucial physiological importance in the viral life cycle. They have one common feature in that their action mode involves a change of electrochemical or proton gradient across the bilayer lipid membrane which modulates viral or c...

    journal_title：Virologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1007/s12250-010-3136-y

    authors： Liang X,Li ZY

    更新日期：2010-08-01 00:00:00

  • Histone Deacetylase 3 Inhibitor Suppresses Hepatitis C Virus Replication by Regulating Apo-A1 and LEAP-1 Expression.

    abstract：:Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-018-0057-7

    authors： Zhou Y,Wang Q,Yang Q,Tang J,Xu C,Gai D,Chen X,Chen J

    更新日期：2018-10-01 00:00:00

  • Isolation and identification of a subgroup A avian leukosis virus from imported meat-type grand-parent chickens.

    abstract：:An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-010-3095-3

    authors： Zhang QC,Zhao DM,Guo HJ,Cui ZZ

    更新日期：2010-04-01 00:00:00

  • Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived (Huh7) cells.

    abstract：:Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are the limiting factors ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-017-3983-x

    authors： Zhou M,Zhao K,Yao Y,Yuan Y,Pei R,Wang Y,Chen J,Hu X,Zhou Y,Chen X,Wu C

    更新日期：2017-12-01 00:00:00

  • The Prevalence, Genetic Characterization, and Evolutionary Analysis of Porcine Pegivirus in Guangdong, China.

    abstract：:Porcine pegivirus (PPgV) is a member of the Pegivirus genus in the Flaviviridae family. PPgV is an emerging virus that has been discovered in swine herds in Germany, the United States, China, Poland, Italy, and the United Kingdom, indicating a wide geographical distribution. In this retrospective study, 339 pig serum ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-020-00240-3

    authors： Xie Y,Wang X,Feng J,Wei L,Li G,Si G,Chen Y,Yan H,He D

    更新日期：2020-07-08 00:00:00

  • Establishment of an ELISA to detect Kaposi's sarcoma-associated herpesvirus using recombinant ORF73.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi's sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman's disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was iden...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-010-3106-4

    authors： Ouyang XX,Fu BS,Li BL,Zeng Y,Xu FH,Wang LD

    更新日期：2010-06-01 00:00:00

  • Transcriptional regulation by HSV-1 induced HTRP via acetylation system.

    abstract：:The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP30 (mSin3A Association Protein), one of the components of co-repressor complex mSin3A, which is part of the ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-010-3147-8

    authors： Chen J,Li YM,Li JF,Liu LD,Liao Y,Na RX,Wang JJ,Wang LC,Li QH

    更新日期：2010-12-01 00:00:00

  • Characterization of pigeon paramyxoviruses (Newcastle disease virus) isolated in Kazakhstan in 2005.

    abstract：:Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the ampl...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-012-3234-0

    authors： Bogoyavlenskiy A,Berezin V,Prilipov A,Usachev E,Korotetskiy I,Zaitceva I,Kydyrmanov A,Sayatov M

    更新日期：2012-04-01 00:00:00

  • Highly pathogenic avian influenza H5N1 Clade 2.3.2.1c virus in migratory birds, 2014-2015.

    abstract：:A novel Clade 2.3.2.1c H5N1 reassortant virus caused several outbreaks in wild birds in some regions of China from late 2014 to 2015. Based on the genetic and phylogenetic analyses, the viruses possess a stable gene constellation with a Clade 2.3.2.1c HA, a H9N2-derived PB2 gene and the other six genes of Asian H5N1-o...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-016-3750-4

    authors： Bi Y,Chen J,Zhang Z,Li M,Cai T,Sharshov K,Susloparov I,Shestopalov A,Wong G,He Y,Xing Z,Sun J,Liu D,Liu Y,Liu L,Liu W,Lei F,Shi W,Gao GF

    更新日期：2016-08-01 00:00:00

  • Prediction of the Receptorome for the Human-Infecting Virome.

    abstract：:The virus receptors are key for the viral infection of host cells. Identification of the virus receptors is still challenging at present. Our previous study has shown that human virus receptor proteins have some unique features including high N-glycosylation level, high number of interaction partners and high expressi...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-020-00259-6

    authors： Zhang Z,Ye S,Wu A,Jiang T,Peng Y

    更新日期：2020-07-28 00:00:00

  • The Scorpion Venom Peptide Smp76 Inhibits Viral Infection by Regulating Type-I Interferon Response.

    abstract：:Dengue virus (DENV) and Zika virus (ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-018-0068-4

    authors： Ji Z,Li F,Xia Z,Guo X,Gao M,Sun F,Cheng Y,Wu Y,Li W,Ali SA,Cao Z

    更新日期：2018-12-01 00:00:00

  • Chevrier's Field Mouse (Apodemus chevrieri) and Père David's Vole (Eothenomys melanogaster) in China Carry Orthohepeviruses that form Two Putative Novel Genotypes Within the Species Orthohepevirus C.

    abstract：:Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 specimens collected from seven wild s...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-018-0011-8

    authors： Wang B,Li W,Zhou JH,Li B,Zhang W,Yang WH,Pan H,Wang LX,Bock CT,Shi ZL,Zhang YZ,Yang XL

    更新日期：2018-02-01 00:00:00

  • Foot-and-Mouth Disease Virus Inhibits RIP2 Protein Expression to Promote Viral Replication.

    abstract：:Receptors interaction protein 2 (RIP2) is a specific adaptor molecule in the downstream of NOD2. The role of RIP2 during foot-and-mouth disease virus (FMDV) infection remains unknown. Here, our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-β and NF-ĸB signal ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-020-00322-2

    authors： Liu H,Xue Q,Zhu Z,Yang F,Cao W,Liu X,Zheng H

    更新日期：2021-01-05 00:00:00

  • Flu Universal Vaccines: New Tricks on an Old Virus.

    abstract：:Conventional influenza vaccines are based on predicting the circulating viruses year by year, conferring limited effectiveness since the antigenicity of vaccine strains does not always match the circulating viruses. This necessitates development of universal influenza vaccines that provide broader and lasting protecti...

    journal_title：Virologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1007/s12250-020-00283-6

    authors： Du R,Cui Q,Rong L

    更新日期：2020-09-01 00:00:00

  • HearNPV Pseudotyped with PIF1, 2, and 3 from MabrNPV: Infectivity and Complex Stability.

    abstract：:Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-018-0014-5

    authors： Makalliwa GA,Wang X,Zhang H,Zhang N,Chen C,Li J,Deng F,Wang H,Wang M,Hu Z

    更新日期：2018-04-01 00:00:00

  • Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes.

    abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the i...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-020-00289-0

    authors： Wu X,Li M,Zhang Y,Liang B,Shi J,Fang Y,Su Z,Li M,Zhang W,Xu L,Wang J,Wu Q,Tang S,Wang H,Zhang T,Peng C,Zheng X,Deng F,Shen S

    更新日期：2020-09-22 00:00:00

  • The identification of three sizes of core proteins during the establishment of persistent hepatitis C virus infection in vitro.

    abstract：:Similar to Hepatitis C virus (HCV) infection in humans, HCVcc infection can also result in persistent and chronic infection. The core protein is a variable protein and exists in several sizes. Some sizes of core proteins have been reported to be related to chronic HCV infection. To study the possible role of the core ...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-013-3296-7

    authors： Liao Q,Tian J,Wu Y,Chen X

    更新日期：2013-06-01 00:00:00

  • Study on passive immunity: Time of vaccination in kids born to goats vaccinated against Peste des petits ruminants.

    abstract：:In this study, the decay of maternal peste des petits ruminants virus (PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids. Serum samples collected from kids born t...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-012-3249-6

    authors： Balamurugan V,Sen A,Venkatesan G,Rajak KK,Bhanuprakash V,Singh RK

    更新日期：2012-08-01 00:00:00

  • A case of hepatitis B reactivation due to the hepatitis B virus escape mutant in a patient undergoing chemotherapy.

    abstract：:A 62-year-old man had chronic hepatitis B virus (HBV) infection and was diagnosed with liver cirrhosis. At the time of diagnosis the patient's virologic markers were positive for hepatitis B surface antigen (HBsAg), antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc), while...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-012-3284-3

    authors： Wu C,Shi H,Wang Y,Lu M,Xu Y,Chen X

    更新日期：2012-12-01 00:00:00

  • A Crowned Killer's Résumé: Genome, Structure, Receptors, and Origin of SARS-CoV-2.

    abstract：:The recent emergence and rapid global spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pose an unprecedented medical and socioeconomic crisis, and the disease caused by it, Coronavirus disease 2019 (COVID-19), was declared a pandemic by the World Health Organization (WHO) on March 11, 2020. C...

    journal_title：Virologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1007/s12250-020-00298-z

    authors： Wang S,Trilling M,Sutter K,Dittmer U,Lu M,Zheng X,Yang D,Liu J

    更新日期：2020-12-01 00:00:00

  • B cell epitopes within VP1 of type O foot-and-mouth disease virus for detection of viral antibodies.

    abstract：:In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 (epitope1), tandem repeat 200-213 (epitope2 (+2)) and the combination of two epitopes (epitope1-2) was genetically cloned into the prokaryotic expression vector pP...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-010-3041-4

    authors： Gao SD,Du JZ,Chang HY,Cong GZ,Shao JJ,Lin T,Song S,Xie QG

    更新日期：2010-02-01 00:00:00

  • Extensive evolution analysis of the global chikungunya virus strains revealed the origination of CHIKV epidemics in Pakistan in 2016.

    abstract：:Chikungunya virus (CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mo...

    journal_title：Virologica Sinica

    pub_type： 杂志文章

    doi：10.1007/s12250-017-4077-5

    authors： Shi J,Su Z,Fan Z,Wang J,Liu S,Zhang B,Wei H,Jehan S,Jamil N,Shen S,Deng F

    更新日期：2017-12-01 00:00:00