Abstract:
:Recent evidence suggests that the canonical miRNA processing pathway can be regulated by a number of positive and negative trans-acting factors. This chapter provides an overview of hnRNP Al-mediated regulation of miR-18a biogenesis. Our laboratory has recently established that the multifunctional RNA-binding protein hnRNP Al is required for the processing of miR-18a at the nuclear step of Drosha-mediated processing. By combining structural and functional analysis of RNA, we showed that hnRNP Al regulates the processing of pri-miR-18a by binding to its terminal loop and reshaping its stem-loop structure, thus allowing for a more effective Drosha cleavage. Furthermore, we linked the event of hnRNP A1-binding to the pri-miR-18a with an unusual phylogenetic sequence conservation of its terminal loop. Bioinformatic and mutational analysis revealed that a number of pri-miRNAs have highly conserved terminal loops, which are predicted to act as landing pads for trans-acting factors influencing miRNA processing. These results underscore a previously uncharacterized role for general RNA-binding proteins as factors that facilitate the processing of specific miRNAs, revealing an additional level of complexity for the regulation of miRNA production and function.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Michlewski G,Guil S,Cáceres JFdoi
10.1007/978-1-4419-7823-3_3subject
Has Abstractpub_date
2011-01-01 00:00:00pages
28-35eissn
0065-2598issn
2214-8019journal_volume
700pub_type
杂志文章,评审abstract::In sexual reproduction in humans, a man has a clear interest in ensuring that the immune system of his female partner accepts the semi-allogenic fetus. Increasing attention has been given to soluble immunomodulatory molecules in the seminal fluid as one mechanism of ensuring this, possibly by "priming" the woman's imm...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-18881-2_8
更新日期:2015-01-01 00:00:00
abstract::Late-stage age-related macular degeneration (AMD) is a common sight-threatening disease of the central retina affecting approximately 1 in 30 Caucasians. Besides age and smoking, common genetic variants from at least 19 gene loci have reproducibly been associated with AMD likely explaining a large proportion of diseas...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4614-3209-8_37
更新日期:2014-01-01 00:00:00
abstract::An appropriate animal model is essential to screening drugs or designing a treatment strategy for geographic atrophy. Since oxidative stress contributes to the pathological changes of the retinal pigment epithelium (RPE), we are reporting a new mouse AMD model of retinal degeneration by inducing mitochondrial oxidativ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-17121-0_5
更新日期:2016-01-01 00:00:00
abstract::The use of targeted proteomics to identify urinary biomarkers of kidney disease in urine can avoid the interference of serum proteins. It may provide better sample throughput, higher sensitivity, and specificity. Knowing which urinary proteins to target is essential for targeted proteomics. In perfusates, there were p...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-94-017-9523-4_17
更新日期:2015-01-01 00:00:00
abstract::Clostridium difficile infection (CDI) remains a considerable challenge to health care systems worldwide. Although CDI represents a significant burden on healthcare systems in Europe, few studies have attempted to estimate the consumption of resources associated with CDI in Europe. The reported extra costs attributable...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-72799-8_1
更新日期:2018-01-01 00:00:00
abstract::Neonatal stroke presents with features of encephalopathy and can result in significant morbidity and mortality. We investigated the cerebral metabolic and haemodynamic changes following neonatal stroke in a term infant at 24 h of life. Changes in oxidation state of cytochrome-c-oxidase (oxCCO) concentration were monit...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4939-3023-4_62
更新日期:2016-01-01 00:00:00
abstract::Small angle scattering (SAS) is a powerful and versatile tool to elucidate the structure of matter at the nanometer scale. Recently, the technique has seen a tremendous growth of applications in the field of structural molecular biology. Its origins however date back to almost a century ago and even though the methods...
journal_title:Advances in experimental medicine and biology
pub_type: 历史文章,杂志文章,评审
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更新日期:2017-01-01 00:00:00
abstract::The "silent epidemic" of Chlamydia trachomatis threatens to cause reproductive damage and infertility in many of the 50 million women who acquire it each year. Female reproductive tract infection has more recently been linked to stillbirth and premature delivery. Innate immune cells and mediators appear to be the prim...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4614-4726-9_8
更新日期:2013-01-01 00:00:00
abstract::Screening of recombinant cosmid and phage libraries with a rabbit C alpha cDNA probe has identified a total of seven C alpha genes organized into two clusters; one cluster contains four genes and the other cluster, three genes. Southern blot analysis of genomic DNA indicates that the rabbit genome contains a minimum o...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-5344-7_3
更新日期:1987-01-01 00:00:00
abstract::In this paper we report the effect of sulfatides on rate constants of Factor XII activation by kallikrein and its isolated light chain. In free solution kallikrein and the light chain were equally effective in activating Factor XII and both enzymes had their pH optimum at pH 7.0 (k1 = 1.6 X 10(3) M-1 s-1). Sulfatides ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-0154-8_3
更新日期:1986-01-01 00:00:00
abstract::Tumors consists of subpopulation of cells in which each subtype has contributes to tumor progression. Specifically one subtype known as cancer stem cells are associated with the initiation, progression, resistance to conventional therapies and metastasis. Metastasis is leading cause of cancer related deaths. Overall i...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/5584_2018_279
更新日期:2018-01-01 00:00:00
abstract::Pertinent data from the literature and in press is summarised and used to construct a model for the molecular arrangement of lipid and protein in the lamellae of compact central nerve myelin. For the lipid phase of myelin the available data is best interpreted in terms of a bilayer arrangement while physical studies s...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-2514-7_17
更新日期:1978-01-01 00:00:00
abstract::Advancements in musculoskeletal analysis have been achieved by adopting deep learning technology in image recognition and analysis. Unlike musculoskeletal modeling based on computational anatomy, deep learning-based methods can obtain muscle information automatically. Through analysis of image features, both approache...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-33128-3_11
更新日期:2020-01-01 00:00:00
abstract::Progress in studies on the scrapie agent has been hampered by the slow and tedious endpoint titration assays in rodents. A new assay based on incubation period measurements has been developed. The incubation period is defined as the time interval from inoculation to the onset of clinically detectable neurological illn...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-0495-2_35
更新日期:1981-01-01 00:00:00
abstract::The decrease in adiponectin levels are negatively correlated with chronic subclinical inflammation markers in obesity. The hypertrophic adipocytes cause obesity-linked insulin resistance and metabolic syndrome. Furthermore, macrophage polarization is a key determinant regulating adiponectin receptor (AdipoR1/R2) expre...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-48382-5_18
更新日期:2017-01-01 00:00:00
abstract::Both hepatic lipogenesis from glucose and gluconeogenesis from lactate are inhibited by substrates for mixed function oxidation such as aminopyrine. These effects are inducible with phenobarbital pretreatment and absent with a demethylated product of aminopyrine metabolism, aminophenylpyrazolone. The data indicate tha...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-9026-2_25
更新日期:1975-01-01 00:00:00
abstract::The renal alterations induced by alkali-treated protein and lysinoalanine are reviewed and their biological implications discussed. Alkali-treated proteins and lysinoalanine, an unusual amino acid formed in proteins during alkali treatment, have been shown to produce a renal alteration characterized by nuclear and cyt...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4757-9113-6_2
更新日期:1977-01-01 00:00:00
abstract::Our understanding of breast cancer and its therapeutic approach has improved greatly due to the advancement of molecular biology in recent years. Clinically, breast cancers are characterized into three basic types based on their immunohistochemical properties. They are triple-negative breast cancer, estrogen receptor ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-981-10-6020-5_4
更新日期:2017-01-01 00:00:00
abstract::Recruitment of neutrophils to sites of inflammation is now believed to occur through an initial rolling interaction at the luminal surface of activated endothelium and is mediated by a class of mammalian lectins referred to as the selectins. Selectins recognize carbohydrate determinants on co-receptors. It is generall...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-1885-3_31
更新日期:1995-01-01 00:00:00
abstract::Chronic cough is a common medical problem. The aim of the study was to analyze chronic cough causes in non-smoking patients and to search for demographic factors associated with different cough reasons. The etiology of cough was determined by medical history, diagnostic tests and response to specific treatment. Patien...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/5584_2015_153
更新日期:2015-01-01 00:00:00
abstract::Post-natal skeletal muscle is a highly plastic tissue that has the capacity to regenerate rapidly following injury, and to undergo significant modification in tissue mass (i.e. atrophy/hypertrophy) in response to global metabolic changes. These processes are reliant largely on soluble factors that directly modulate mu...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-27511-6_7
更新日期:2016-01-01 00:00:00
abstract::Immunotherapy entails the treatment of disease by modulation of the immune system. As detailed in the previous chapters, the different modes of achieving immune modulation are many, including the use of small/large molecules, cellular therapy, and radiation. Oncolytic viruses that can specifically attack, replicate wi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-94-017-7555-7_5
更新日期:2016-01-01 00:00:00
abstract::This chapter discusses the emerging field of vector-mediated antibody gene transfer as an alternative vaccine for infectious disease, with a specific focus on HIV. However, this methodology need not be confined to HIV-1; the general strategy of vector-mediated antibody gene transfer can be applied to other difficult v...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4939-2432-5_8
更新日期:2015-01-01 00:00:00
abstract::By use of an optical system, with which the beam of the first-order diffraction line of He-Ne laser light from a single frog skeletal muscle fiber was split by the wedge-shaped mirror to be focused on two photodiodes ( Haugen & Sten - Knudsen , 1976), small changes in sarcomere length (less than 1 A) could be recorded...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-4703-3_58
更新日期:1984-01-01 00:00:00
abstract::The propensity of prostate cancer cells to seed the skeleton and then progress into clinically relevant metastatic tumors is widely recognized and a major cause of morbidity and mortality for patients. The natural history of prostate adenocarcinoma most frequently begins with a tumor diagnosed at a localized stage, wh...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-32656-2_9
更新日期:2019-01-01 00:00:00
abstract::Recent progress in phenotyping of human dendritic cells (DCs) has allowed a closer alignment of the classification and functions of murine and human dendritic cell subsets. Marked differences in the functions of these human DC subsets and their response to HIV infection have become apparent, relevant to HIV pathogenes...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4614-4433-6_1
更新日期:2013-01-01 00:00:00
abstract::Aminopeptidase N (APN)/CD13 is a membrane-bound surface ectopeptidase with a ubiquitous distribution. In hematopoiesis, APN/CD13 is expressed on stem cells and during most developmental stages of myeloid cells. Because APN/CD13 has been implicated in the trimming on the cell surface of peptides that protrude out of MH...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/0-306-46826-3_5
更新日期:2000-01-01 00:00:00
abstract::Previously, we demonstrated that hypoxic pulmonary vasoconstriction (HPV) of intra-acinar arteries (IAA) requires mitochondrial complex II (= succinate dehydrogenase, SDH) activity (citeauthor ch41:paddenberg2006, Respir Res, 7:93, citeyear ch41:paddenberg2006). Interestingly, SDH subunits A and B have recently been d...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-90-481-2259-2_41
更新日期:2009-01-01 00:00:00
abstract::213 paired tcpO2/paO2-data and 186 paired tcSO2/SaO2-data measured in 25 newborns (10 term, 15 prematures) were compared. The correlation coefficient for tcpO2/paO2 was 0.796, for tcSO2/SaO2 0.944. Sensitivity for discriminating between normo- and hypoxemia (paO2 less than 50 torr) was 82% for the tcpO2- and 88% for t...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4613-1927-6_30
更新日期:1987-01-01 00:00:00
abstract::Many aspects of the structure of chymosin are quite unique even though structure comparisons indicate a high degree of structural homology with other eukaryotic aspartic proteinases. The structural homology is shown to be directly related to the sequence homology which varies from 30 to 60%. The recent structures of p...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-6012-4_3
更新日期:1991-01-01 00:00:00