Abstract：
:Polythiol binding of metal ions plays crucial role in the proper functioning of cysteine-rich proteins that are responsible for metal homeostasis and defending processes against metal toxicity (including heavy metals detoxification). The coordination properties of cysteine residues involved in specific sequencional patterns in proteins (like those present in e.g. metallothioneins) are interesting not only from a chemical point of view but may also lead to a better understanding of the purpose and allocation of metal ions in various biomolecules. In this study, the interaction of Zn2+, Cd2+ and Ni2+ ions with four peptides containing cysteine triplet motif were studied by potentiometric and spectroscopic methods. The main goal of this research was to answer the question how effectively three thiols, each being next to other, are able to bind single metal ion. Two of peptides contain additional, fourth cysteine residue, separated from triplet by two and three other amino acid residues. As results show, all three cysteine residues in the CCC motif are able to participate in the coordination of the metal ion (Cd2+, Zn2+). Except cysteine thiol groups, amide nitrogen atoms are also involved in the coordination of Ni2+.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Krzywoszyńska K,Swiątek-Kozłowska J,Potocki S,Ostrowska M,Kozłowski Hdoi
10.1016/j.jinorgbio.2019.110957subject
Has Abstractpub_date
2020-03-01 00:00:00pages
110957eissn
0162-0134issn
1873-3344pii
S0162-0134(19)30653-1journal_volume
204pub_type
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pub_type： 杂志文章,评审
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journal_title：Journal of inorganic biochemistry
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pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1989-05-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章,评审
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更新日期：2020-02-01 00:00:00
abstract：:[PtMe3(Me2CO)3]BF4 (1) reacts in acetone with 1,2,3,4-tetraacetyl-beta-D-glucopyranose (C2), pentaacetyl-alpha-D-glucopyranose (C3), pentaacetyl-beta-D-mannopyranose (C4) and pentaacetyl-beta-D-galactopyranose (C5) to give trimethyl(carbohydrate)platinum tetrafluoroborate complexes [PtMe3L]BF4 (2-5) (2, L=C2; 3, L=C3;...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/s0162-0134(00)00088-x
更新日期：2000-07-15 00:00:00
