Abstract:
BACKGROUND:Gene expression profiling of the salivary supernatant is emerging as a new and important source of real-time, systemic, biological information. However, existing technologies prevent RNA extraction of small quantities found in neonatal salivary supernatant. OBJECTIVE:The aim of this study was to develop techniques to enhance extraction of cell-free RNA from neonatal salivary supernatant. METHODS:Two saliva samples (10-100 μl) were serially collected from newborns (36-41 weeks' gestation) (n = 13) and stabilized. Total RNA was extracted from salivary supernatant with the use of two modified extraction techniques: Qiagen RNAprotect® Saliva Mini Kit (method 1) and the QIAamp Viral RNA Mini Kit (method 2). Quantitative RT-PCR amplification for GAPDH was performed on extracted salivary samples. Statistical analyses were performed on mean threshold cycle (Ct) levels to compare RNA yield from each protocol. Paired microarray analyses were made between neonatal whole saliva and supernatant (n = 3) to discern gene expression differences between these biolayers. RESULTS:mRNA was successfully extracted and amplified from all salivary supernatant samples. Extraction with method 2 yielded more RNA than with method 1 (p = 0.008). There was a 7.5% discordance between paired gene expression analyses for whole saliva and supernatant. Genes that were statistically significantly upregulated in supernatant highlighted 16 distinct biological functions not seen in whole saliva. Conversely, only two biological functions were unique to whole saliva. CONCLUSION:Neonatal cell-free salivary supernatant mRNA may be readily extracted and utilized on downstream applications. These technical enhancements allow for further exploration of the diagnostic potential of the neonatal salivary transcriptome.
journal_name
Neonatologyjournal_title
Neonatologyauthors
Dietz JA,Johnson KL,Wick HC,Bianchi DW,Maron JLdoi
10.1159/000328026subject
Has Abstractpub_date
2012-01-01 00:00:00pages
55-60issue
1eissn
1661-7800issn
1661-7819pii
000328026journal_volume
101pub_type
杂志文章相关文献
Neonatology文献大全abstract:AIM:Melatonin is a potent free radical scavenger and an indirect antioxidant. Knowledge about the behavior of melatonin secretion in the early neonatal period, which may relate to its properties at a vital stage during very high antioxidant demands, is limited. PATIENTS AND METHODS:We studied 35 newborns admitted to t...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000102957
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Perinatal hypoxic-ischemic brain damage is a major cause of acute mortality and chronic neurological morbidity in infants and children. Oxidative stress due to free radical formation and the initiation of abnormal oxidative reactions appears to play a key role. Docosahexanoic acid (DHA), a main component of ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000454982
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Doxapram has been advocated as a treatment for persistent apnea of prematurity (AOP). OBJECTIVE:To evaluate the effect of doxapram on long-term neurodevelopmental outcome in preterm infants as its safety still needs to be established. METHODS:From a retrospective cohort of preterm infants with a gestationa...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000444006
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Retinopathy of prematurity (ROP) is a sight-threatening disease affecting extremely preterm infants. The introduction of new ROP screening surveillance systems, with higher sensitivity and specificity than established ROP screening guidelines, has the potential to reduce the number of stressful eye examinati...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000435770
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:We hypothesized that color Doppler-measured cerebral blood flow velocity (CD-CBFV) as measured in the three feeding arteries of the brain can be used as an estimator of global cerebral blood flow. PATIENTS AND METHODS:CD-CBFV was determined as soon as possible after determination of phase-contrast magnetic ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000342336
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Congenital diaphragmatic hernia (CDH) is an abnormal development of the diaphragm leading to high neonatal mortality and morbidity. Beyond the neonatal period, prospective data on overall long-term outcome in CDH survivors is scarce. In particular, for those treated with fetoscopic endoluminal tracheal occlu...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000479145
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Around 10% of newborn infants require assistance during transition after birth. Heart rate (HR) is the most important clinical indicator to evaluate the clinical status of a newborn. AIM:Our study aimed to review all established and novel methods to detect HR in babies giving special consideration to non-in...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000499675
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Lipid metabolism is vital to fetal development and cardiometabolic health and the final weeks of gestation are known to be a time of intense metabolic activity. New techniques such as lipidomics allow investigation of a complex lipidomic profile in infants. OBJECTIVES:This research aimed to (1) describe var...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000487506
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Pharmacological closure of patent ductus arteriosus (PDA) is commonly achieved by intravenous (IV) administration of ibuprofen or indomethacin. Occasionally, oral ibuprofen is used for PDA treatment although its efficacy and safety are unclear. OBJECTIVES:To systematically review randomized and quasi-random...
journal_title:Neonatology
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000335332
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Small for gestational age (SGA) neonates are at increased risk of mortality and morbidity, including necrotizing enterocolitis (NEC), but detailed information on the incidence and risk factors of NEC in SGA neonates is lacking. OBJECTIVE:This study aims to estimate the incidence of NEC in a large cohort of ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000356033
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Hypoxic-ischaemic encephalopathy is a major cause of neurologic impairment and mortality in neonates. Early knowledge of brain injury is important to guide therapeutic decisions and reliably inform the parents. Increased secretoneurin levels have been detected in adult patients suffering from brain injury an...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000444245
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Meconium displaces surfactant from the alveolar surface and inhibits its function. The development of active synthetic surfactants is complicated, especially to synthesize the hydrophobic surfactant proteins SP-B and SP-C. A synthetic surfactant, CHF5633 containing SP-B and SP-C analogs, has been designed to...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000356065
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Cardiac troponin T (cTnT) has been proposed as specific biochemical marker for myocardial infarction in adults. Cardiac function in neonates could be influenced by the severity of respiratory distress and its ventilatory management. OBJECTIVES:To establish a normal range of cTnT in healthy neonates, compare...
journal_title:Neonatology
pub_type: 临床试验,杂志文章
doi:10.1159/000100964
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The postnatal heart responds to biomechanical stress with hypertrophy. The fetal heart may also undergo hyperplasia, but the percentage of mitotically active cardiomyocytes decreases throughout gestation. The signaling pathways controlling growth and proliferation in the fetal heart are poorly understood. O...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000107729
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Genetic processes underlying fetal lung development and maturation are incompletely understood. Better knowledge of these processes would provide insights into the causes of lung malformations and prevention of respiratory distress syndrome and the potential adverse effects of glucocorticoids. Hox genes are ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000201739
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Substantial health care resources are expended on standardised formal neonatal resuscitation training (SFNRT) programmes, but their effectiveness has not been proven. OBJECTIVES:To determine whether SFNRT programmes reduce neonatal mortality and morbidity, improve acquisition and retention of knowledge and ...
journal_title:Neonatology
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000443875
更新日期:2016-01-01 00:00:00
abstract::The aim of this article is to educate neonatal caregivers about metagenomics. This scientific field uses novel and ever changing molecular methods to identify how infants become colonized with microbes after birth. Publications using metagenomics appear infrequently in the neonatal literature because clinicians are un...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000354944
更新日期:2014-01-01 00:00:00
abstract::Despite recent advances in the perinatal management of neonatal respiratory distress syndrome (RDS), controversies still exist. We report the updated recommendations of a European panel of expert neonatologists who had developed consensus guidelines after critical examination of the most up-to-date evidence in 2007. T...
journal_title:Neonatology
pub_type: 杂志文章,实务指引
doi:10.1159/000297773
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:Carriers of Staphylococcus aureus strains can be the source of epidemic infection for patients. OBJECTIVES:A molecular epidemiological analysis of an impetigo bullosa outbreak in a neonatal ward was performed in order to determine a potential source of the infection and possible routes of subsequent spreadi...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000204961
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Despite increasing survival in the smallest preterm infants, the incidence of chronic lung disease has not decreased. Research into ventilatory strategies has concentrated on minimising barotrauma, volutrauma and atelectotrauma, but little attention has been paid to the role of bias gas flow rates and the po...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000220765
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:In the absence of universal screening for congenital cytomegalovirus (cCMV) infection, the aim of this study was to assess the outcomes of a targeted screening protocol based on maternal and neonatal risk indicators. METHODS:The medical records of 2,623 neonates born in our maternal hospital between June 20...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000512525
更新日期:2020-12-22 00:00:00
abstract:BACKGROUND:Targeted milrinone treatment for low left ventricular output (LVO) reduces the incidence of acute cardiorespiratory instability following ligation of patent ductus arteriosus (PDA) in preterm infants. Despite this, some infants continue to experience postoperative deterioration. Adrenal insufficiency related...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000350017
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Minimizing initial neonatal blood draws and their associated pain is important. The placenta has ample fetal blood that is otherwise discarded; obtaining admission laboratory evaluations from fetal umbilical venous blood (FUVB) may provide a suitable alternative. OBJECTIVE:We hypothesized that obtaining an ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000491993
更新日期:2019-01-01 00:00:00
abstract::There is mounting evidence that early continuous positive airway pressure (CPAP) from birth is feasible and safe even in very preterm infants. However, many infants will develop respiratory distress syndrome (RDS) and require surfactant treatment. Combining a noninvasive ventilation approach with a strategy for surfac...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000381122
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The causes of bronchopulmonary dysplasia (BPD) are multifactorial. Overdistension of the lung (volutrauma) is considered an important contribution. As an alternative to traditional pressure-limited ventilation (PLV), modern neonatal ventilators offer modes which can target a set tidal volume. OBJECTIVES:To ...
journal_title:Neonatology
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000326080
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:The identification of factors involved in the postnatal growth of preterm infants will help achieve growth similar to that of term infants. OBJECTIVES:As per protocol: to compare body composition in very preterm infants at term-corrected age (TCA) with that in term infants, and to explore relationships betw...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000450882
更新日期:2017-01-01 00:00:00
abstract::This paper describes a neonate with type II Gaucher disease. The phenotype was unusually severe with congenital ichthyosis, hepatosplenomegaly, muscular hypotonia, myoclonus and respiratory failure. Electron microscopy of the skin revealed lamellar body contents in the stratum corneum interstices, appearances consider...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000324116
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Streptococcus agalactiae (group B Streptococcus, GBS) is the most common cause of early neonatal infection, but restricting the diagnosis to culture-positive infants may underestimate the burden of GBS disease. Our objective was to determine whether maternal GBS colonization was associated with an increased ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000434716
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The incidence of adverse tracheal intubation-associated events (TIAEs) and associated patient, practice, and intubator characteristics in the neonatal intensive care unit (NICU) setting are unknown. OBJECTIVES:To determine the incidence of adverse TIAEs and to identify factors associated with TIAEs in the N...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000381252
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Higher patient-to-nurse ratios and nursing workload are associated with increased mortality in the adult intensive care unit (ICU). Most neonatal ICUs (NICUs) in the United Kingdom do not meet national staffing recommendations. The impact of staffing on outcomes in the NICU is unknown. OBJECTIVE:To determin...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000353458
更新日期:2013-01-01 00:00:00