D-galactose induces necroptotic cell death in neuroblastoma cell lines.

Abstract:

:D-Galactose (D-gal) can induce oxidative stress in non-cancer cells and result in cell damage by disturbing glucose metabolism. However, the effect of D-gal on cancer cells is yet to be explored. In this study, we investigated the toxicity of D-gal to malignant cells specifically neuroblastoma cells. As the results, high concentrations of D-gal had significant toxicity to cancer cells, whereas the same concentrations of glucose had no; the viability loss via D-gal treatment was prominent to malignant cells (Neuro2a, SH-SY5Y, PC-3, and HepG2) comparing to non-malignant cells (NIH3T3 and LO(2)). Differing from the apoptosis induced by H(2) O(2), D-gal damaged cells showed the characters of necrotic cell death, such as trypan blue-tangible and early phase LDH leakage. Further experiments displayed that the toxic effect of D-gal can be alleviated by necroptosis inhibitor Necrostatin (Nec-1) and autophagy inhibitor 3-methyladenine (3-MA) but not by caspase inhibitor z-VAD-fmk. D-Gal treatment can transcriptionally up-regulate the genes relevant to necroptosis (Bmf, Bnip3) and autophagy (Atg5, TIGAR) but not the genes related to apoptosis (Caspase3, Bax, and p53). D-Gal did not activate Caspase-3, but prompted puncta-like GFP-LC3 distribution, an indicator for activated autophagy. The involvement of aldose reductase (AR)-mediated polyol pathway was proved because the inhibitor of AR can attenuate the toxicity of D-gal and D-gal treatment elevates the expression of AR. This study demonstrates for the first time that D-gal can induce non-apoptotic but necroptotic cell death in neuroblastoma cells and provides a new clue for developing the strategy against apoptosis-resistant cancers.

journal_name

J Cell Biochem

authors

Li N,He Y,Wang L,Mo C,Zhang J,Zhang W,Li J,Liao Z,Tang X,Xiao H

doi

10.1002/jcb.23314

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

3834-44

issue

12

eissn

0730-2312

issn

1097-4644

journal_volume

112

pub_type

杂志文章
  • Light and electron microscopic immunocytochemical localization of two major proteins in garlic bulb.

    abstract::Garlic is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, antidiabetic, and anticoagulant. Two major proteins of 40 KD and 14 KD constituting approximately 96% of total garlic proteins have been recently purified at our Institute. This immunocytochemic...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240580411

    authors: Wen GY,Mato A,Wisniewski HM,Malik MN,Jenkins EC,Sheikh AM,Kim KS

    更新日期:1995-08-01 00:00:00

  • Cell-cycle-dependent localization of human cytomegalovirus UL83 phosphoprotein in the nucleolus and modulation of viral gene expression in human embryo fibroblasts in vitro.

    abstract::The nucleolus is a multifunctional nuclear compartment widely known to be involved in several cellular processes, including mRNA maturation and shuttling to cytoplasmic sites, control of the cell cycle, cell proliferation, and apoptosis; thus, it is logical that many viruses, including herpesvirus, target the nucleolu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22928

    authors: Arcangeletti MC,Rodighiero I,Mirandola P,De Conto F,Covan S,Germini D,Razin S,Dettori G,Chezzi C

    更新日期:2011-01-01 00:00:00

  • Evidence for inhibition of MyEF-2 binding to MBP promoter by MEF-1/Pur alpha.

    abstract::Myelin basic protein (MBP) is a major component of the myelin sheath whose production is developmentally controlled during myelinogenesis. Earlier studies have indicated that programmed expression of the MBP gene is regulated at the level of transcription. Evidently, the MB1 regulatory motif located between nucleotide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19970915)66:4<524::aid-jcb

    authors: Muralidharan V,Tretiakova A,Steplewski A,Haas S,Amini S,Johnson E,Khalili K

    更新日期:1997-09-15 00:00:00

  • Matrix influence on the tumor cell stimulation of fibroblast collagenase production.

    abstract::Cocultures of rabbit fibroblasts and mouse B-16 melanoma cells produce increased levels of collagenase against type I collagen. This stimulatory effect was also found when fibroblasts were cultured in conditioned media from tumor cells. However, the level of the stimulatory factor in conditioned media was influenced b...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240280107

    authors: Biswas C

    更新日期:1985-01-01 00:00:00

  • Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy.

    abstract::Cells release exosomes to transfer various molecules to other cells. Exosomes are involved in a number of physiological and pathological processes. They are emerging great potential utility for diseases diagnosis and treatment recently. However, the internalization and intracellular trafficking of exosomes have not be...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22733

    authors: Tian T,Wang Y,Wang H,Zhu Z,Xiao Z

    更新日期:2010-10-01 00:00:00

  • Chrysophanol induces cell death and inhibits invasiveness via mitochondrial calcium overload in ovarian cancer cells.

    abstract::Chrysophanol is a phytochemical typically extracted from rhubarb. Similar to other extracts from rhubarb, chrysophanol possesses anticancer activity against diverse cancerous cells. However, the apoptotic effects of chrysophanol in ovarian cancer remain unknown. In the current study, we examined the antitumorigenic ac...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27363

    authors: Lim W,An Y,Yang C,Bazer FW,Song G

    更新日期:2018-12-01 00:00:00

  • Modulation of O-GlcNAc glycosylation during Xenopus oocyte maturation.

    abstract::O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a post-translational modification, which is believed antagonises phosphorylation. We have studied the O-GlcNAc level during Xenopus oocyte meiotic resumption, taking advantage of the high synchrony of this model which is dependent upon a burst of phosphorylation...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20242

    authors: Lefebvre T,Baert F,Bodart JF,Flament S,Michalski JC,Vilain JP

    更新日期:2004-11-15 00:00:00

  • Heterogeneous induction of an invasive phenotype in prostate cancer cells by coculturing with patient-derived fibroblasts.

    abstract::Prostate cancer (PCa) cells frequently invade the surrounding stroma, leading to heterogeneous formation of structural atypia. The surrounding stroma contains multiple functionally diverse populations of fibroblasts that trigger numerous changes in PCa cells including motility. Thus, we hypothesized that direct or ind...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29893

    authors: Ishii K,Nakagawa Y,Matsuda C,Katoh D,Ichishi M,Shirai T,Hirokawa Y,Fujiwara M,Sugimura Y,Watanabe M

    更新日期:2021-01-22 00:00:00

  • Upregulation of long noncoding RNA TUG1 contributes to the development of laryngocarcinoma by targeting miR-145-5p/ROCK1 axis.

    abstract::Laryngocarcinoma is the most common head and neck cancer and has a high incidence and mortality, causing about 83 000 deaths per year worldwide. Our research aimed to investigate the possible role of long noncoding RNA (lncRNA) taurine upregulated gene 1 (TUG1) in laryngocarcinoma development. The messenger RNA (mRNA)...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28614

    authors: Zhuang S,Liu F,Wu P

    更新日期:2019-08-01 00:00:00

  • miR-21 downregulates the tumor suppressor P12 CDK2AP1 and stimulates cell proliferation and invasion.

    abstract::The present study was undertaken to investigate the regulation of P12(CDK2AP1) by miRNAs. A conserved target site for miR-21 within the CDK2AP1-3'-UTR at nt 349-370 was predicted by bioinformatics software and an inverse correlation of miR-21 and CDK2AP1 protein was observed. Highly specific amplification and quantifi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22995

    authors: Zheng J,Xue H,Wang T,Jiang Y,Liu B,Li J,Liu Y,Wang W,Zhang B,Sun M

    更新日期:2011-03-01 00:00:00

  • Identification of novel inhibitors against UDP-galactopyranose mutase to combat leishmaniasis.

    abstract::Leishmania, a protozoan parasite that causes leishmaniasis, affects 1-2 million people every year worldwide. Leishmaniasis is a vector born disease and characterized by a diverse group of clinical syndromes. Current treatment is limited because of drug resistance, high cost, poor safety, and low efficacy. The urgent n...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26433

    authors: Kashif M,Tabrez S,Husein A,Arish M,Kalaiarasan P,Manna PP,Subbarao N,Akhter Y,Rub A

    更新日期:2018-03-01 00:00:00

  • Characterization of the protective effects of melatonin and related indoles against alpha-naphthylisothiocyanate-induced liver injury in rats.

    abstract::The protective effect of melatonin, 6-hydroxymelatonin and N-acetylserotonin against alpha-naphthylisothiocyanate (ANIT)-induced liver injury was investigated and compared in rats injected once with the hepatotoxicant (75 mg/kg body weight). In rats injected with ANIT alone, liver injury with cholestasis developed wit...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/1097-4644(20010315)80:4<461::aid-jcb1000>3

    authors: Calvo JR,Reiter RJ,García JJ,Ortiz GG,Tan DX,Karbownik M

    更新日期:2001-01-01 00:00:00

  • An intranuclear frame for chromatin compartmentalization and higher-order folding.

    abstract::Recent ultrastructural, immunoelectron, and confocal microscopy observations done in our laboratory [Barboro et al. [2002] Exp. Cell. Res. 279:202-218] have confirmed that lamins and the nuclear mitotic apparatus protein (NuMA) are localized inside the interphase nucleus in a polymerized form. This provided evidence o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10378

    authors: Barboro P,D'Arrigo C,Mormino M,Coradeghini R,Parodi S,Patrone E,Balbi C

    更新日期:2003-01-01 00:00:00

  • Transcriptional regulation of the lysozyme gene in airway gland serous cells.

    abstract::Lysozyme is expressed in serous, but not mucous, cells of the tracheobronchial glands and thereby constitutes a marker of the serous cell lineage in these glands. To identify DNA regulatory elements and transcription factors mediating the commitment of progenitor cells to the serous cell lineage, we have characterized...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4644(19960601)61:3%3C350::AID-J

    authors: Kai H,Takeuchi K,Ohmori H,Li JD,Gallup M,Basbaum C

    更新日期:1996-06-01 00:00:00

  • Integrin alpha V beta 3 targeted dendrimer-rapamycin conjugate reduces fibroblast-mediated prostate tumor progression and metastasis.

    abstract::Therapeutic strategies targeting both cancer cells and associated cells in the tumor microenvironment offer significant promise in cancer therapy. We previously reported that generation 5 (G5) dendrimers conjugated with cyclic-RGD peptides target cells expressing integrin alpha V beta 3. In this study, we report a nov...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26727

    authors: Hill EE,Kim JK,Jung Y,Neeley CK,Pienta KJ,Taichman RS,Nor JE,Baker JR Jr,Krebsbach PH

    更新日期:2018-11-01 00:00:00

  • cAMP-dependent protein kinase modulates expression and subcellular localization of Dolichos biflorus agglutinin binding sites in renal epithelial cells.

    abstract::Previous studies demonstrated that, upon attaining confluence, a clone of the renal epithelial cell, LLC-PK1, expressed progressively binding sites for the lectin Dolichos biflorus agglutinin (DBA) at the apical cell surface. Activation of cAMP-dependent protein kinase enhanced surface expression dramatically. The goa...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240520412

    authors: Amsler K

    更新日期:1993-08-01 00:00:00

  • LncRNA NEAT1 promotes hypoxia-induced renal tubular epithelial apoptosis through downregulating miR-27a-3p.

    abstract::Acute kidney injury (AKI) is a common kidney disorder that affects public health and the incidence of AKI. Sepsis, acute ischemia or hypoxia is the main reason for the occurrence of AKI. Recently, noncoding RNA that include microRNA and long noncoding RNA (lncRNAs) were reported to play important roles in AKI as well ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28909

    authors: Jiang X,Li D,Shen W,Shen X,Liu Y

    更新日期:2019-09-01 00:00:00

  • Neutrophil apoptosis: a target for enhancing the resolution of inflammation.

    abstract::Neutrophils are essential for host defense and their programmed cell death and removal are critical for the optimal expression as well as for efficient resolution of inflammation. Delayed neutrophil apoptosis or impaired clearance of apoptotic neutrophils by macrophages contributes to the progression of chronic inflam...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.22351

    authors: Filep JG,El Kebir D

    更新日期:2009-12-01 00:00:00

  • Inhibition of prolidase activity by nickel causes decreased growth of proline auxotrophic CHO cells.

    abstract::Occupational exposure to nickel has been epidemiologically linked to increased cancer risk in the respiratory tract. Nickel-induced cell transformation is associated with both genotoxic and epigenetic mechanisms that are poorly understood. Prolidase [E.C.3.4.13.9] is a cytosolic Mn(II)-activated metalloproteinase that...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20384

    authors: Miltyk W,Surazynski A,Kasprzak KS,Fivash MJ Jr,Buzard GS,Phang JM

    更新日期:2005-04-15 00:00:00

  • Role of cell signalling involved in induction of apoptosis by benzo[a]pyrene and cyclopenta[c,d]pyrene in Hepa1c1c7 cells.

    abstract::The reactive metabolites of benzo[a]pyrene (B[a]P) and cyclopenta[c,d]pyrene (CPP) induced an accumulation/phosphorylation of p53 in Hepa1c1c7 cells, whereas inhibition of p53 reduced the apoptosis. Judged by the inhibiting effect of wortmannin, phosphatidyl-inositol-3 (PI-3) kinases such as DNA-dependent protein kina...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20251

    authors: Solhaug A,Refsnes M,Holme JA

    更新日期:2004-12-15 00:00:00

  • cAMP-dependent protein kinase inhibits the mitogenic action of vascular endothelial growth factor and fibroblast growth factor in capillary endothelial cells by blocking Raf activation.

    abstract::Proliferation of endothelial cells is regulated by angiogenic and antiangiogenic factors whose actions are mediated by complex interactions of multiple signaling pathways. Both vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) stimulate cell proliferation and activate the mitogen-acti...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: D'Angelo G,Lee H,Weiner RI

    更新日期:1997-12-01 00:00:00

  • Butyrate stimulates ApoA-IV-containing lipoprotein secretion in differentiated Caco-2 cells: role in cholesterol efflux.

    abstract::The aim of this study was to determine: (1) whether the Short Chain Fatty Acids (SCFA) Acetate, Propionate, and Butyrate enhance the synthesis and secretion of intestinal apolipoprotein A-IV-containing lipoproteins and (2) if so, whether these particles are able to promote cholesterol efflux in vitro. For this purpose...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1221

    authors: Nazih H,Nazih-Sanderson F,Krempf M,Michel Huvelin J,Mercier S,Marie Bard J

    更新日期:2001-08-01 00:00:00

  • Characterization of LipN (Rv2970c) of Mycobacterium Tuberculosis H37Rv and its Probable Role in Xenobiotic Degradation.

    abstract::LipN (Rv2970c) belongs to the Lip family of M. tuberculosis H37Rv and is homologous to the human Hormone Sensitive Lipase. The enzyme demonstrated preference for short carbon chain substrates with optimal activity at 45°C/pH 8.0 and stability between pH 6.0-9.0. The specific activity of the enzyme was 217 U/mg protein...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25285

    authors: Jadeja D,Dogra N,Arya S,Singh G,Singh G,Kaur J

    更新日期:2016-02-01 00:00:00

  • Basic fibroblast growth factor decreases type V/XI collagen expression in cultured bovine aortic smooth muscle cells.

    abstract::Vascular smooth muscle cells (SMCs), the major cellular constituent of an artery, synthesize the bulk of fibrillar collagens, including type V/XI, which regulates heterotypic collagen fibril assembly. Basic fibroblast growth factor (bFGF) is a heparin-binding polypeptide growth factor that has been implicated in impor...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19980201)68:2<247::aid-jcb

    authors: Kypreos KE,Sonenshein GE

    更新日期:1998-02-01 00:00:00

  • Gene array identification of osteoclast genes: differential inhibition of osteoclastogenesis by cyclosporin A and granulocyte macrophage colony stimulating factor.

    abstract::Treatment of adherent peripheral blood mononuclear cells (PBMCs) with macrophage colony stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL) stimulates the formation of multinucleate osteoclast-like cells. Treatment with M-CSF alone results in the formation of macrophage-like cells. Through th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10780

    authors: Day CJ,Kim MS,Stephens SR,Simcock WE,Aitken CJ,Nicholson GC,Morrison NA

    更新日期:2004-02-01 00:00:00

  • Localization and regulation of phospholipase D2 by ARF6.

    abstract::Phospholipase D (PLD) and ADP-ribosylation factor 6 (ARF6) have been implicated in vesicular trafficking and rearrangement of the actin cytoskeleton. We have explored the co-localization of rat PLD1b and rat PLD2 with wild type and mutant forms of ARF6 in HeLa cells and studied their activation by ARF6 and the role of...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20351

    authors: Hiroyama M,Exton JH

    更新日期:2005-05-01 00:00:00

  • Transcriptional regulation of Runx2 by HSP90 controls osteosarcoma apoptosis via the AKT/GSK-3β/β-catenin signaling.

    abstract::Osteosarcoma (OS) is the most malignant primary bone tumor in children and adolescents with limited treatment options and poor prognosis. Recently, aberrant expression of Runx2 has been found in OS, thereby contributing to the development, and progression of OS. However, the upstream signaling molecules that regulate ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26260

    authors: Liang GH,Liu N,He MT,Yang J,Liang ZJ,Gao XJ,Rahhal AH,He QY,Zhang HT,Zha ZG

    更新日期:2018-01-01 00:00:00

  • Expression Profiles Analysis and Functional Characterization of MicroRNA-660 in Skeletal Muscle Differentiation.

    abstract::MicroRNA are a series of small non-coding RNAs that have emerged as critical regulators of skeletal muscle development. Here, we concentrated on the function of miR-660 during bovine skeletal myogenesis from our previous high-throughput sequencing results, then analyzed its expression profiles and characterized relate...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25901

    authors: Yue B,Wu J,Wang Y,Zhang C,Fang X,Chen H

    更新日期:2017-08-01 00:00:00

  • Free Fatty Acids Induce Autophagy and LOX-1 Upregulation in Cultured Aortic Vascular Smooth Muscle Cells.

    abstract::Elevation of free fatty acids (FFAs) is known to affect microvascular function and contribute to obesity-associated insulin resistance, hypertension, and microangiopathy. Proliferative and synthetic vascular smooth muscle cells (VSMCs) increase intimal thickness and destabilize atheromatous plaques. This study aimed t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25784

    authors: Cheng CI,Lee YH,Chen PH,Lin YC,Chou MH,Kao YH

    更新日期:2017-05-01 00:00:00

  • Endoplasmic reticulum stress triggers an acute proteasome-dependent degradation of ATF6.

    abstract::ATF6, a 670 amino acid endoplasmic reticulum (ER) transmembrane glycoprotein with the electrophoretic mobility of a 90 kDa protein, is a key transcriptional activator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis when the cells are subjected to a variety of environ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20118

    authors: Hong M,Li M,Mao C,Lee AS

    更新日期:2004-07-01 00:00:00