Abstract:
:The purpose of this study was to evaluate the effect of direct administration of nerve growth factor (NGF) into an epineural conduit across a short nerve gap (10 mm) in a rabbit sciatic nerve model. The animals were divided into two groups. In group 1, n = 6, a 10-mm defect was created in the sciatic nerve and bridged with an epineural flap. A dose of 1 μg of NGF was locally administered daily for the first 21 days. NGF administration was made inside the epineural flap using a silicone reservoir connected to a silicone tube. In group 2, n = 6, the 10-mm defect was bridged with a nerve graft. This group did not receive any further treatment. At 13 weeks, all animals, before euthanasia, underwent electromyography (EMG) studies and then specimen sent for histology morphometric analysis. NGF administration ensured a significantly increased average number of myelinated axons per μm(2) (P = 0.028) and promoted fiber maturation (P = 0.031) and better EMG results (P = 0.046 for latency P = 0.048 for amplitude), compared with the control group. Although nerve grafts remain the gold standard for peripheral nerve repair, NGF-treated epineural conduits represent a good alternative, particularly when an unfavorable environment for nerve grafts is present.
journal_name
Microsurgeryjournal_title
Microsurgeryauthors
Barmpitsioti A,Konofaos P,Ignatiadis I,Papalois A,Zoubos AB,Soucacos PNdoi
10.1002/micr.20925subject
Has Abstractpub_date
2011-10-01 00:00:00pages
545-50issue
7eissn
0738-1085issn
1098-2752journal_volume
31pub_type
杂志文章相关文献
MICROSURGERY文献大全abstract:OBJECTIVES:Plate extrusions after free tissue transfer for mandibular reconstruction can be problematic and generally require revision surgery. Our objective was to assess the predictors of plate extrusion and compare outcomes between fibular free flaps (FFF), lateral border scapular flaps (LBSF), and scapular tip free...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.30349
更新日期:2018-09-01 00:00:00
abstract::Several microsurgical techniques have been described for the treatment of osteonecrosis of the talus (ONT). Recently reported in children, vascularized periosteal grafts showed promising revascularizing properties. We report a novel technique using a pedicled periosteal graft from the first metatarsal bone to treat st...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.22045
更新日期:2013-01-01 00:00:00
abstract::Two groups of consecutive patients treated with latissimus dorsi free myocutaneous flaps to cover lower-extremity soft-tissue defects were compared for postoperative vascular occlusion. In the first group of 13 patients (group I), there were five (38%) venous occlusions and no arterial occlusions. The second group of ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920120414
更新日期:1991-01-01 00:00:00
abstract::The authors report their experience of the application of microsurgical techniques in total and subtotal thyroidectomy operations from January 1984-January 1997. Four hundred and eighty-six patients with thyroid diseases were operated upon with both traditional and microsurgical techniques. The comparison of results s...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/(sici)1098-2752(1998)18:8<460::aid-micr6>3
更新日期:1998-01-01 00:00:00
abstract::Improved microsurgical techniques for kidney transplantation in the mouse are described. Left renal transplantation is performed with end-to-side anastomoses of the donor renal vein to the inferior vena cava and the donor aortic cuff to the aorta. Urinary tract reconstruction is accomplished by suturing the donor uret...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920160212
更新日期:1995-01-01 00:00:00
abstract::Between July 1986 and July 1994, we studied 125 total finger amputations due to crush and avulsion type injuries at the Izmir Hand and Microsurgery Hospital. Of the 125 fingers replanted, 88 were successful, for a general survival rate of 70.4%. In the period between 1986 and 1991, we performed end-to-end anastomoses ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920161107
更新日期:1995-01-01 00:00:00
abstract::In order to be able to objectively evaluate capillary perfusion of venous flaps, we created arterialised venous flaps, venous flow-through flaps, and unilateral pedicled venous island flaps in the epigastric vascular system of Wistar rats and determined their oxygen supply with two different methods. Interstitial pO2 ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/(sici)1098-2752(1998)18:5<324::aid-micr5>3
更新日期:1998-01-01 00:00:00
abstract::Monoclonal antibody directed against CD40 ligand prevents acute allograft rejection in several models of solid-organ transplantation. This study describes the use of CD40 ligand as antirejection therapy in a mouse peripheral nerve allograft model. C3H mice received 8-mm nerve isografts (n = 2) or nerve allografts from...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20028
更新日期:2004-01-01 00:00:00
abstract::We have developed a chitosan/polyglycolic acid (PGA) artificial nerve graft which was previously used for bridge implantation of dog sciatic nerves across 30-mm long defects. Here we describe a clinical trial of this graft for repairing a 35-mm-long median nerve defect at elbow of a human patient. During the 3-year fo...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20488
更新日期:2008-01-01 00:00:00
abstract::We employed a forearm flap that had been thinned through primary defatting for nasal covering in three cases to reduce the need for secondary revision. Partial flap loss did occur in one case due to subcutaneous vascular plexus injury, but acceptable results were obtained in the remaining two cases. Donor site concave...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2752(1996)17:4<184::AID-MICR4>3
更新日期:1996-01-01 00:00:00
abstract::This is an experimental study comparing the suturing time and patency rate of a spiral interrupted suturing technique to those of conventional interrupted and continuous suturing technique in end-to-end as well as end-to-side anastomosis. The spiral interrupted suturing technique requires less time for either end-to-e...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920070205
更新日期:1986-01-01 00:00:00
abstract::Vibration syndrome, a clinical condition arising from chronic use of vibrating tools, is associated with a spectrum of neurovascular symptoms. To date, only its vascular pathology has been extensively studied; we sought to determine what direct neurologic injury, if any, is caused by vibration. Hindlimbs of anesthetiz...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20081
更新日期:2005-01-01 00:00:00
abstract::The potential for hyperbaric oxygen therapy (HBO) to decrease the untoward effects of a secondary ischemic event was studied in the rat superficial epigastric flap model. The secondary venous ischemic flap was created by cross-clamping the vascular pedicles for 2 h. Twenty-four hours later, the flap was reelevated and...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.21723
更新日期:2002-01-01 00:00:00
abstract::In this paper, we report on the anatomical study of 34 cadaveric forearms with red latex injection and the clinical application of this study to 11 cases of microsurgical second dorsal metacarpal artery (SDMA) flaps. There were 8 cutaneous cases and 3 tenocutaneous cases using SDMA flaps for distal finger reconstructi...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20077
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:We describe a new dual neurorrhaphy method for a free abdominal-based flap and compare sensory recovery with this novel technique to that with conventional neurorrhaphy technique for breast reconstruction. METHODS:70 breast cancer patients underwent muscle sparing innervated transversal rectus abdominis myo...
journal_title:Microsurgery
pub_type: 临床试验,杂志文章
doi:10.1002/micr.30064
更新日期:2017-01-01 00:00:00
abstract::Reconstruction of the foot using microvascular flaps has been widely performed in the last 15 years but the choice of flap to repair some areas in the foot remains controversial. We present a series of 128 free flaps to the foot performed during the period of 1975-1990. One hundred and seventeen had a successful outco...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920150110
更新日期:1994-01-01 00:00:00
abstract::Studies of lymphatic and venous pressures of normal and lymphedematous rabbit ears reveal the end pressure of lymphatics to be higher than the venous pressure. Massage of the related rabbit ear increases the lymphatic pressures and can markedly raise the side and end pressures within the lymphatic wall. The venous pre...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920050108
更新日期:1984-01-01 00:00:00
abstract::By applying a novel biochemical technique using porcine tendon as the raw material with its antigen minimized, we developed an artificial biological ligament (ABL). We examined and tested its structure, mechanical properties, and biocompatibility and explored the feasibility of reconstructing the anterior cruciate lig...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20442
更新日期:2008-01-01 00:00:00
abstract::This study recognizes recent advances in the understanding of the anatomy and physiology of peripheral nerves at the cellular level. It has reproduced study conditions originally advocated by de Medinaceli and coworkers, with modifications. Eighty-four rats were divided into three groups. Group A underwent sciatic ner...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920100408
更新日期:1989-01-01 00:00:00
abstract::Perineal and posterior vaginal wall reconstruction following abdominoperineal and local cancer resection entails replacement of volume between the perineum and sacrum and restoration of a functional vagina. Ideal local reconstructive options include those which avoid functional muscle sacrifice, do not interfere with ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20663
更新日期:2009-01-01 00:00:00
abstract::Reconstructive surgery of the head and neck has particularly benefited from the rapid developments in microsurgery. Resection of tumours from the head and neck which are associated with serious physical and psychological problems, or which often require multiple reconstructive procedures, can now be performed as a sin...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920150711
更新日期:1994-01-01 00:00:00
abstract:INTRODUCTION:Reconstruction of the lower leg commonly requires a free tissue transfer after Gustillo grade IIIB-IIIC injuries and severe postoncological resections, where, free musculocutaneous flaps (MCF) are preferred for their size and robust blood supply. The anastomoses are performed at more proximal levels to kee...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20755
更新日期:2010-09-01 00:00:00
abstract::Recent supermicrosurgical techniques have developed the possibility for vascular anastomosis of smaller vessels and it is now safe and sound to perform precise anastomoses between lymphatics and venules. Reported here is the 2 years experience on supermicrosurgical lymphaticovenular anastomosis and/or lymphaticovenous...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20665
更新日期:2009-01-01 00:00:00
abstract::A study was designed to investigate the role of terminal probes as determinates of two-point discrimination in the normal hand. A Vernier caliper was modified to allow an exchange of probes with different diameters and with different terminal shapes. These terminal devices included flat, sharp, and rounded probe tips ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920100318
更新日期:1989-01-01 00:00:00
abstract::In clinical settings, combined heart and kidney transplantation results in a lower incidence of cardiac graft and renal graft rejection as compared to isolated heart and kidney transplantation. To study the phenomenon in an experimental setting, we developed a model of combined heart and kidney transplantation in mice...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.10111
更新日期:2003-01-01 00:00:00
abstract::This article studies the utilization of the end-to-side neurorrhaphy concept in the prevention and treatment of painful neuromas. A total of 20 rats were divided into 2 groups (10 rats per group). In group A, the tibial nerve was divided and left lying in the subcutaneous tissue. In group B, the cut ends of the tibial...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/(sici)1098-2752(2000)20:3<99::aid-micr1>3.
更新日期:2000-01-01 00:00:00
abstract::This study was designed to investigate the short-term formation and composition of thrombus occurring at sites of arterial intimal injury in rabbits. Anti-coagulants, platelet anti-aggregation agents, and fibrinolytic agents were evaluated for their influence upon the developing thrombus, utilizing scanning electron m...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920120413
更新日期:1991-01-01 00:00:00
abstract::It is generally known that low-intensity pulsed ultrasound (LIPUS) accelerates peripheral nerve tissue regeneration. However, the precise cellular mechanism involved is still unclear. The purpose of this study was to determine how the Schwann cells respond directly to LIPUS stimuli. Thus, we investigated the effect of...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20644
更新日期:2009-01-01 00:00:00
abstract::This study describes our experience using an improved technique for microsurgical sleeve anastomosis of arteries. Modifications of previously described techniques include utilization of a sidecut and placement of the two stitches within separate vertical planes. In 40 arteries, we attained a patency rate of 97.5%. His...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920120502
更新日期:1991-01-01 00:00:00
abstract::The purpose of this work was to report our initial experience with lymphaticovenular anastomoses (LVA), a controversial technique for lymphedema treatment. Although LVA technique was described many years ago, the procedure is not as widespread as it was supposed to be, taking into account the high impact that lymphede...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20980
更新日期:2012-05-01 00:00:00