Abstract:
:DNA molecules utilize adenine, thymine, cytosine, and guanine for coding genetic information. In addition to these four canonical nucleobases, DNA molecules also contain a variety of modified nucleobases that can control and regulate gene expression and chromosome structure. Elucidating the functions of DNA modifications relies on the sensitive detection, accurate quantification, and genome-wide mapping of these modifications in genomic DNA. The significant advances of techniques and methods in recent years have enabled the discovery and functional studies of a number of new modifications in DNA in both prokaryotes and eukaryotes. Mass spectrometry-based methods for analyzing DNA modifications have substantially advanced over the past decade, which has greatly stimulated the research of DNA epigenetic modifications. The emergence of next-generation sequencing technology provides complementary methodology to enable genome-wide mapping of modifications, which is very important to reveal the biological roles of DNA modifications. This perspective highlights the recent methodologies for the assessment of DNA modifications with focus on mass spectrometry and sequencing analytical strategies.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Yuan BFdoi
10.1021/acs.chemrestox.9b00372subject
Has Abstractpub_date
2020-03-16 00:00:00pages
695-708issue
3eissn
0893-228Xissn
1520-5010journal_volume
33pub_type
杂志文章abstract::The metabolism of genistein (4',5,7-trihydroxyisoflavone), a phytoestrogen derived from soy products, was investigated using rat and human liver microsomes and recombinant human cytochrome P450 enzymes. Metabolism of genistein by microsomes obtained from rats treated with pyridine, phenobarbital, beta-naphthoflavone, ...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx9802320
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx0600189
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx9501086
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.0c00013
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00021a016
更新日期:1991-05-01 00:00:00
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.8b00104
更新日期:2018-07-16 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.6b00222
更新日期:2016-10-17 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00015a002
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx050271t
更新日期:2006-01-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx000259v
更新日期:2001-05-01 00:00:00
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journal_title:Chemical research in toxicology
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doi:10.1021/tx2000015
更新日期:2011-05-16 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章,评审
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更新日期:2020-01-21 00:00:00
abstract::The neurotrophic factor pleiotrophin (PTN) is upregulated in different brain areas after the administration of different drugs of abuse, including psychostimulants. PTN has been shown to prevent cocaine-induced cytotoxicity in NG108-15 and PC12 cells. We previously demonstrated that specific phosphoproteins related to...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.5b00130
更新日期:2015-07-20 00:00:00
abstract::Xenobiotics may activate the estrogen receptor, resulting in alteration of normal endocrine functions in animals and humans. Consequently, this necessitates development of assay end points capable of identifying estrogenic xenobiotics. In the present study, we screened the potential estrogenicity of chemicals via thei...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx5002089
更新日期:2014-08-18 00:00:00
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
abstract::The DNA alkylating mechanism of PNU-159682 (PNU), a highly potent metabolite of the anthracycline nemorubicin, was investigated by gel-electrophoretic, HPLC-UV, and micro-HPLC/mass spectrometry (MS) measurements. PNU quickly reacted with double-stranded oligonucleotides, but not with single-stranded sequences, to form...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2017-02-20 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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journal_title:Chemical research in toxicology
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更新日期:2012-04-16 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2011-06-20 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00042a008
更新日期:1994-11-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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