Abstract:
OBJECTIVE:To evaluate the efficacy of detergent and friction on removal of traditional biofilm and cyclic-buildup biofilm (CBB) from polytetrafluoroethylene (PTFE) channels and to evaluate the efficacy of glutaraldehyde to kill residual bacteria after cleaning. METHODS:PTFE channels were exposed to artificial test soil containing 108 CFU/mL of Pseudomonas aeruginosa and Enterococcus faecalis, followed by full cleaning and high-level disinfection (HLD) for five repeated rounds to establish CBB. For traditional biofilm, the HLD step was omitted. Cleaning with enzymatic and alkaline detergents, bristle brush, and Pull Thru channel cleaner were compared to a water flush only. Carbohydrate, protein, viable count, adenosine triphosphate (ATP) levels were analyzed and atomic force microscopy (AFM) was performed. RESULTS:In the absence of friction, cleaning of traditional biofilm and CBB was not effective compared to the positive control (Dunn-Bonferroni tests; P > .05) regardless of the detergent used. ATP, protein, and carbohydrate analyses were unable to detect traditional biofilm or CBB. The AFM analysis showed that fixation resulted in CBB being smoother and more compact than traditional biofilm. CONCLUSION:Friction during the cleaning process was a critical parameter regardless of the detergent used for removal of either traditional biofilm or CBB. Glutaraldehyde effectively killed the remaining microorganisms regardless of the cleaning method used.
journal_name
Infect Control Hosp Epidemioljournal_title
Infection control and hospital epidemiologyauthors
Ribeiro MM,Graziano KU,Olson N,França R,Alfa MJdoi
10.1017/ice.2019.306subject
Has Abstractpub_date
2020-02-01 00:00:00pages
172-180issue
2eissn
0899-823Xissn
1559-6834pii
S0899823X19003064journal_volume
41pub_type
杂志文章abstract:OBJECTIVE:Surgical site infections (SSIs) are responsible for significant morbidity and mortality. Preadmission skin antisepsis, while controversial, has gained acceptance as a strategy for reducing the risk of SSI. In this study, we analyze the benefit of an electronic alert system for enhancing compliance to preadmis...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章,随机对照试验
doi:10.1017/ice.2015.303
更新日期:2016-03-01 00:00:00
abstract::We evaluated the impact of reflex urine culture screen results on antibiotic initiation. More patients with positive urine screen but negative culture received antibiotics than those with a negative screen (30.5 vs 7.1%). Urine screen results may inappropriately influence antibiotic initiation in patients with a low l...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2020.230
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:Evaluate the clinical impact of the implementation of VERIGENE gram-positive blood culture testing (BC-GP) coupled with antimicrobial stewardship result notification for children with positive blood cultures. DESIGN:Quasi-experimental study. SETTING:Quaternary children's hospital. PATIENTS:Hospitalized chi...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2020.191
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE:The effectiveness of hand-cleansing agents in removing a hospital strain of methicillin-resistant Staphylococcus aureus from artificially contaminated hands of five volunteers was studied. DESIGN:The products used were plain liquid soap, ethyl alcohol 70% (by weight), 10% povidone-iodine liquid soap (PVP-I),...
journal_title:Infection control and hospital epidemiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1086/501872
更新日期:2001-02-01 00:00:00
abstract::To ascertain the microbiology of skin abscesses, emergency department records were reviewed to identify patients with debrided skin abscesses. Methicillin-resistant Staphylococcus aureus was isolated from 255 (67.6%) of 377 culture samples from episodes in the adult cohort and from 145 (79.7%) of 182 culture samples f...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/516799
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVES:To determine the rates of surgical-site infections (SSIs) after spinal surgery and to identify the risk factors associated with infection. DESIGN:SSIs had been identified by active prospective surveillance. A case-control study to identify risk factors was performed retrospectively. SETTING:University-asso...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/502259
更新日期:2003-08-01 00:00:00
abstract::Results from prospective surveillance of nosocomial infections (NIs) in a small community hospital were evaluated, and a case-control study was conducted. The rate of 1.4 NIs per 100 discharges determined by prospective surveillance was found to be underestimated. Prematurity, pediatric service, surgery, length of sta...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/501920
更新日期:2001-06-01 00:00:00
abstract::An outbreak of metallo-β-lactamase (MBL) producing Klebsiella pneumoniae occurred at a children's hospital in Japan. MBL-producing K. pneumoniae was detected in tea dispenser in the hospital, the use of which was associated with the acquisition of the MBL-producing Enterobacteriaceae. The outbreak ceased after use of ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2018.331
更新日期:2018-12-27 00:00:00
abstract:BACKGROUND:States have established public reporting of hospital-associated (HA) infections-including those of methicillin-resistant Staphylococcus aureus (MRSA)-but do not account for hospital case mix or postdischarge events. OBJECTIVE:Identify facility-level characteristics associated with HA-MRSA infection admissio...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/678069
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To determine whether hydrogen peroxide vapor (HPV) decontamination can reduce environmental contamination with and nosocomial transmission of Clostridium difficile. DESIGN:A prospective before-after intervention study. SETTING:A hospital affected by an epidemic strain of C. difficile. INTERVENTION:Intensiv...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/589906
更新日期:2008-08-01 00:00:00
abstract::We investigated an outbreak of postoperative shoulder arthritis due to Propionibacterium acnes infection in nondebilitated patients. Risk factors were male sex, the order in which surgery was performed during the daily operating schedule, and increased duration of the surgical procedure. After modification of the vent...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/507287
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:Better understand the incidence, risk factors, and outcomes of peripheral venous catheter (PVC)-related Staphylococcus aureus bacteremia. DESIGN:Retrospective study of PVC-related S. aureus bacteremias in adult patients from July 2005 through March 2008. A point-prevalence survey was performed January 9, 200...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/660099
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVE:To elucidate behavioral determinants of handwashing among nurses. DESIGN:Statistical modeling using the Theory of Planned Behavior and relevant components to handwashing behavior by nurses that were derived from focus-group discussions and literature review. SETTING:The community and 3 tertiary care hospita...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/503335
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVE:To study colonization with methicillin-resistant Staphylococcus aureus in a home care service during a 4-month period. DESIGN:Prospective study. SETTING:A home care service located in Rio de Janeiro, Brazil. PARTICIPANTS:Patients admitted to the home care service during this period, their household contact...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/507921
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE:To determine hospital-related risk factors for surgical-site infection (SSI) following hip arthroplasty. DESIGN:Prospective, multicenter cohort study based on surveillance data and data collected through a structured telephone interview. With the use of multilevel logistic regression, the independent effect ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章,多中心研究
doi:10.1086/502564
更新日期:2005-05-01 00:00:00
abstract::The extent to which the use of alcohol-based handrub for hand hygiene has been adopted by US hospitals is unknown. A survey of infection control coordinators (response rate, 516 [72%] of 719) revealed that most hospitals (436 [84%] of 516) have adopted alcohol-based handrub. Leadership support and staff receptivity pl...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/592095
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:To investigate trends in plasmid types of methicillin-resistant Staphylococcus aureus over a 3-year period and determine the clinical impact of plasmid typing. DESIGN:Restriction endonuclease analysis of plasmid DNA (REAP) was used to type 270 clinical isolates. SETTING:A Veterans Affairs Medical Center. R...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/646627
更新日期:1993-01-01 00:00:00
abstract::We report the effect of prospective audit and feedback (PAF) on inpatient fluoroquinolone (FQN) prescriptions. During the PAF period, FQN use decreased from 39.19 to 29.58 days of therapy per 1,000 patient days (P < .001) and appropriateness improved from 68% to 88% (P < .001). High-yield indications to target include...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2020.339
更新日期:2020-12-01 00:00:00
abstract::OBJECTIVE Biofilm has been implicated in bacterial persistence and survival after endoscope reprocessing. In this study, we assessed the impact of different methods of reprocessing on organic residues and viable bacteria after repeated rounds of biofilm formation when each was followed by full reprocessing. METHODS AT...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2017.215
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:To describe the epidemiology and the interventions used to control two methicillin-resistant Staphylococcus aureus (MRSA) epidemics involving 46 infants with two fatalities in a neonatal intensive care unit (NICU). SETTING:A 50-bed, level III NICU in a university hospital. INTERVENTIONS:After traditional in...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/647285
更新日期:1996-04-01 00:00:00
abstract::The hospital-wide attack rate for Clostridium difficile-associated diarrhea at our tertiary-care university hospital was 0.02 per 100 patient discharges (0.02%) in 1982, but 0.41% and 1.47% in 1986 and 1987, respectively, with a peak incidence of 2.25% in the fourth quarter of 1987. Hospital antibiotic usage patterns ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/646173
更新日期:1990-06-01 00:00:00
abstract::OBJECTIVE National hospital performance measures for C. difficile infection (CD) are available; comparing antibacterial use among performance levels can aid in identifying effective antimicrobial stewardship strategies to reduce CDI rates. DESIGN Hospital-level, cross-sectional analysis. METHODS Hospital characteristi...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2016.87
更新日期:2016-08-01 00:00:00
abstract::OBJECTIVE To define how often methicillin-resistant Staphylococcus aureus (MRSA) is spread from resident to resident in long-term care facilities using whole-genome sequencing DESIGN Prospective cohort study SETTING A long-term care facility PARTICIPANTS Elderly residents in a long-term care facility METHODS Cultures ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1017/ice.2016.41
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:To determine whether disinfection protocols currently used for gastroscopes are effective against cultures of Tropheryma whipplei. DESIGN:The bactericidal activity of 2% glutaraldehyde and two peracetic acids on the Twist-Marseille strain of T. whipplei grown in cell monolayers was determined. PATIENTS:Two ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/502188
更新日期:2003-03-01 00:00:00
abstract::We evaluated vancomycin use in a hospital with endemic vancomycin-resistant enterococci and a vancomycin restriction program. Only 68% of vancomycin was prescribed appropriately. Inappropriate use was due primarily to empirical therapy. In the patients with a microbiological diagnosis following empirical therapy, 83% ...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/501548
更新日期:1999-01-01 00:00:00
abstract::OBJECTIVE. To quantify the association between admission to an intensive care unit (ICU) room most recently occupied by a patient positive for extended-spectrum β-lactamase (EBSL)-producing gram-negative bacteria and acquisition of infection or colonization with that pathogen. DESIGN. Retrospective cohort study. SETTI...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/670216
更新日期:2013-05-01 00:00:00
abstract::We used a survey to characterize contemporary infection prevention and antibiotic stewardship program practices across 64 healthcare facilities, and we compared these findings to those of a similar 2013 survey. Notable findings include decreased frequency of active surveillance for methicillin-resistant Staphylococcus...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章,多中心研究
doi:10.1017/ice.2019.172
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To screen copper and noncopper paints for their bactericidal effectiveness in rendering surfaces self-disinfecting. DESIGN:Tested paints were applied to glass coverslips, cured, inoculated with test organisms (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis), and dr...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/648361
更新日期:1995-08-01 00:00:00
abstract:OBJECTIVE:To determine trends in compliance with the guidelines for preventing the transmission of Mycobacterium tuberculosis in healthcare facilities among New Jersey hospitals from 1989 through 1996. DESIGN:A voluntary questionnaire was sent to all 96 New Jersey hospitals in 1992. The 53 that responded were resurvey...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/501627
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:To characterize the probability and duration of viral shedding among adults given trivalent live attenuated influenza vaccine (LAIV). DESIGN:Prospective surveillance study. METHODS:Nasal wash samples were collected from adult volunteers at baseline and on days 3, 7, and 10 and between days 17 and 21 followi...
journal_title:Infection control and hospital epidemiology
pub_type: 杂志文章
doi:10.1086/502574
更新日期:2005-05-01 00:00:00