Immunodetection in vivo of beet necrotic yellow vein virus-encoded proteins.

Abstract:

:Open reading frames identified on the four genomic RNAs of beet necrotic yellow vein virus were cloned into bacterial expression vectors and resulting cl-fusion proteins expressed in Escherichia coli were used to raise polyclonal antibodies. This set of antisera was used to show the presence of 7 of 9 predicted viral proteins in mechanically inoculated Chenopodium quinoa leaves by the Western blot technique. Viral coat protein (p22) and its readthrough protein p85 encoded by RNA-2 could be detected in all subcellular fractions. Two other RNA-2-encoded proteins, p42 and p13, are predominantly associated with membranous structures. Another RNA-2-encoded protein, p14, as well as the two polypeptides p25 and p31, encoded by RNA-3 and -4, respectively, are soluble proteins. The viral proteins could first be detected about the time lesions became visible and increased thereafter except for p85, in which case the amount of the soluble form decreased with time. No protein could be detected corresponding to the RNA-1-encoded p237 protein or to the p15 species encoded by open reading frame V of RNA-2.

journal_name

Virology

journal_title

Virology

authors

Niesbach-Klösgen U,Guilley H,Jonard G,Richards K

doi

10.1016/0042-6822(90)90378-5

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

52-61

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

178

pub_type

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