Advancing nanostructured porous si-based optical transducers for label free bacteria detection.

Abstract:

:Optical label-free porous Si-based biosensors for rapid bacteria detection are introduced. The biosensors are designed to directly capture the target bacteria cells onto their surface with no prior sample processing (such as cell lysis). Two types of nanostructured optical transducers based on oxidized porous Si (PSiO(2)) Fabry-Pérot thin films are synthesized and used to construct the biosensors. In the first system, we graft specific monoclonal antibodies (immunoglobulin G's) onto a neat electrochemically-machined PSiO(2) surface, based on well-established silanization chemistry. The second biosensor class consists of a PSiO(2)/hydrogel hybrid. The hydrogel, polyacrylamide, is synthesized in situ within the nanostructured PSiO(2) host and conjugated with specific monoclonal antibodies to provide the active component of the biosensor. Exposure of these modified-surfaces to the target bacteria results in "direct-cell-capture" onto the biosensor surface. These specific binding events induce predictable changes in the thin-film optical interference spectrum of the biosensor. Our studies demonstrate the applicability of these biosensors for the detection of low bacterial concentrations, in the range of 10(3)-10(5) cell/ml, within minutes. The sensing performance of the two different platforms, in terms of their stability in aqueous media and sensitivity, are compared and discussed. This preliminary study suggests that biosensors based on PSiO(2)/hydrogel hybrid outperform the neat PSiO(2) system.

journal_name

Adv Exp Med Biol

authors

Massad-Ivanir N,Shtenberg G,Segal E

doi

10.1007/978-94-007-2555-3_4

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

37-45

eissn

0065-2598

issn

2214-8019

journal_volume

733

pub_type

杂志文章
  • Role of complement in motor neuron disease: animal models and therapeutic potential of complement inhibitors.

    abstract::Amyotrophic lateral sclerosis (ALS) is one of the major forms of motor neuron disease (MND), a group of degenerative disorders causing progressive motor neuron death leading to eventual paralysis and death. The pathogenesis of MND is poorly understood and may include genetic and/or environmental factors, with a common...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:

    authors: Woodruff TM,Costantini KJ,Taylor SM,Noakes PG

    更新日期:2008-01-01 00:00:00

  • Hypoxic Adaptation in the Nervous System: Promise for Novel Therapeutics for Acute and Chronic Neurodegeneration.

    abstract::Homeostasis is the process by which cells adapt to stress and prevent or repair injury. Unique programs have evolved to sense and activate these homeostatic mechanisms and as such, homeostatic sensors may be potent therapeutic targets. The hypoxic response mediated by hypoxia inducible factor (HIF) downstream of oxyge...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4899-7678-9_16

    authors: Speer R,Ratan RR

    更新日期:2016-01-01 00:00:00

  • Intramyocardial mechanical states: vessel-interstitium-muscle interface.

    abstract::The intramural blood vessels and fluid-filled interstitial space form a hydraulic continuum enmeshed by myocardial muscle layers and collagen matrix. Coronary circulation was found to be strongly influenced by passive and active properties of the muscle surrounding the coronary microvasculature. Selective remodeling o...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4615-2946-0_11

    authors: Kresh JY

    更新日期:1993-01-01 00:00:00

  • Intramyocardial fluid transport effects on coronary flow and left ventricular mechanics.

    abstract::An integrated left ventricular (LV) model is used to solve, simultaneously and interactively, the LV mechanics, the coronary blood flow and capillary and interstitial fluid and mass transport, and to analyze the LV behavior under normal as well as pathological conditions. Accounting for the interstitial fluid mass bal...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4615-2946-0_21

    authors: Zinemanas D,Beyar R,Sideman S

    更新日期:1993-01-01 00:00:00

  • Understanding Peroxisome Proliferator-Activated Receptors: From the Structure to the Regulatory Actions on Metabolism.

    abstract::Peroxisome proliferator-activated receptors (PPARs) are multi-domains proteins, belonging to the superfamily of nuclear receptors, which mainly act as ligand-activated transcription factors. A variety of lipophilic molecules, including long-chain polyunsaturated fatty acids and eicosanoids, are capable of binding to P...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-3-030-11488-6_3

    authors: Lamas Bervejillo M,Ferreira AM

    更新日期:2019-01-01 00:00:00

  • Genetic variation in thymidylate synthase confers resistance to 5-fluorodeoxyuridine.

    abstract::The human colorectal tumor cell line HCT 116 was resident to the cytotoxic effects of 5-fluorodeoxyuridine (FdUrd). The response to FdUrd was increased only slightly by the presence of 10 microM folinic acid (CF). HCT 116 formed FdUMP and CH2H4PteGlu polyglutamates after exposure to FdUrd and CF. The sensitivity to Fd...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4615-2488-5_8

    authors: Hughey CT,Barbour KW,Berger FG,Berger SH

    更新日期:1993-01-01 00:00:00

  • The Production of a Rat Model That Inhibits Phosphoenolpyruvate Carboxykinase (PEPCK), a Rate-Limiting Enzyme of Hepatic Gluconeogenesis.

    abstract::We previously showed that taurine administration contributed to the extension of time to exhaustion through exercise-induced hypoglycemia restraint, and we suggested that the activation of hepatic gluconeogenesis was initiated before the exercise with the taurine administration. We hypothesize that the extension effec...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-981-13-8023-5_10

    authors: Ohmori H,Matsumura M,Komine S,Kobayashi H,Kobayashi Y,Shiromoto J,Miyakawa S

    更新日期:2019-01-01 00:00:00

  • Antibodies and Selection of Monoclonal Antibodies.

    abstract::Monoclonal antibodies are universal binding molecules with a high specificity for their target and are indispensable tools in research, diagnostics and therapy. The biotechnological generation of monoclonal antibodies was enabled by the hybridoma technology published in 1975 by Köhler and Milstein. Today monoclonal an...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-3-319-32805-8_2

    authors: Hanack K,Messerschmidt K,Listek M

    更新日期:2016-01-01 00:00:00

  • The action of benzbromarone in relation to age, sex and accompanying diseases.

    abstract::The uric-acid lowering effect was investigated in a group of 2220 patients. 1984 of these were employed for statistical evaluation purposes. On average, the uric acid level was reduced from 8,24 +/- 1,16 mg/100 ml to 5,32 +/- 1,265 mg/100 ml. In 82 % of all the cases, the uric acid level at the end of the treatment pe...

    journal_title:Advances in experimental medicine and biology

    pub_type: 临床试验,杂志文章

    doi:10.1007/978-1-4615-9140-5_47

    authors: Ferber H,Bader U,Matzkies F

    更新日期:1980-01-01 00:00:00

  • Emerging issues for tropomyosin structure, regulation, function and pathology.

    abstract::There is a growing awareness of the role of tropomyosin in the regulation of the actin filament. Work in the field is increasingly directed at understanding the mechanisms of function at both a molecular and atomic level and developing therapeutic strategies to treat tropomyosin-based pathology. This chapter highlight...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-0-387-85766-4_22

    authors: Gunning P

    更新日期:2008-01-01 00:00:00

  • Investigation of frontal cortex, motor cortex and systemic haemodynamic changes during anagram solving.

    abstract::We have previously reported changes in the concentrations of oxy-(delta[HbO2]) deoxy- (delta[HHb]) and total haemoglobin (delta[HbT] = delta[HbO2] + delta[HHb]) measured using near infrared spectroscopy (NIRS) over the frontal cortex (FC) during an anagram solving task. These changes were associated with a significant...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-0-387-74911-2_3

    authors: Tachtsidis I,Leung TS,Tisdall MM,Devendra P,Smith M,Delpy DT,Elwell CE

    更新日期:2008-01-01 00:00:00

  • Mitochondrial diseases: a cross-talk between mitochondrial and nuclear genomes.

    abstract::More than one billion years ago, mitochondria were free-living prokaryotic organisms with their own DNA. However, during the evolution, ancestral genes have been transferred from the mitochondrial to the nuclear genome so that mtDNA became dependent on numerous nucleus-encoded factors for its integrity, replication an...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-90-481-2813-6_6

    authors: Spinazzola A,Zeviani M

    更新日期:2009-01-01 00:00:00

  • A reappraisal of the mechanisms of hypocholesterolemic action of therapeutic agents.

    abstract::The most commonly used methods to study the mechanisms of hypocholesterolemic action of therapeutic agents generally determine the turnover of total (exchangeable) cholesterol pools in the body. This approach is based on the view that whatever increases the total load of cholesterol in the body will increase the level...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4684-0967-3_19

    authors: Sodhi HS,Kudchodkar BJ,Clifford C,Borhani N,Mason DT

    更新日期:1978-01-01 00:00:00

  • Modification of carcinogen metabolism by indolylic autolysis products of Brassica oleraceae.

    abstract::Cruciferous plant foods contain large quantities of secondary plant metabolites that have been shown to inhibit chemically induced carcinogenesis in animals. One mechanism by which these chemicals may inhibit carcinogenesis is through the induction of enzymes, such as cytochrome P-450-dependent monooxygenases, glutath...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4899-2626-5_13

    authors: Bradfield CA,Bjeldanes LF

    更新日期:1991-01-01 00:00:00

  • Dose-dependent inhibition of aspartate carbamoyltransferase in peripheral blood mononuclear cells in patients receiving N-(phosphonacetyl)-L-aspartate.

    abstract::Forty-eight patients with adenocarcinoma of the gastrointestinal tract were treated on this trial. The MTD of 5-FU given as a 72 hour infusion with high-dose leucovorin was initially determined to be 2000 mg/m2/d. Patients were treated at PALA dose levels ranging from 250 to 2848 mg/m2. Biochemical assessment of targe...

    journal_title:Advances in experimental medicine and biology

    pub_type: 临床试验,杂志文章

    doi:10.1007/978-1-4615-2488-5_13

    authors: Grem JL,McAtee N,Drake JC,Steinberg S,Allegra CJ

    更新日期:1993-01-01 00:00:00

  • Serum tryptophan and 5-hydroxytryptophan at birth and during post-partum days.

    abstract::Serum levels of tryptophan (Trp) and 5-hydroxytryptophan (5-HTP) were measured in the mother at birth, by normal delivery or cesarean section, on days 1 and 3 post-partum, and in the cord blood of the fetus-newborns. Decreases in Trp and 5-HTP levels during delivery were noted. Conversely, Trp levels in the cord blood...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4615-0135-0_90

    authors: Carretti N,Bertazzo A,Comai S,Costa CV,Allegri G,Petraglia F

    更新日期:2003-01-01 00:00:00

  • Regulation of Hsf1 and the Heat Shock Response.

    abstract::The heat shock response (HSR) is characterized by the induction of molecular chaperones following a sudden increase in temperature. In eukaryotes, the HSR comprises the set of genes controlled by the transcription factor Hsf1. The HSR is induced by defects in co-translational protein folding, ribosome biogenesis, orga...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-3-030-40204-4_3

    authors: Pincus D

    更新日期:2020-01-01 00:00:00

  • How do bone cells secrete proteins?

    abstract::The ruffled border is the most specific marker of the active osteoclast (OC) as it forms only when the cell is resorbing bone. We provide evidence that this complex cytoskeletal structure reflects insertion of the lysosomal vesicles into the bone-apposed plasma membrane under the aegis of the Ca-sensing, exocytic prot...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4419-1050-9_11

    authors: Zhao H,Ito Y,Chappel J,Andrews N,Ross FP,Teitelbaum SL

    更新日期:2010-01-01 00:00:00

  • Opposite Roles of MerTK Ligands Gas6 and Protein S During Retinal Phagocytosis.

    abstract::MerTK is required for photoreceptor outer segment (POS) phagocytosis by retinal pigment epithelial (RPE) cells, a diurnal function essential for vision maintenance. In vivo, MerTK is stimulated at the time of the phagocytic peak through an intracellular signaling pathway. However, MerTK ligands Gas6 and Protein S are ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-3-319-75402-4_70

    authors: Nandrot EF

    更新日期:2018-01-01 00:00:00

  • Genetic Evolution of Hepatitis E Virus.

    abstract::Comparative analysis of the genomic sequences of multiple hepatitis E virus (HEV) isolates has revealed extensive genomic diversity among them. Recently, a variety of genetically distinct HEV variants have also been isolated and identified from large numbers of animal species, including birds, rabbits, rats, ferrets, ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-94-024-0942-0_5

    authors: Zhang Y,Gong W,Zeng H,Wang L

    更新日期:2016-01-01 00:00:00

  • Canonical correlation analysis in the study of cerebral and peripheral haemodynamics interrelations with systemic variables in neonates supported on ECMO.

    abstract::Neonates supported on extracorporeal membrane oxygenation (ECMO) are at high risk of brain injury due to haemodynamic instability. In order to monitor cerebral and peripheral (muscle) haemodynamic and oxygenation changes in this population we used a dual-channel near-infrared spectroscopy (NIRS) system. In addition, t...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4614-4989-8_4

    authors: Caicedo A,Papademetriou MD,Elwell CE,Hoskote A,Elliott MJ,Van Huffel S,Tachtsidis I

    更新日期:2013-01-01 00:00:00

  • Fibrin and the vessel wall.

    abstract::Fibrin is a major component of atherosclerotic plaques, and there may also be situations in which intravascular fibrin is formed in contact with the endothelium. The studies to be presented describe the distribution of fibrinogen/fibrin I, fibrin II, and fragments D and D-dimer in normal vessels and atherosclerotic pl...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4615-3806-6_33

    authors: Kaplan KL,Bini A,Fenoglio J Jr,Kudryk B

    更新日期:1990-01-01 00:00:00

  • Lipid A receptor TLR4-mediated signaling pathways.

    abstract::Lipid A is a strong activator of monocytes to release immune stimulators such as proinflammatory cytokines. Overproduction of inflammatory cytokines such as TNF and IL-6 is known to cause septic shock that frequently leads to multiple organ failure and finally to death. In recent years, Lipid A has also been recognize...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-1-4419-1603-7_6

    authors: Yamamoto M,Akira S

    更新日期:2010-01-01 00:00:00

  • A Mini Review: Moving iPSC-Derived Retinal Subtypes Forward for Clinical Applications for Retinal Degenerative Diseases.

    abstract::Patient-derived human-induced pluripotent stem cells (iPSCs) have been critical in advancing our understanding of the underlying mechanisms of numerous retinal disorders. Many of these retinal disorders have no effective treatment and result in severe visual impairment and even blindness. Among the retinal degenerativ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-3-030-27378-1_91

    authors: Cho C,Duong TT,Mills JA

    更新日期:2019-01-01 00:00:00

  • Astroglia in Leukodystrophies.

    abstract::Leukodystrophies are genetically determined disorders affecting the white matter of the central nervous system. The combination of MRI pattern recognition and next-generation sequencing for the definition of novel disease entities has recently demonstrated that many leukodystrophies are due to the primary involvement ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-981-13-9913-8_9

    authors: Jorge MS,Bugiani M

    更新日期:2019-01-01 00:00:00

  • Sleep Disorders and Adherence to Inhalation Therapy in Patients with Chronic Obstructive Pulmonary Disease.

    abstract::Sleep disorders are common in patients with chronic obstructive pulmonary disease (COPD) and are associated with greater disease severity, more frequent exacerbations, greater use of emergency health care, and higher mortality. They may contribute to worsening of COPD course by reducing patients' adherence to treatmen...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/5584_2019_345

    authors: Chabowski M,Łuczak J,Dudek K,Jankowska-Polańska B

    更新日期:2019-01-01 00:00:00

  • Effects of the polyamine spermine on arterial chemoreception.

    abstract::Polyamines modulate many biological functions. Here we report a novel inhibitory modulation by spermine of catecholamine release by the rat carotid body and have identified the molecular mechanism underpinning it. We used molecular (RT-PCR and confocal microscopy) and functional (i.e., neurotransmitter release, patch ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-90-481-2259-2_11

    authors: Cayzac S,Rocher A,Obeso A,Gonzalez C,Kemp PJ,Riccardi D

    更新日期:2009-01-01 00:00:00

  • Control of muscle synergies by cortical ensembles.

    abstract::Since its introduction in the early 1980s, the concept of a "preferred direction" for neuronal discharge has proven to be a powerful means of studying diverse properties of individual neurons in the motor areas of the brain. More recently, the activity recorded from ensembles of neurons, each with an identified prefer...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章

    doi:10.1007/978-0-387-77064-2_9

    authors: Morrow MM,Pohlmeyer EA,Miller LE

    更新日期:2009-01-01 00:00:00

  • Actin-bound nucleotide/divalent cation interactions.

    abstract::At this point, it may be worthwhile to list, in summary form, the important aspects of divalent cation and nucleotide binding to actin that have been reviewed here: 1) High affinity divalent cation binding to actin is very tight, with equilibrium dissociation constant KCa approximately 1 nM and KMg approximately 5 nM ...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4615-2578-3_4

    authors: Gershman LC,Selden LA,Kinosian HJ,Estes JE

    更新日期:1994-01-01 00:00:00

  • In vitro transformation of monocytes and dendritic cells into endothelial like cells.

    abstract::Our in vitro data indicate that peripheral blood monocytes or monocyte-derived immature dendritic cells under appropriate culture conditions transdifferentiate into endothelial-like cells (ELC), which are characterized by the expression of endothelial markers and the formation of tube-like structures. Dependent on the...

    journal_title:Advances in experimental medicine and biology

    pub_type: 杂志文章,评审

    doi:10.1007/978-1-4615-0169-5_6

    authors: Havemann K,Pujol BF,Adamkiewicz J

    更新日期:2003-01-01 00:00:00