Abstract:
AIM:To study levels of hepsidine (Hp), hypoxia-inducible factor-1alpha (HIF-1alpha), erythropoietin (EP) and ferritin in patients with acute leukemia (AL), effects of protein fractions of homogenate of blastic cells (BC) on regulatory proteins studied. MATERIAL AND METHODS:Depending on leukocyte count in the onset of AL, 70 patients with first ever diagnosed AL were divided into two groups: group 1 - 17 patients with leukocyte count > 30 x 10(9)/l, group 2 - 53 patients with leukocyte count < 30 x 10(9)/l. Serum and leukocyte parameters were studied before treatment, during the treatment with cytostatic drugs, in the course of myelotoxic agranulocytosis (MTA), after normalization of hemograms. EP was detected with enzyme immunoassay, serum and leukocyte ferritin - with radioimmunoassay; HIF-1alpha, Hp - sandwich enzyme immunoassay using monospecific antisera and monoclonal antibodies against relevant antigens. Leukocytes were isolated in ficol and verografin solutions density gradient. Chromatographic division of the protein fractions in 3 patients with leukocytosis in AL onset and leukocytes of 3 donors was made by the method of preparative isoelectrofocusing of BC and leukocytes on LKB colon (Sweden). Effects of these fractions were studied on 11 plasma samples from hematological patients and 4 plasma samples from patients with normal hemopoiesis. RESULTS:Leukocytosis patients with initial AL have serum ferritin, Hp and HIF-1alpha levels about 2-5 times lower than patients without leukocytosis. Cytostatic treatment raises an HIF-1alpha level in BC about 15-fold (85.8 +/- 24.5 pg/ml), in the study group - 3-fold (15.2 +/- 3.3 pg/ml). The highest EP levels were seen in MTA. It is detected that protein fractions isolated from leukocytes of patients with leukocytosis in the disease onset raise HIF-1alpha content irrespective of HIF-1alpha presence in the fraction. Patients free of hematological diseases have no changes of the above proteins. CONCLUSION:Great difficulties exist in ferritin and Hp levels between AL patients with leukocytosis in the onset of the disease and without of leukocytosis. The study of leukocytes suggests that tumor cells of such patients contain compounds which can regulate production of HIF-1alpha, Hp and ferritin.
journal_name
Ter Arkhjournal_title
Terapevticheskii arkhivauthors
subject
Has Abstractpub_date
2011-01-01 00:00:00pages
22-7issue
10eissn
0040-3660issn
2309-5342journal_volume
83pub_type
杂志文章abstract::Sarcoidosis is a multisystem granulomatous disease of unknown etiology. Liver is frequently involved in the pathological process. Wide range of clinical manifestations can be seen: from asymptomatic lesion with normal liver function tests to cirrhosis with portal hypertension. Biopsy plays the key role in diagnosis of...
journal_title:Terapevticheskii arkhiv
pub_type: 社论
doi:10.26442/00403660.2019.04.000179
更新日期:2019-05-15 00:00:00
abstract::The paper summarizes the data of international and Russian studies concerning current approaches to the pharmacotherapy of rheumatoid arthritis. Particular emphasis is placed on the substantiation of the treat-to-target concept, on the efficacy and safety of genetically engineered biological agents, including the inhi...
journal_title:Terapevticheskii arkhiv
pub_type: 历史文章,杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract::Forty-one patients suffering from chronic systemic lupus erythematosus with Raynaud and Sjögren's syndromes and 18 patients with an acute and subacute disease course were examined. It was established that chronic SLE was marked by a high frequency of antibodies to ribonucleoprotein, an increase in the concentration of...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:AIM:To analyze the influence of clinical and pharmacogenetic factors on the risk of resistance to original or generic clopidogrel and that of cardiovascular events (CVE) during 12 months of follow-up. SUBJECTS AND METHODS:Two hundred and fifty patients admitted to Moscow hospitals in October 2011 to September 2012 wer...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::The efficacy of allapinin, ethacizine, ritmilen, kinilentin, obsidan and finoptin given alone and in different combinations was studied in 44 patients with paroxysmal atrial fibrillation provoked by transesophageal pacing. The use of the combinations of the drugs belonging to the first group as well of those of the fi...
journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1992-01-01 00:00:00
abstract::The authors present literature data and original findings on characteristics and frequency of hematological disorders in SLE. Marked hemopathies are called SLE masks as in primary examination of the patient systemic blood diseases are not rejected this necessitating special investigations to exclude them. A rare case ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract:AIM:To analyse clinical implications of chromosome 8 trisomy in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia (CML) treated with inhibitors of tyrosinkinases (ITK). MATERIAL AND METHODS:A total of 386 patients with CML (chronic phase--288, acceleration phase--77) received imatinib (400...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract:AIM:To investigate the AGTR1 A/C polymorphism associated with atrial fibrillation (AF) to form risk groups among patients who are prone to this disease. SUBJECTS AND METHODS:90 probands with a confirmed diagnosis of AF and their 144 first-, second-, and third-degree relatives were examined. These families made up a st...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.17116/terarkh201789948-52
更新日期:2017-01-01 00:00:00
abstract::A study was made of the periodical motor activity (PMA) of the antral part of the stomach (APS) and duodenum (D) using a manometric system of 4 continuously perfused catheters in 96 patients with peptic ulcer, 20 patients with ulcer of the body of the stomach, 26--with chronic pancreatitis, and in 16 healthy controls....
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The authors analyze the results of the surgical treatment of patients with progressive essential hypertension (PEN). The treatment involved cryodestruction (adrenalectomy) of the right adrenal and autotransplantation of the left adrenal on the vascular peduncle into the transverse mesentery. Before the operation the d...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:AIM:to study the time course of changes in the gastric juice levels of nitric oxide (NO) in patients with esophagogastroduonenal diseases (EGDD). SUBJECTS AND METHODS:One hundred and seventy-four patients with EGDD were examined. RESULTS:In patients with chronic atrophic gastritis, the level of NO was significantly l...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::A total of 48 patients with calculous pyelonephritis were examined before and after surgery. A study was made of the fibrinolytic system (according to the status of XIIa kallikrein-dependent fibrinolysis, lysis of the plasma clots and euglobulin lysis) and of the kallikrein-kinin system of the plasma (according to kal...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::The paper is concerned with an organizational, structural and functional model of the diagnostic center (DC) in the train, developed by the authors. The DC in the train is a new form of mobile specialized polyclinical aid to the population living in the area of railway stations. The purpose of the model is to draw nea...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Toxic patterns of diphtheria of the fauces in adults are characterized by laryngeal lesions which considerably deteriorate the disease prognosis. The clinical manifestations of diphtheric croup may be unpronounced in such cases and be masked by other severe disease symptoms. If patients with toxic diphtheria develop c...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:AIM:To study efficacy of ANCA-RPGN treatment with corticosteroids and cyclophosphamide or mycophenolic acid drugs. MATERIAL AND METHODS:We treated 28 patients (17 males and 11 females aged 19-71 years) with morphologically verified ANCA-associated crescentic RPGN (crescentic median 79 (63:88)%. The patients received c...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract:AIM:To study morphofunctional peculiarities of gastric mucosa in native and migrant rural population of North Siberia. MATERIAL AND METHODS:A comprehensive clinicomorphological examination including physical examination, ultrasonic scanning, cholecystography, duodenal intubation, intragastric pH-metry, esophagogastrod...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Levels of R proteins (RP) and activity of lipid peroxidation (LPO) were measured in 209 patients with RA. It was noted that RP levels and LPO activity correlated with the activity of inflammation. A rapid RA progression was characterized by higher RP levels and LPO activity, while polyarthritis by more intensive RP me...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Deciphering immunopathogenesis, expanding the scope of diagnostics and developing new methods for treating human autoimmune diseases are among the priority areas of XXI century medicine. Particularly widely autoimmune pathology is presented in immunoinflammatory rheumatic diseases (IIRD), such as rheumatoid arthritis,...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/00403660.2019.08.000406
更新日期:2019-08-15 00:00:00
abstract::The paper is concerned with the role played by interferon (IF) in rheumatoid arthritis (RA). The content of IF was measured in the patients' blood serum and the possibility of the use of the preparations of exogenous interferon for the treatment of RA patients was demonstrated. Assay of serum IF in RA patients showed ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:AIM:To evaluate telmisartan effects on 24-h blood pressure profile, sensitivity to insulin, carbohydrate and lipid metabolism in patients with metabolic syndrome (MS) and arterial hypertension (AH). MATERIAL AND METHODS:Twenty patients with MS and AH of the degree I-II received telmisartan in a dose 80 mg/day. 24-h BP...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:AIM:To devise the technique and evaluate the efficacy of videothoracoscopic collagen pleurodesis (VCP) in malignant pleural exudates (MPE). MATERIAL AND METHODS:VCP was performed in 24 patients with recurrent MPE. 20 patients were diagnosed to have non-small-cell cancer of the lung, 4 patients--breast cancer. The oper...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:AIM:To assess changes in quality of life (QL) and cognitive functions in patients with end-stage renal failure (ESRF) treated with hemodialysis using a succinate-containing dialyzing solution (SCDS). SUBJECTS AND METHODS:Sixty-seven patients with ESRF on hemodialysis using SCDS were examined. The investigators determi...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:AIM:To compare efficacy and tolerability of combined therapy with methotrexate (MTX), sulfasalazine (SSZ) and hydroxychloroquine (HCQ) with MTX-monotherapy in patients with rheumatoid arthritis (RA). MATERIAL AND METHODS:RA patients (n = 60) who had not been treated with the above drugs were randomized (1:1) to receiv...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2008-01-01 00:00:00
abstract:AIM:A comparative study of efficiency and safety of low-dose erythropoietin (EP) in two groups of patients with chronic renal failure (CRF): patients on chronic hemodialysis (CHD) and patients on continuous ambulatory hemodialysis (CAHD). MATERIALS AND METHODS:51 CRF adult patients with renal anemia on hemodialysis en...
journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:AIM:To characterize a superficial phenotype and to make a cytogenetic analysis of bone marrow (BM) mesenchymal stromal cells (MSC) from donors. MATERIALS AND METHODS:The study analyzed BM samples from 11 healthy donors. The phenotype of obtained MSC was analyzed using cytofluorometry. Chromosomal analysis was carried ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::A study was made of the capacity of peritoneal macrophages to demonstrate the atherogenic potential of blood serum. Murine and human macrophages were found to be capable of intracellular accumulation of cholesterol during cultivation with blood sera from patients with coronary atherosclerosis. The data on the capacity...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:AIM:To investigate a potential of a new marker of activity of interstitial pulmonary diseases (IPD)--mucin 3EG5--in monitoring of effectiveness of the antiinflammatory therapy. MATERIALS AND METHODS:Serum mucin antigen 3EG5 (glycolyl-sialylated glycoprotein) was assessed with sandwich immunoassay constructed using a m...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The relevance of investigating carbohydrate metabolism (CM) in patients with acromegaly and Itsenko-Cushing disease is attributable to frequent glucose metabolic disturbances, on the one hand, and to difficulties in choosing sugar-lowering therapy in these categories of patients, on the other. The efficiency of hyperg...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.17116/terarkh2015871098-104
更新日期:2015-01-01 00:00:00
abstract::A significant progress in the field of molecular-biological investigations resulted in definition of a new group of systemic diseases referred to as autoinflammatory. This group comprises familial periodic fevers: periodic disease (mediterranean fever), Muckle-Wells syndrome, others cryopirinopathy, TRAPS-syndrome. As...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:AIM:To study clinical characteristics of non-ST-segment elevation acute coronary syndrome (NSTEACS) and to assess the quality of medical health in patients with this condition who were treated in the regional vascular centers and primary vascular units from 48 subjects of the Russian Federation in 2009-2012. MATERIALS...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00