Abstract:
:Recent advances in culturing of intestinal stem cells and pluripotent stem cells have led to the development of intestinal organoids. These are self-organizing 3D structures, which recapitulate the characteristics and physiological features of in vivo intestinal epithelium. Intestinal organoids have allowed the development of novel in vitro models to study various gastrointestinal diseases expanding our understanding of the pathophysiology of diseases and leading to the development of innovative therapies. This article aims to summarize the current usage of intestinal organoids as a model of gastrointestinal diseases and the potential applications of intestinal organoids in infants and children. Intestinal organoids allow the study of intestinal epithelium responses to stress factors. Mimicking intestinal injury such as necrotizing enterocolitis, intestinal organoids increases the expression of pro-inflammatory cytokine genes and shows disruption of tight junctions after they are injured by lipopolysaccharide and hypoxia. In cystic fibrosis, intestinal organoids derived from rectal biopsies have provided benefits in genetic studies and development of novel therapeutic gene modulation. Transplantation of intestinal organoids via enema has been shown to rescue damaged colonic epithelium in mice. In addition, tissue-engineered small intestine derived from intestinal organoids have been successfully established providing a potential novel treatment and a new hope for children with short bowel syndrome.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Chusilp S,Li B,Lee D,Lee C,Vejchapipat P,Pierro Adoi
10.1007/s00383-019-04581-3subject
Has Abstractpub_date
2020-01-01 00:00:00pages
1-10issue
1eissn
0179-0358issn
1437-9813pii
10.1007/s00383-019-04581-3journal_volume
36pub_type
杂志文章,评审abstract::The successful management of faecal incontinence can dramatically improve the quality of life of affected children. The introduction of the non-refluxing, catheterisable appendico-caecostomy provides the opportunity to treat previously resistant patients. Over a 6-year period, 29 children had a Malone antegrade contin...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050342
更新日期:1998-07-01 00:00:00
abstract:PURPOSE:To study the effects of frenulotomy on nipple pain, latch and the success in exclusive breastfeeding (EBF) at 3-month follow-up. METHODS:A prospective cross-sectional study of 328 mother-infant pairs with both tongue-tie and breastfeeding problems was performed. Nipple pain and latch were evaluated using numer...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3952-8
更新日期:2016-10-01 00:00:00
abstract::A previous study documented the effects of maternal corticosteroid treatment on structural growth and functional development in fetal lungs associated with pathogenic conditions such as oligohydramnios using immunohistochemical and morphometric analyses. The purpose of the present study was to examine the effect of ma...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1979-6
更新日期:2007-10-01 00:00:00
abstract::We review a single surgeon and surgical centre's experience with congenital cystic adenomatoid malformation of the lung (CCAML) in relation to clinical spectrum, operative experience, and postoperative course. A retrospective hospital record review was done on surgically treated cases of CCAML over a 10-year period, f...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1225-4
更新日期:2004-07-01 00:00:00
abstract::A case of isolated Caroli's disease in a 2-year-old female presented as an exophytic cystic mass of the liver. A palpable mass below the right costal margin and displacement of the right colon were the main points of clinical presentation. Preoperatively, sonographic signs of intraluminal vascular tracts were identifi...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050281
更新日期:1998-03-01 00:00:00
abstract::Nitrofen induces in rats diaphragmatic hernia (CDH) with heart and lung hypoplasia by a mechanism involving oxidation. The aim of this study was to examine if prenatal administration of the anti-oxidant agent vitamin E (VitE) prevents to some extent heart and lung hypoplasia. Pregnant rats received on E9.5 either 100 ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1005-6
更新日期:2003-07-01 00:00:00
abstract::Our presentation of four cases demonstrates the essential features of limb-body wall complex (LBWC), representing a compound anomaly pattern in body-wall defects. The diagnosis of this entity is based on two of the three following characteristics: (1) exencephaly/encephalocele and facial clefts; (2) thoraco- and/or ab...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000497
更新日期:2001-07-01 00:00:00
abstract::Children and adolescents affected by ulcerative colitis (UC) frequently require colectomy because of refractory or chronic symptoms. The aim of this paper is to present our experience and 10-year follow-up results of 28 patients who underwent endorectal pull-through (ERPT) as surgical treatment for UC, with special re...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1232-5
更新日期:2004-08-01 00:00:00
abstract:PURPOSE:The purpose of this study was to test the susceptibility of human hepatoblastoma and neuroblastoma cells to photodynamic diagnostics (PDD) and photodynamic therapy (PDT) using 5-aminolevulinic acid (5-ALA) as a photosensitizer. METHODS:Cell cultures of human hepatoblastoma (HuH6) and neuroblastoma (MHH-NB-11) ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2275-9
更新日期:2008-12-01 00:00:00
abstract::It is unknown whether noncomplicated acute appendicitis cause bacterial translocation. In this study, we aimed to test development of the bacterial translocation in the patients who were operated for acute appendicitis. In this prospective study, 10 control patients who underwent elective operations because of other r...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1899-5
更新日期:2007-06-01 00:00:00
abstract::Congenital lumbar hernia (CLH) is a rare anomaly with only 45 cases reported in the English-language literature. This paper describes nine patients with CLH treated in our unit. Unusual features included the relatively high incidence of inferior lumbar hernia, presentation at the age of 6 years in one case, and an ass...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050048
更新日期:2000-01-01 00:00:00
abstract::A reproducible technique was developed experimentally in pigs for construction of a 'composite bowel tube' (CBT) made up of ileal mucosa that was grafted to a mucosally denuded colonic muscle surface vascularised by the colonic mesentery. Macroscopic and microscopic studies at terminal laparotomy revealed a viable, mu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00178430
更新日期:1996-04-01 00:00:00
abstract:PURPOSE:The optimal management of simple hepatic cysts (SHCs) remains controversial. A retrospective review was carried out to evaluate the clinical presentation and surgical consideration of children with SHCs and outcome of management at our institution. METHODS:We performed a retrospective review of all patients di...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3509-7
更新日期:2014-06-01 00:00:00
abstract:AIMS AND OBJECTIVES:Vascularized bio-engineered human dermo-epidermal skin substitutes (vascDESS) hold promise for treating burn patients, including those with severe full-thickness wounds. We have previously shown that vascDESS promote wound healing by enhanced influx of macrophages and granulocytes. Immediately follo...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4179-z
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:We evaluated the effectiveness of prophylactic mesenteric lymphatic ligation in preventing iatrogenic chylous fistulae in abdominal neuroblastoma surgery. METHOD:All patients who underwent resection of abdominal neuroblastoma between years 2002 and 2013 were included. A change in surgical strategy commenced fr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3581-z
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:To review the effectiveness of the longitudinal intestinal lengthening and tailoring (LILT) and serial transverse enteroplasty (STEP) operations in a cohort of patients with short bowel syndrome (SBS). METHODS:We conducted a retrospective analysis of children with SBS treated at our institution from 2004 until...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04468-3
更新日期:2019-06-01 00:00:00
abstract::Adrenal cysts (AC) are rare, particularly in the newborn period. Almost all ACs in newborns were diagnosed on exploratory laparotomy or at autopsy. We report a newborn with an AC that was diagnosed after an exploratory laparotomy due to its rare presentation. This case shows that ACs should be considered in the differ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0759-6
更新日期:2003-06-01 00:00:00
abstract:PURPOSE:Chronological age has not always been an accurate predictor of gut motility in newborns. We hypothesized that the enteric plexus is immature at birth. We studied whole-mount preparations of the myenteric plexus in pigs from mid-point of gestation to adulthood. METHODS:Distal large bowel whole-mount preparation...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2265-y
更新日期:2008-12-01 00:00:00
abstract:PURPOSE:Pediatric genitalia injury represents 0.6% of all pediatric trauma. It is crucial for providers to understand whether pediatric patients are at risk for violent mechanisms, such as rape, assault, or other abuse. Therefore, we sought to perform a large database analysis of pediatric and adolescent female genital...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04736-7
更新日期:2020-10-01 00:00:00
abstract::Pulmonary sequestration is a rare congenital pulmonary malformation. We report a case of a 10-month-old infant with intralobar pulmonary sequestration diagnosed in utero. The lesion had an uncommon blood supply consisting of three large arteries deriving from the thoracic aorta and venous drainage into the inferior ve...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3088-4
更新日期:2012-07-01 00:00:00
abstract::True duplicate exstrophy is a rare and interesting congenital anomaly. We are adding a case to the previously reported seven cases in the world literature. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s003830000557
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:To compare open repair (OR) with thoracoscopic repair (TR) for congenital diaphragmatic hernia (CDH) in neonates. METHODS:Twenty-four neonatal CDH cases diagnosed prenatally or within 6 h of birth at our institute from 2002 to 2012 with mild pulmonary hypertension managed without inhaled nitric oxide, were stu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3382-9
更新日期:2013-11-01 00:00:00
abstract:AIMS:The aim of this study was to assess the usefulness of PAS, β-catenin and Ki-67 in categorising hepatoblastomas (HBs) and their significance in predicting prognosis. In addition, we have also documented the various chemotherapy induced histologic changes in HBs. METHOD:In this retrospective observational study of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4115-2
更新日期:2017-09-01 00:00:00
abstract::Parasitic infestations, mainly enterobiasis and amoebiasis, and poor toilet training practices are commonly associated with rectal prolapse in developing countries. Injection sclerotherapy is one of the commonly used modalities for treating partial rectal prolapse in children. Various materials are available for such ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1197-4
更新日期:2004-05-01 00:00:00
abstract::The majority of congenital diaphragmatic hernias (CDH) occur through the foramen of Bochdalek; herniation through the foramen of Morgagni (MH) is rare. Fifteen children (12 males and 3 females) with congenital MH (7 right, 3 left, 5 bilateral) were treated over a period of 15 years, comprising 11% of a total of 135 ch...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100653
更新日期:2002-03-01 00:00:00
abstract::Glicentin, the main component of enteroglucagon, has trophic effects on intestinal mucosa. It may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. We have established an in vitro bioassay system for determining the effects of recombinant human glicentin on bacterial internalization by co...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1895-9
更新日期:2007-06-01 00:00:00
abstract::Antral web is a rare cause of gastric-outlet obstruction. We report a case of gastric antral web with intermittent vomiting in a 10-year-old girl who received medical treatment for 6 months. The literature on this anomaly is reviewed with respect to differential diagnosis on the basis of upper gastrointestinal radiogr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003839900317
更新日期:2000-01-01 00:00:00
abstract::This report describes three children, age range 7 weeks to 5 months, who presented with obstructive jaundice secondary to gallstones. Previous Escherichia coli septicaemia and frusemide therapy were predisposing risk factors in two of the patients. All three were successfully treated with cholecystectomy and explorati...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Oesophageal atresia (OA) is often associated with anomalies of other systems. The genetic contribution to the formation of the VACTERL association is not clear. The objective of this study was to evaluate the incidence of associated anomalies in two different racial populations. The associated anomalies in neonates wi...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100692
更新日期:2002-05-01 00:00:00
abstract::Three cases of neonatal chylous ascites (CCA) were managed in the neonatal unit, University Hospital, Kuala Lumpur, over the past 9 years. Fetal ascites and polyhydramnios were the sole abnormalities detected in all three babies by antenatal ultrasonography. They were born at 36 weeks' gestation and their birth weight...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/BF01372154
更新日期:1997-04-01 00:00:00