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Bioguided Fractionation, and Antioxidant, Antiproliferative, and Anti-Inflammatory Activity of Annona cacans Warm.

Abstract：

:This study evaluated to determine the phenolic and flavonoids contents, and antioxidant, anti-inflammatory, and antiproliferative activity of the hydromethanolic extracts of the leaves, pulp, and seeds of Annona cacans. The isolation and structural identification of the constituent acetogenin, phenolic acid, and flavonoids were also reported. Antioxidant capacity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ethylbenzothiazoline-6-sulfonic acid (ABTS), and β-carotene/linoleic acid methods. Cell proliferation was determined by spectrophotometric quantification of the cellular protein content using a sulforhodamine B assay. Anti-inflammatory activity was evaluated in paw edema model, to myeloperoxidase (MPO) activity induced by carrageenan in mice. Fractionation resulted in the isolation of one acetogenin (annoreticuin-9-one), two flavonoids (quercetin-3-O-β-glucoside-6-O-α-rhamnoside and kaempferol-3-O-β-glucoside), and one phenolic acid (p-coumaric acid). The pulp extract presented potent antioxidant activities by the DPPH (IC50 = 44.08 μg/mL) and ABTS (IC50 = 39.32 μg/mL) methods, as well as high contents of phenols (618.95 mg GA/g) and flavonoids (477.35 mg QE/g). The bioguided fractionation demonstrated that the ethyl acetate fraction of the pulp extract and annoreticuin-9-one showed potent antiproliferative activity against ovarian cancer (GI50 = 6.4 μg/mL). The anti-inflammatory activity demonstrated significant inhibition of edema compared to the control group in 2 and 4 h; in addition, the extracts inhibited the increase in MPO activity after 6 h, when compared to the DEX and control groups. For the first time, this study demonstrated antioxidant, anti-inflammatory, and antiproliferative activity, as well as compounds isolated, suggesting that A. cacans could also be potential sources for prevention of cancer and other diseases associated with oxidative stress.

journal_name

J Med Food

journal_title

Journal of medicinal food

authors

Volobuff CRF,Pederiva MMC,Benites RSR,Lima CJ,Argandoña EJS,Cardoso CAL,Pereira ZV,Ruiz ALTG,Foglio MA,de Carvalho JE,Formagio ASN

doi

10.1089/jmf.2018.0198

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

1078-1086

issue

eissn

1096-620X

issn

1557-7600

journal_volume

pub_type

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Bioguided Fractionation, and Antioxidant, Antiproliferative, and Anti-Inflammatory Activity of Annona cacans Warm.