Abstract:
OBJECTIVE:This study aims to investigate whether HOX transcript antisense RNA (HOTAIR) can participate in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by regulating the Wnt/β-catenin pathway, thereby participating in the pathogenesis of osteoporosis. PATIENTS AND METHODS:We detected the expression level of HOTAIR in 60 osteoporosis patients and 60 normal controls by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Meanwhile, BMSCs derived from human or rats were subjected to determination of HOTAIR level. Subsequently, the effects of HOTAIR on osteogenic differentiation were evaluated by the activity of Alkaline Phosphatase (ALP), Alizarin Red S (ARS) staining, ALP staining and osteogenic-specific gene expression. The expression level of proteins related to the Wnt/β-catenin was determined by Western blot, and ALP activity was detected by ALP activity determination kit and alizarin red staining after knockdown or overexpression of HOTAIR, as well as the treatment of DKK1 or the Wnt pathway antagonist. Finally, osteoporosis model in rats was established by ovariectomy (OVX). We examined protein levels of HOTAIR, β-catenin, CyclinD, C-myc, and Runx2 in rat bone tissues. Bone morphology was observed in each group as well. RESULTS:The serum and BMSCs levels of HOTAIR in patients with osteoporosis were remarkably higher than that in normal people. Inhibition of HOTAIR induced increased ALP activity increased osteogenic marker genes and enhanced number of calcified nodules in BMSCs. However, the overexpression of HOTAIR exhibited the opposite effects. HOTAIR inhibited the expression level of Wnt/β-catenin pathway-related protein. Also, Wnt pathway antagonist DKK1 partially reversed the regulatory effects of HOTAIR on Wnt/β-catenin. DKK1 treatment markedly reduced the promotive effect of HOTAIR knockdown on ALP activity, ALP content and calcification ability of BMSCs. DKK1 administration in rats undergoing OVX showed worse bone morphology relative to controls. Protein levels of HOTAIR, β-catenin, CyclinD, C-myc and Runx2 remarkably downregulated in OVX rats administrated with DKK1. CONCLUSIONS:HOTAIR inhibits osteoblast differentiation of rat BMSCs. The underlying mechanism of which may be related to the mediation of Wnt/β-catenin pathway.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Shen JJ,Zhang CH,Chen ZW,Wang ZX,Yang DC,Zhang FL,Feng KHdoi
10.26355/eurrev_201909_18826subject
Has Abstractpub_date
2019-09-01 00:00:00pages
7232-7246issue
17eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:To explore the expression and function of insulin-like growth factor II (IGFII) mRNA binding protein (IMP3) in the Triple Negative Breast Cancer (TNBC). MATERIALS AND METHODS:According to previously reported gene expression array, we found that IMP3 had significantly higher expression in the CD44+CD24-ESA+ c...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201805_14965
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:Bone loss is the main reason for postmenopausal osteoporosis, caused by estrogen deficiency. ERT (estrogen replacement therapy) has been demonstrated to protect bone loss efficiently. LncRNA (long non-coding RNA) has been proved to be important in different disease progression. We aimed at analyzing whether t...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201809_15907
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abstract:OBJECTIVE:Prostate cancer is a kind of malignancy with high occurrence in the male urogenital system. However, the mechanism of the occurrence, the progression, and the metastasis of prostate cancer are still unclear. Searching for the effective molecule target is of great significance to improve the curative effect on...
journal_title:European review for medical and pharmacological sciences
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abstract::We have studied the temporal bones of 4 deceased donors, individuals one with cochlear saccular degeneration, another with Mondini dysplasia, another with an ossification of the basal turn of the cochlea and the round window, post meningitis and the fourth who was implanted 10 years before. The indications and contrai...
journal_title:European review for medical and pharmacological sciences
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abstract:OBJECTIVE:Familial atrial fibrillation (FAF), a not uncommon arrhythmia of the atrium, is characterized by heritability, early onset and absence of other heart defects. The molecular and genetic basis is still not completely clear and genetic diagnosis cannot be achieved in about 90% of patients. In this study, we pres...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201909_18880
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abstract:OBJECTIVES:Aggressive cell growth and chemoresistance are notorious obstacles in neuroblastoma therapy. Accumulating evidence suggests that survivin is preferentially expressed in cancer cells and plays a crucial role in cell division and apoptosis dysfunction. Thus, in the present study, we investigated whether silenc...
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abstract::This article describes the management of acute asthma exacerbation in the Emergency Department (ED). An asthma exacerbation can be defined as clinical worsening of disease or an asymptomatic decrease in peak flows. Acute exacerbations of asthma may represent reactions to airway irritants or failures of chronic treatme...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
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doi:10.26355/eurrev_202012_24193
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doi:10.26355/eurrev_201904_17567
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journal_title:European review for medical and pharmacological sciences
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更新日期:2014-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2014-01-01 00:00:00
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doi:10.26355/eurrev_202001_20035
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doi:10.26355/eurrev_202101_24397
更新日期:2021-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19435
更新日期:2019-11-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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abstract::No Abstract Available. ...
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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更新日期:2016-07-01 00:00:00
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doi:10.26355/eurrev_202010_23440
更新日期:2020-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2016-01-01 00:00:00
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更新日期:2020-11-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
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