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Difference in the cell proliferation and colony-forming ability of normal human T lymphocytes.

Abstract：

:Cell transfer experiments were carried out with phytohaemagglutinin-induced normal human T lymphocyte colonies after 2--10 days of primary colony growth. The cells gave a cloning efficiency of 15% after 2 days of incubation and this decreased to 0.2% with the progressive growth of the colonies. The primary colonies had a proliferative capacity to give about 240 cells after 10 days of incubation, but only contained 0.5 to one cell per colony that could form a new colony. This number of colony-forming cells per colony did not change with an increase in colony size. These results indicate that colonies maintained the same number of colony-forming cells that they started with and that the other cells could proliferate but not form colonies. It is suggested that this ability to distinguish between colony-forming and proliferative T cells may be useful for determining specific deficiencie in either cell type in various diseases.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Smith SD,Sachs L

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

348-51

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

37

pub_type

杂志文章

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