Abstract：
:One of the main concerns about genetically modified foods and their potential impacts on human health is that the introduction of a new/ altered gene may putatively alter the expression of others, namely endogenous allergens. We intended to evaluate, and to compare, using quantitative real time RT-PCR technique, the expression of 5 already known maize allergens (Zea m14, Zea m25, Zea m27kD, 50kD Zein and trypsin inhibitor) in MON 810 vs. its non-transgenic counterpart, throughout seed development (10, 16 and 23days after pollination). We have shown that none of the tested allergen genes presented differential expression, with statistic significance, along all tested seed development stages, in MON810 vs. its conventional counterpart. We have also used bidimensional gel electrophoresis followed by Western blotting with plasma from two maize allergic subjects to characterize their immunologic responses against MON 810 vs. its non-transgenic control. Immunoreactive spots were characterized by MS. We have identified fourteen new IgE-binding proteins present in both transgenic and non-transgenic maize.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Fonseca C,Planchon S,Renaut J,Oliveira MM,Batista Rdoi
10.1016/j.jprot.2012.01.005subject
Has Abstractpub_date
2012-04-03 00:00:00pages
2027-37issue
7eissn
1874-3919issn
1876-7737pii
S1874-3919(12)00022-Xjournal_volume
75pub_type
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journal_title：Journal of proteomics
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journal_title：Journal of proteomics
pub_type： 杂志文章,评审
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journal_title：Journal of proteomics
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2016.05.004
更新日期：2016-09-16 00:00:00
abstract：UNLABELLED:The pathogenicity of Yersinia enterocolitica biovar 1A strains is controversial as these lack most of the known virulence factors. Acquisition of iron and presence of well-regulated iron homeostasis in bacteria represents an important virulence trait. Differential abundance of proteins was examined under iro...
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Journal of proteomics
pub_type： 杂志文章
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更新日期：2014-08-28 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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更新日期：2012-09-18 00:00:00
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